Effect of Dinotefuran, Permethrin, and Pyriproxyfen (Vectra<sup>®</sup> 3D) on the Foraging and Blood-Feeding Behaviors of <i>Aedes albopictus</i> Using Laboratory Rodent Model
Dinotefuran-Permethrin-Pyriproxyfen (DPP) is used to kill and repel mosquitoes from dogs. However, the influence of the product on the host-seeking behavior of mosquitoes remains unknown. The interference of DPP with the host selection of unfed female <i>Aedes albopictus</i> was investig...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/8/507 |
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| author | Younes Laidoudi Djamel Tahir Hacène Medkour Marie Varloud Oleg Mediannikov Bernard Davoust |
| author_facet | Younes Laidoudi Djamel Tahir Hacène Medkour Marie Varloud Oleg Mediannikov Bernard Davoust |
| author_sort | Younes Laidoudi |
| collection | DOAJ |
| description | Dinotefuran-Permethrin-Pyriproxyfen (DPP) is used to kill and repel mosquitoes from dogs. However, the influence of the product on the host-seeking behavior of mosquitoes remains unknown. The interference of DPP with the host selection of unfed female <i>Aedes albopictus</i> was investigated. A total of 18 animals (9 mice and 9 rats) were divided into three groups of six animals each. DU: DPP treated rats (<i>n</i> = 3) with untreated mice (<i>n</i> = 3), UD: DPP treated mice (<i>n</i> = 3) with untreated rats (<i>n</i> = 3) and control UU: untreated mice (<i>n</i> = 3) and untreated rats (<i>n</i> = 3). In each group, the rats and mice were placed 30 cm apart. After sedation, the animals in each group were exposed twice (Day 1 and Day 7 post-treatment) for one hour to 71 ± 3 female mosquitoes. Mosquitoes were categorized after the 2-h post-exposure period as dead or alive. Blood-meal origin was determined from mosquitoes using a newly customized duplex qPCR. The highest values of forage ratio (1.36 ≥ <i>wi</i> ≤ 1.88) and selection index (0.63 ≥ <i>Bi</i> ≤ 0.94) for rat hosts indicates a preference of mosquitoes for this species as compared to mice when co-housed during the exposure. The mosquitoes only seldom fed on mice, even in the untreated group. The anti-feeding effect of DPP was therefore only assessed on rat’s hosts. The results showed that DPP, when directly applied on rats, provided a direct protection of 82% and 61% on Day 1 and Day 7, respectively, while when applied on mice hosts (UD), the DPP provided an indirect protection of 21% and 10% on Day 1 and Day 7, respectively. The results showed also that DPP, when applied on rats, provided a direct protection against <i>Ae. albopictus</i> bites. This effect did not result in increased exposure of the untreated host placed in the same cage at a distance of 30 cm. |
| first_indexed | 2024-03-10T17:56:06Z |
| format | Article |
| id | doaj.art-8b25ffc4363b4496b34a7e7445e207d3 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T17:56:06Z |
| publishDate | 2020-08-01 |
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| record_format | Article |
| series | Insects |
| spelling | doaj.art-8b25ffc4363b4496b34a7e7445e207d32023-11-20T09:10:55ZengMDPI AGInsects2075-44502020-08-0111850710.3390/insects11080507Effect of Dinotefuran, Permethrin, and Pyriproxyfen (Vectra<sup>®</sup> 3D) on the Foraging and Blood-Feeding Behaviors of <i>Aedes albopictus</i> Using Laboratory Rodent ModelYounes Laidoudi0Djamel Tahir1Hacène Medkour2Marie Varloud3Oleg Mediannikov4Bernard Davoust5AP-HM, Microbes, Evolution, Phylogeny and Infection (MEPHI), IHU Méditerranée Infection, IRD, Aix Marseille Univ, 19-21, Bd Jean Moulin, 13005 Marseille, FranceAP-HM, Microbes, Evolution, Phylogeny and Infection (MEPHI), IHU Méditerranée Infection, IRD, Aix Marseille Univ, 19-21, Bd Jean Moulin, 13005 Marseille, FranceAP-HM, Microbes, Evolution, Phylogeny and Infection (MEPHI), IHU Méditerranée Infection, IRD, Aix Marseille Univ, 19-21, Bd Jean Moulin, 13005 Marseille, FranceCeva Santé Animale, 10, Av de la Ballastière, 33500 Libourne, FranceAP-HM, Microbes, Evolution, Phylogeny and Infection (MEPHI), IHU