“Two Birds with One Stone” Ruthenium(II) Complex Probe for Biothiols Discrimination and Detection In Vitro and In Vivo
Abstract In this work, a “two birds with one stone” ruthenium(II) complex probe, Ru‐NBD, is proposed as an effective tool for biothiols detection and discrimination in vitro and in vivo. Ru‐NBD is nonluminescent due to the quenching of Ru(II) complex emission by photoinduced electron transfer (PET)...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-07-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202000458 |
| _version_ | 1819228921166561280 |
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| author | Chaolong Liu Jianping Liu Wenzhu Zhang Yong‐Lei Wang Qi Liu Bo Song Jingli Yuan Run Zhang |
| author_facet | Chaolong Liu Jianping Liu Wenzhu Zhang Yong‐Lei Wang Qi Liu Bo Song Jingli Yuan Run Zhang |
| author_sort | Chaolong Liu |
| collection | DOAJ |
| description | Abstract In this work, a “two birds with one stone” ruthenium(II) complex probe, Ru‐NBD, is proposed as an effective tool for biothiols detection and discrimination in vitro and in vivo. Ru‐NBD is nonluminescent due to the quenching of Ru(II) complex emission by photoinduced electron transfer (PET) from Ru(II) center to NBD and the quenching of NBD emission through 4‐substitution with “O” ether bond. Ru‐NBD is capable of reacting with Cys/Hcy to form long‐lived red‐emitting Ru‐OH and short‐lived green‐emitting NBD‐NR, while reacting with GSH to produce Ru‐OH and nonemissive NBD‐SR. The long lifetime emission of Ru(II) complex allows elimination of short lifetime background and NBD‐NR fluorescence for total biothiols detection (“bird” one) by time‐gated luminescence (TGL) analysis, and the remarkable difference in luminescence color response allows discrimination GSH and Cys/Hcy (“bird” two) through steady‐state luminescence analysis. Ru‐NBD features high sensitivity and selectivity, rapid luminescence response, and low cytotoxicity, which enables it to be used as the probe for luminescence and background‐free TGL detection and visualization of biothiols in live cells, zebrafish, and mice. The successful development of this probe is anticipated to contribute to the future biological studies of biothiols roles in various diseases. |
| first_indexed | 2024-12-23T11:04:57Z |
| format | Article |
| id | doaj.art-8b2de563bcaf41c98016c740aa66b460 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-12-23T11:04:57Z |
| publishDate | 2020-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-8b2de563bcaf41c98016c740aa66b4602022-12-21T17:49:30ZengWileyAdvanced Science2198-38442020-07-01714n/an/a10.1002/advs.202000458“Two Birds with One Stone” Ruthenium(II) Complex Probe for Biothiols Discrimination and Detection In Vitro and In VivoChaolong Liu0Jianping Liu1Wenzhu Zhang2Yong‐Lei Wang3Qi Liu4Bo Song5Jingli Yuan6Run Zhang7State Key Laboratory of Fine Chemicals School of Chemical Engineering Dalian University of Technology Dalian 116024 ChinaAustralian Institute for Bioengineering and Nanotechnology The University of Queensland, St. Lucia Brisbane QLD 4072 AustraliaState Key Laboratory of Fine Chemicals School of Chemical Engineering Dalian University of Technology Dalian 116024 ChinaDepartment of Materials and Environmental Chemistry Stockholm University Stockholm SE‐10691 SwedenState Key Laboratory of Fine Chemicals School of Chemical Engineering Dalian University of Technology Dalian 116024 ChinaState Key Laboratory of Fine Chemicals School of Chemical Engineering Dalian University of Technology Dalian 116024 ChinaState Key Laboratory of Fine Chemicals School of Chemical Engineering Dalian University of Technology Dalian 116024 ChinaAustralian Institute for Bioengineering and Nanotechnology The University of Queensland, St. Lucia Brisbane QLD 4072 AustraliaAbstract In this work, a “two birds with one stone” ruthenium(II) complex probe, Ru‐NBD, is proposed as an effective tool for biothiols detection and discrimination in vitro and in vivo. Ru‐NBD is nonluminescent due to the quenching of Ru(II) complex emission by photoinduced electron transfer (PET) from Ru(II) center to NBD and the quenching of NBD emission through 4‐substitution with “O” ether bond. Ru‐NBD is capable of reacting with Cys/Hcy to form long‐lived red‐emitting Ru‐OH and short‐lived green‐emitting NBD‐NR, while reacting with GSH to produce Ru‐OH and nonemissive NBD‐SR. The long lifetime emission of Ru(II) complex allows elimination of short lifetime background and NBD‐NR fluorescence for total biothiols detection (“bird” one) by time‐gated luminescence (TGL) analysis, and the remarkable difference in luminescence color response allows discrimination GSH and Cys/Hcy (“bird” two) through steady‐state luminescence analysis. Ru‐NBD features high sensitivity and selectivity, rapid luminescence response, and low cytotoxicity, which enables it to be used as the probe for luminescence and background‐free TGL detection and visualization of biothiols in live cells, zebrafish, and mice. The successful development of this probe is anticipated to contribute to the future biological studies of biothiols roles in various diseases.https://doi.org/10.1002/advs.202000458biothiolsruthenium complexestime‐gated luminescencetwo birds with one stone probes |
| spellingShingle | Chaolong Liu Jianping Liu Wenzhu Zhang Yong‐Lei Wang Qi Liu Bo Song Jingli Yuan Run Zhang “Two Birds with One Stone” Ruthenium(II) Complex Probe for Biothiols Discrimination and Detection In Vitro and In Vivo Advanced Science biothiols ruthenium complexes time‐gated luminescence two birds with one stone probes |
| title | “Two Birds with One Stone” Ruthenium(II) Complex Probe for Biothiols Discrimination and Detection In Vitro and In Vivo |
| title_full | “Two Birds with One Stone” Ruthenium(II) Complex Probe for Biothiols Discrimination and Detection In Vitro and In Vivo |
| title_fullStr | “Two Birds with One Stone” Ruthenium(II) Complex Probe for Biothiols Discrimination and Detection In Vitro and In Vivo |
| title_full_unstemmed | “Two Birds with One Stone” Ruthenium(II) Complex Probe for Biothiols Discrimination and Detection In Vitro and In Vivo |
| title_short | “Two Birds with One Stone” Ruthenium(II) Complex Probe for Biothiols Discrimination and Detection In Vitro and In Vivo |
| title_sort | two birds with one stone ruthenium ii complex probe for biothiols discrimination and detection in vitro and in vivo |
| topic | biothiols ruthenium complexes time‐gated luminescence two birds with one stone probes |
| url | https://doi.org/10.1002/advs.202000458 |
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