ANTIOXIDATIVE EFFECT OF EPIGALLOCATECHIN GALLATE AGAINST D-GALACTOSAMINE-INDUCED INJURY IN PRIMARY CULTURE OF RAT HEPATOCYTES
Literature data support that green tea and its major component epigallocatechin gallate (EGCG) have powerful antioxidant effects. Contrary, hepatotoxicity can be induced by high-dose EGCG. The timing of exposure to green tea in relation to administration of hepatotoxic agent plays an import role too...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Karolinum Press
2014-01-01
|
| Series: | Acta Medica |
| Subjects: | |
| Online Access: | https://actamedica.lfhk.cuni.cz/57/1/0003/ |
| _version_ | 1818886679587454976 |
|---|---|
| author | Alena Moravcová Zuzana Červinková Otto Kučera Vojtěch Mezera Halka Lotková |
| author_facet | Alena Moravcová Zuzana Červinková Otto Kučera Vojtěch Mezera Halka Lotková |
| author_sort | Alena Moravcová |
| collection | DOAJ |
| description | Literature data support that green tea and its major component epigallocatechin gallate (EGCG) have powerful antioxidant effects. Contrary, hepatotoxicity can be induced by high-dose EGCG. The timing of exposure to green tea in relation to administration of hepatotoxic agent plays an import role too. The aim of our work was a verification of antioxidative effect of EGCG on D-galactosamine-induced injury in primary culture of rat hepatocytes. Hepatocytes were incubated with EGCG at concentrations of 1.25–10 μM and toxic D-galactosamine (GalN) for 24 hrs. Alternatively, hepatocytes were pretreated with EGCG for 24 hrs, and then incubated with EGCG and GalN for further 24 hrs. Cytotoxicity was analysed by lactate dehydrogenase activity, functional capacity by albumin production. Oxidative stress was evaluated from a production of malondialdehyde and glutathione content in the cells. EGCG protected hepatocytes against GalN-induced cytotoxicity but preventive treatment of intact hepatocytes with EGCG was required to diminish the development of hepatocyte injury. Oxidative stress induced in our study seems to overcome the ability of hepatocytes to improve GSH depletion and albumin production. Prolongation of the pretreatment with EGCG could be a promising strategy leading to amelioration of its hepatoprotective effect. |
| first_indexed | 2024-12-19T16:25:10Z |
| format | Article |
| id | doaj.art-8b2968bd68894ee386c3e4df61f5418f |
| institution | Directory Open Access Journal |
| issn | 1211-4286 1805-9694 |
| language | English |
| last_indexed | 2024-12-19T16:25:10Z |
| publishDate | 2014-01-01 |
| publisher | Karolinum Press |
| record_format | Article |
| series | Acta Medica |
| spelling | doaj.art-8b2968bd68894ee386c3e4df61f5418f2022-12-21T20:14:23ZengKarolinum PressActa Medica1211-42861805-96942014-01-015713810.14712/18059694.2014.1ANTIOXIDATIVE EFFECT OF EPIGALLOCATECHIN GALLATE AGAINST D-GALACTOSAMINE-INDUCED INJURY IN PRIMARY CULTURE OF RAT HEPATOCYTESAlena Moravcová0Zuzana Červinková1Otto Kučera2Vojtěch Mezera3Halka Lotková4Charles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic: Department of PhysiologyCharles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic: Department of PhysiologyCharles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic: Department of PhysiologyCharles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic: Department of PhysiologyCharles University in Prague, Faculty of Medicine in Hradec Králové, Czech Republic: Department of PhysiologyLiterature data support that green tea and its major component epigallocatechin gallate (EGCG) have powerful antioxidant effects. Contrary, hepatotoxicity can be induced by high-dose EGCG. The timing of exposure to green tea in relation to administration of hepatotoxic agent plays an import role too. The aim of our work was a verification of antioxidative effect of EGCG on D-galactosamine-induced injury in primary culture of rat hepatocytes. Hepatocytes were incubated with EGCG at concentrations of 1.25–10 μM and toxic D-galactosamine (GalN) for 24 hrs. Alternatively, hepatocytes were pretreated with EGCG for 24 hrs, and then incubated with EGCG and GalN for further 24 hrs. Cytotoxicity was analysed by lactate dehydrogenase activity, functional capacity by albumin production. Oxidative stress was evaluated from a production of malondialdehyde and glutathione content in the cells. EGCG protected hepatocytes against GalN-induced cytotoxicity but preventive treatment of intact hepatocytes with EGCG was required to diminish the development of hepatocyte injury. Oxidative stress induced in our study seems to overcome the ability of hepatocytes to improve GSH depletion and albumin production. Prolongation of the pretreatment with EGCG could be a promising strategy leading to amelioration of its hepatoprotective effect.https://actamedica.lfhk.cuni.cz/57/1/0003/Epigallocatechin gallateHepatocytesD-galactosamineOxidative stress |
| spellingShingle | Alena Moravcová Zuzana Červinková Otto Kučera Vojtěch Mezera Halka Lotková ANTIOXIDATIVE EFFECT OF EPIGALLOCATECHIN GALLATE AGAINST D-GALACTOSAMINE-INDUCED INJURY IN PRIMARY CULTURE OF RAT HEPATOCYTES Acta Medica Epigallocatechin gallate Hepatocytes D-galactosamine Oxidative stress |
| title | ANTIOXIDATIVE EFFECT OF EPIGALLOCATECHIN GALLATE AGAINST D-GALACTOSAMINE-INDUCED INJURY IN PRIMARY CULTURE OF RAT HEPATOCYTES |
| title_full | ANTIOXIDATIVE EFFECT OF EPIGALLOCATECHIN GALLATE AGAINST D-GALACTOSAMINE-INDUCED INJURY IN PRIMARY CULTURE OF RAT HEPATOCYTES |
| title_fullStr | ANTIOXIDATIVE EFFECT OF EPIGALLOCATECHIN GALLATE AGAINST D-GALACTOSAMINE-INDUCED INJURY IN PRIMARY CULTURE OF RAT HEPATOCYTES |
| title_full_unstemmed | ANTIOXIDATIVE EFFECT OF EPIGALLOCATECHIN GALLATE AGAINST D-GALACTOSAMINE-INDUCED INJURY IN PRIMARY CULTURE OF RAT HEPATOCYTES |
| title_short | ANTIOXIDATIVE EFFECT OF EPIGALLOCATECHIN GALLATE AGAINST D-GALACTOSAMINE-INDUCED INJURY IN PRIMARY CULTURE OF RAT HEPATOCYTES |
| title_sort | antioxidative effect of epigallocatechin gallate against d galactosamine induced injury in primary culture of rat hepatocytes |
| topic | Epigallocatechin gallate Hepatocytes D-galactosamine Oxidative stress |
| url | https://actamedica.lfhk.cuni.cz/57/1/0003/ |
| work_keys_str_mv | AT alenamoravcova antioxidativeeffectofepigallocatechingallateagainstdgalactosamineinducedinjuryinprimarycultureofrathepatocytes AT zuzanacervinkova antioxidativeeffectofepigallocatechingallateagainstdgalactosamineinducedinjuryinprimarycultureofrathepatocytes AT ottokucera antioxidativeeffectofepigallocatechingallateagainstdgalactosamineinducedinjuryinprimarycultureofrathepatocytes AT vojtechmezera antioxidativeeffectofepigallocatechingallateagainstdgalactosamineinducedinjuryinprimarycultureofrathepatocytes AT halkalotkova antioxidativeeffectofepigallocatechingallateagainstdgalactosamineinducedinjuryinprimarycultureofrathepatocytes |