Méditerranée Infection, IRD, Aix Marseille Univ, 19-21, Bd Jean Moulin, 13005 Marseille, FranceAP-HM, Microbes, Evolution, Phylogeny and Infection (MEPHI), IHU Méditerranée Infection, IRD, Aix Marseille Univ, 19-21, Bd Jean Moulin, 13005 Marseille, FranceDinotefuran-Permethrin-Pyriproxyfen (DPP) is used to kill and repel mosquitoes from dogs. However, the influence of the product on the host-seeking behavior of mosquitoes remains unknown. The interference of DPP with the host selection of unfed female <i>Aedes albopictus</i> was investigated. A total of 18 animals (9 mice and 9 rats) were divided into three groups of six animals each. DU: DPP treated rats (<i>n</i> = 3) with untreated mice (<i>n</i> = 3), UD: DPP treated mice (<i>n</i> = 3) with untreated rats (<i>n</i> = 3) and control UU: untreated mice (<i>n</i> = 3) and untreated rats (<i>n</i> = 3). In each group, the rats and mice were placed 30 cm apart. After sedation, the animals in each group were exposed twice (Day 1 and Day 7 post-treatment) for one hour to 71 ± 3 female mosquitoes. Mosquitoes were categorized after the 2-h post-exposure period as dead or alive. Blood-meal origin was determined from mosquitoes using a newly customized duplex qPCR. The highest values of forage ratio (1.36 ≥ <i>wi</i> ≤ 1.88) and selection index (0.63 ≥ <i>Bi</i> ≤ 0.94) for rat hosts indicates a preference of mosquitoes for this species as compared to mice when co-housed during the exposure. The mosquitoes only seldom fed on mice, even in the untreated group. The anti-feeding effect of DPP was therefore only assessed on rat’s hosts. The results showed that DPP, when directly applied on rats, provided a direct protection of 82% and 61% on Day 1 and Day 7, respectively, while when applied on mice hosts (UD), the DPP provided an indirect protection of 21% and 10% on Day 1 and Day 7, respectively. The results showed also that DPP, when applied on rats, provided a direct protection against <i>Ae. albopictus</i> bites. This effect did not result in increased exposure of the untreated host placed in the same cage at a distance of 30 cm.https://www.mdpi.com/2075-4450/11/8/507<i>Aedes albopictus</i>animal modelhost selectionblood-meal identificationrodent-qPCRVectra<sup>®</sup> 3D |
| spellingShingle | Younes Laidoudi Djamel Tahir Hacène Medkour Marie Varloud Oleg Mediannikov Bernard Davoust Effect of Dinotefuran, Permethrin, and Pyriproxyfen (Vectra<sup>®</sup> 3D) on the Foraging and Blood-Feeding Behaviors of <i>Aedes albopictus</i> Using Laboratory Rodent Model Insects <i>Aedes albopictus</i> animal model host selection blood-meal identification rodent-qPCR Vectra<sup>®</sup> 3D |
| title | Effect of Dinotefuran, Permethrin, and Pyriproxyfen (Vectra<sup>®</sup> 3D) on the Foraging and Blood-Feeding Behaviors of <i>Aedes albopictus</i> Using Laboratory Rodent Model |
| title_full | Effect of Dinotefuran, Permethrin, and Pyriproxyfen (Vectra<sup>®</sup> 3D) on the Foraging and Blood-Feeding Behaviors of <i>Aedes albopictus</i> Using Laboratory Rodent Model |
| title_fullStr | Effect of Dinotefuran, Permethrin, and Pyriproxyfen (Vectra<sup>®</sup> 3D) on the Foraging and Blood-Feeding Behaviors of <i>Aedes albopictus</i> Using Laboratory Rodent Model |
| title_full_unstemmed | Effect of Dinotefuran, Permethrin, and Pyriproxyfen (Vectra<sup>®</sup> 3D) on the Foraging and Blood-Feeding Behaviors of <i>Aedes albopictus</i> Using Laboratory Rodent Model |
| title_short | Effect of Dinotefuran, Permethrin, and Pyriproxyfen (Vectra<sup>®</sup> 3D) on the Foraging and Blood-Feeding Behaviors of <i>Aedes albopictus</i> Using Laboratory Rodent Model |
| title_sort | effect of dinotefuran permethrin and pyriproxyfen vectra sup r sup 3d on the foraging and blood feeding behaviors of i aedes albopictus i using laboratory rodent model |
| topic | <i>Aedes albopictus</i> animal model host selection blood-meal identification rodent-qPCR Vectra<sup>®</sup> 3D |
| url | https://www.mdpi.com/2075-4450/11/8/507 |
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