Engineered 3D liver-tissue model with minispheroids formed by a bioprinting process supported with in situ electrical stimulation
Three-dimensional (3D) bioprinting, an effective technique for building cell-laden structures providing native extracellular matrix environments, presents challenges, including inadequate cellular interactions. To address these issues, cell spheroids offer a promising solution for improving their bi...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-05-01
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| Series: | Bioactive Materials |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X24000458 |
| _version_ | 1797235545090293760 |
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| author | WonJin Kim GeunHyung Kim |
| author_facet | WonJin Kim GeunHyung Kim |
| author_sort | WonJin Kim |
| collection | DOAJ |
| description | Three-dimensional (3D) bioprinting, an effective technique for building cell-laden structures providing native extracellular matrix environments, presents challenges, including inadequate cellular interactions. To address these issues, cell spheroids offer a promising solution for improving their biological functions. Particularly, minispheroids with 50–100 μm diameters exhibit enhanced cellular maturation. We propose a one-step minispheroid-forming bioprinting process incorporating electrical stimulation (E-MS-printing). By stimulating the cells, minispheroids with controlled diameters were generated by manipulating the bioink viscosity and stimulation intensity. To validate its feasibility, E-MS-printing process was applied to fabricate an engineered liver model designed to mimic the hepatic lobule unit. E-MS-printing was employed to print the hepatocyte region, followed by bioprinting the central vein using a core-shell nozzle. The resulting constructs displayed native liver-mimetic structures containing minispheroids, which facilitated improved hepatic cell maturation, functional attributes, and vessel formation. Our results demonstrate a new potential 3D liver model that can replicate native liver tissues. |
| first_indexed | 2024-03-08T00:49:11Z |
| format | Article |
| id | doaj.art-8b25651ce6e9492d96667a8a9f253ccc |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T16:49:39Z |
| publishDate | 2024-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-8b25651ce6e9492d96667a8a9f253ccc2024-03-29T05:50:54ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2024-05-0135382400Engineered 3D liver-tissue model with minispheroids formed by a bioprinting process supported with in situ electrical stimulationWonJin Kim0GeunHyung Kim1Department of Precision Medicine, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of KoreaDepartment of Precision Medicine, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of Korea; Institute of Quantum Biophysics, Department of Biophysics, Sungkyunkwan University, Suwon, 16419, Republic of Korea; Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon, 16419, Republic of Korea; Corresponding author. Department of Precision Medicine, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of Korea.Three-dimensional (3D) bioprinting, an effective technique for building cell-laden structures providing native extracellular matrix environments, presents challenges, including inadequate cellular interactions. To address these issues, cell spheroids offer a promising solution for improving their biological functions. Particularly, minispheroids with 50–100 μm diameters exhibit enhanced cellular maturation. We propose a one-step minispheroid-forming bioprinting process incorporating electrical stimulation (E-MS-printing). By stimulating the cells, minispheroids with controlled diameters were generated by manipulating the bioink viscosity and stimulation intensity. To validate its feasibility, E-MS-printing process was applied to fabricate an engineered liver model designed to mimic the hepatic lobule unit. E-MS-printing was employed to print the hepatocyte region, followed by bioprinting the central vein using a core-shell nozzle. The resulting constructs displayed native liver-mimetic structures containing minispheroids, which facilitated improved hepatic cell maturation, functional attributes, and vessel formation. Our results demonstrate a new potential 3D liver model that can replicate native liver tissues.http://www.sciencedirect.com/science/article/pii/S2452199X24000458BioprintingIn situ electric fieldMinispheroidsIn vitro liver-tissue modelHepatic lobule |
| spellingShingle | WonJin Kim GeunHyung Kim Engineered 3D liver-tissue model with minispheroids formed by a bioprinting process supported with in situ electrical stimulation Bioactive Materials Bioprinting In situ electric field Minispheroids In vitro liver-tissue model Hepatic lobule |
| title | Engineered 3D liver-tissue model with minispheroids formed by a bioprinting process supported with in situ electrical stimulation |
| title_full | Engineered 3D liver-tissue model with minispheroids formed by a bioprinting process supported with in situ electrical stimulation |
| title_fullStr | Engineered 3D liver-tissue model with minispheroids formed by a bioprinting process supported with in situ electrical stimulation |
| title_full_unstemmed | Engineered 3D liver-tissue model with minispheroids formed by a bioprinting process supported with in situ electrical stimulation |
| title_short | Engineered 3D liver-tissue model with minispheroids formed by a bioprinting process supported with in situ electrical stimulation |
| title_sort | engineered 3d liver tissue model with minispheroids formed by a bioprinting process supported with in situ electrical stimulation |
| topic | Bioprinting In situ electric field Minispheroids In vitro liver-tissue model Hepatic lobule |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X24000458 |
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