Time-Dependent Internalization of Polymer-Coated Silica Nanoparticles in Brain Endothelial Cells and Morphological and Functional Effects on the Blood-Brain Barrier
Nanoparticle (NP)-assisted procedures including laser tissue soldering (LTS) offer advantages compared to conventional microsuturing, especially in the brain. In this study, effects of polymer-coated silica NPs used in LTS were investigated in human brain endothelial cells (ECs) and blood-brain barr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/4/1657 |
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| author | Aniela Bittner Fabien Gosselet Emmanuel Sevin Lucie Dehouck Angélique D. Ducray Véronique Gaschen Michael H. Stoffel Hansang Cho Meike Mevissen |
| author_facet | Aniela Bittner Fabien Gosselet Emmanuel Sevin Lucie Dehouck Angélique D. Ducray Véronique Gaschen Michael H. Stoffel Hansang Cho Meike Mevissen |
| author_sort | Aniela Bittner |
| collection | DOAJ |
| description | Nanoparticle (NP)-assisted procedures including laser tissue soldering (LTS) offer advantages compared to conventional microsuturing, especially in the brain. In this study, effects of polymer-coated silica NPs used in LTS were investigated in human brain endothelial cells (ECs) and blood-brain barrier models. In the co-culture setting with ECs and pericytes, only the cell type directly exposed to NPs displayed a time-dependent internalization. No transfer of NPs between the two cell types was observed. Cell viability was decreased relatively to NP exposure duration and concentration. Protein expression of the nuclear factor ĸ-light-chain-enhancer of activated B cells and various endothelial adhesion molecules indicated no initiation of inflammation or activation of ECs after NP exposure. Differentiation of CD34+ ECs into brain-like ECs co-cultured with pericytes, blood-brain barrier (BBB) characteristics were obtained. The established endothelial layer reduced the passage of integrity tracer molecules. NP exposure did not result in alterations of junctional proteins, BBB formation or its integrity. In a 3-dimensional setup with an endothelial tube formation and tight junctions, barrier formation was not disrupted by the NPs and NPs do not seem to cross the blood-brain barrier. Our findings suggest that these polymer-coated silica NPs do not damage the BBB. |
| first_indexed | 2024-03-09T05:20:15Z |
| format | Article |
| id | doaj.art-8b2e1413ffe347b1b2dc89281fa3114b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:20:15Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8b2e1413ffe347b1b2dc89281fa3114b2023-12-03T12:41:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01224165710.3390/ijms22041657Time-Dependent Internalization of Polymer-Coated Silica Nanoparticles in Brain Endothelial Cells and Morphological and Functional Effects on the Blood-Brain BarrierAniela Bittner0Fabien Gosselet1Emmanuel Sevin2Lucie Dehouck3Angélique D. Ducray4Véronique Gaschen5Michael H. Stoffel6Hansang Cho7Meike Mevissen8Division of Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University of Bern, Länggassstrasse 124, 3012 Bern, SwitzerlandBlood-Brain-Barrier Laboratory, University of Artois, UR265, Faculté Jean Perrin, Rue Jean Souvraz–SP 18, 62307 Lens, FranceBlood-Brain-Barrier Laboratory, University of Artois, UR265, Faculté Jean Perrin, Rue Jean Souvraz–SP 18, 62307 Lens, FranceBlood-Brain-Barrier Laboratory, University of Artois, UR265, Faculté Jean Perrin, Rue Jean Souvraz–SP 18, 62307 Lens, FranceDivision of Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University of Bern, Länggassstrasse 124, 3012 Bern, SwitzerlandDivision of Veterinary Anatomy, Vetsuisse Faculty, University of Bern, Länggassstrasse 120, 3012 Bern, SwitzerlandDivision of Veterinary Anatomy, Vetsuisse Faculty, University of Bern, Länggassstrasse 120, 3012 Bern, SwitzerlandInstitute of Quantum Biophysics, Department of Biophysics, Department of Intelligent Precision Healthcare Concergence, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, #868715 N-Center Suwon-si, Gyeonggi-do 16419, KoreaDivision of Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University of Bern, Länggassstrasse 124, 3012 Bern, SwitzerlandNanoparticle (NP)-assisted procedures including laser tissue soldering (LTS) offer advantages compared to conventional microsuturing, especially in the brain. In this study, effects of polymer-coated silica NPs used in LTS were investigated in human brain endothelial cells (ECs) and blood-brain barrier models. In the co-culture setting with ECs and pericytes, only the cell type directly exposed to NPs displayed a time-dependent internalization. No transfer of NPs between the two cell types was observed. Cell viability was decreased relatively to NP exposure duration and concentration. Protein expression of the nuclear factor ĸ-light-chain-enhancer of activated B cells and various endothelial adhesion molecules indicated no initiation of inflammation or activation of ECs after NP exposure. Differentiation of CD34+ ECs into brain-like ECs co-cultured with pericytes, blood-brain barrier (BBB) characteristics were obtained. The established endothelial layer reduced the passage of integrity tracer molecules. NP exposure did not result in alterations of junctional proteins, BBB formation or its integrity. In a 3-dimensional setup with an endothelial tube formation and tight junctions, barrier formation was not disrupted by the NPs and NPs do not seem to cross the blood-brain barrier. Our findings suggest that these polymer-coated silica NPs do not damage the BBB.https://www.mdpi.com/1422-0067/22/4/1657co-culture3D modelpermeabilitytransendothelial electrical resistance |
| spellingShingle | Aniela Bittner Fabien Gosselet Emmanuel Sevin Lucie Dehouck Angélique D. Ducray Véronique Gaschen Michael H. Stoffel Hansang Cho Meike Mevissen Time-Dependent Internalization of Polymer-Coated Silica Nanoparticles in Brain Endothelial Cells and Morphological and Functional Effects on the Blood-Brain Barrier International Journal of Molecular Sciences co-culture 3D model permeability transendothelial electrical resistance |
| title | Time-Dependent Internalization of Polymer-Coated Silica Nanoparticles in Brain Endothelial Cells and Morphological and Functional Effects on the Blood-Brain Barrier |
| title_full | Time-Dependent Internalization of Polymer-Coated Silica Nanoparticles in Brain Endothelial Cells and Morphological and Functional Effects on the Blood-Brain Barrier |
| title_fullStr | Time-Dependent Internalization of Polymer-Coated Silica Nanoparticles in Brain Endothelial Cells and Morphological and Functional Effects on the Blood-Brain Barrier |
| title_full_unstemmed | Time-Dependent Internalization of Polymer-Coated Silica Nanoparticles in Brain Endothelial Cells and Morphological and Functional Effects on the Blood-Brain Barrier |
| title_short | Time-Dependent Internalization of Polymer-Coated Silica Nanoparticles in Brain Endothelial Cells and Morphological and Functional Effects on the Blood-Brain Barrier |
| title_sort | time dependent internalization of polymer coated silica nanoparticles in brain endothelial cells and morphological and functional effects on the blood brain barrier |
| topic | co-culture 3D model permeability transendothelial electrical resistance |
| url | https://www.mdpi.com/1422-0067/22/4/1657 |
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