High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles.
Improved detection of anti-carbohydrate antibodies is a need in clinical identification of biomarkers for cancer cells or pathogens. Here, we report a new ELISA approach for the detection of specific immunoglobulins (IgGs) against carbohydrates. Two nanometer gold glyconanoparticles bearing oligosac...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24014084/pdf/?tool=EBI |
| _version_ | 1818701929107161088 |
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| author | Fabrizio Chiodo Marco Marradi Boris Tefsen Harm Snippe Irma van Die Soledad Penadés |
| author_facet | Fabrizio Chiodo Marco Marradi Boris Tefsen Harm Snippe Irma van Die Soledad Penadés |
| author_sort | Fabrizio Chiodo |
| collection | DOAJ |
| description | Improved detection of anti-carbohydrate antibodies is a need in clinical identification of biomarkers for cancer cells or pathogens. Here, we report a new ELISA approach for the detection of specific immunoglobulins (IgGs) against carbohydrates. Two nanometer gold glyconanoparticles bearing oligosaccharide epitopes of HIV or Streptococcus pneumoniae were used as antigens to coat ELISA-plates. A ~3,000-fold improved detection of specific IgGs in mice immunized against S. pneumoniae respect to the well known BSA-glycoconjugate ELISA was achieved. Moreover, these multivalent glyconanoparticles have been employed in solid phase assays to detect the carbohydrate-dependent binding of human dendritic cells and the lectin DC-SIGN. Multivalent glyconanoparticles in ELISA provide a versatile, easy and highly sensitive method to detect and quantify the binding of glycan to proteins and to facilitate the identification of biomarkers. |
| first_indexed | 2024-12-17T15:28:38Z |
| format | Article |
| id | doaj.art-8b2430224ce84b2abaf8bffba18c9b0d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T15:28:38Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8b2430224ce84b2abaf8bffba18c9b0d2022-12-21T21:43:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7302710.1371/journal.pone.0073027High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles.Fabrizio ChiodoMarco MarradiBoris TefsenHarm SnippeIrma van DieSoledad PenadésImproved detection of anti-carbohydrate antibodies is a need in clinical identification of biomarkers for cancer cells or pathogens. Here, we report a new ELISA approach for the detection of specific immunoglobulins (IgGs) against carbohydrates. Two nanometer gold glyconanoparticles bearing oligosaccharide epitopes of HIV or Streptococcus pneumoniae were used as antigens to coat ELISA-plates. A ~3,000-fold improved detection of specific IgGs in mice immunized against S. pneumoniae respect to the well known BSA-glycoconjugate ELISA was achieved. Moreover, these multivalent glyconanoparticles have been employed in solid phase assays to detect the carbohydrate-dependent binding of human dendritic cells and the lectin DC-SIGN. Multivalent glyconanoparticles in ELISA provide a versatile, easy and highly sensitive method to detect and quantify the binding of glycan to proteins and to facilitate the identification of biomarkers.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24014084/pdf/?tool=EBI |
| spellingShingle | Fabrizio Chiodo Marco Marradi Boris Tefsen Harm Snippe Irma van Die Soledad Penadés High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles. PLoS ONE |
| title | High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles. |
| title_full | High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles. |
| title_fullStr | High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles. |
| title_full_unstemmed | High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles. |
| title_short | High sensitive detection of carbohydrate binding proteins in an ELISA-solid phase assay based on multivalent glyconanoparticles. |
| title_sort | high sensitive detection of carbohydrate binding proteins in an elisa solid phase assay based on multivalent glyconanoparticles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24014084/pdf/?tool=EBI |
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