The Inhibitory Mechanism of Eugenol on <i>Lasiodiplodia theobromae</i> and Its Induced Disease Resistance of Passion Fruit
The inhibitory effects and mechanisms of eugenol were studied in pathogenic fungi that were isolated from passion fruit affected with naturally occurring fruit rot; additionally, the effect of eugenol treatment on fruit rot resistance in passion fruit was investigated. The corresponding results demo...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/13/5/1408 |
| _version_ | 1797601426035179520 |
|---|---|
| author | Yanzheng Sun Liang Shuai Donglan Luo Liangjie Ba |
| author_facet | Yanzheng Sun Liang Shuai Donglan Luo Liangjie Ba |
| author_sort | Yanzheng Sun |
| collection | DOAJ |
| description | The inhibitory effects and mechanisms of eugenol were studied in pathogenic fungi that were isolated from passion fruit affected with naturally occurring fruit rot; additionally, the effect of eugenol treatment on fruit rot resistance in passion fruit was investigated. The corresponding results demonstrated that the pathogenic fungus causing passion fruit rot was <i>Lasiodiplodia theobromae</i> and that the minimal inhibitory concentration of eugenol against <i>L. theobromae</i> was 0.30 mg/mL. Eugenol significantly inhibited mycelial growth and spore germination of this fruit rot fungus. Further, nucleic acid release, electrical conductivity, and protein and soluble sugar content of the fruit rot fungus gradually increased with increasing eugenol concentrations. Propidium iodide staining revealed that the cell membrane integrity of <i>L. theobromae</i> hyphae was disrupted when treated with eugenol. In addition, eugenol treatment inhibited the spread of disease spot diameter after inoculation with <i>L. theobromae</i>, effectively increased APX, SOD, CAT, POD, 4CL, C4H, and PAL activities, promoted the accumulation of disease-stage-related proteins CHI and GLU, and increased the total phenol and flavonoid content during storage in passion fruit. Overall, these results suggest that eugenol has good application prospects for the effective control of fruit rot in passion fruit. |
| first_indexed | 2024-03-11T04:00:44Z |
| format | Article |
| id | doaj.art-8b2e6c775e6c48d6a4d768f4f31ff54c |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T04:00:44Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-8b2e6c775e6c48d6a4d768f4f31ff54c2023-11-18T00:07:54ZengMDPI AGAgronomy2073-43952023-05-01135140810.3390/agronomy13051408The Inhibitory Mechanism of Eugenol on <i>Lasiodiplodia theobromae</i> and Its Induced Disease Resistance of Passion FruitYanzheng Sun0Liang Shuai1Donglan Luo2Liangjie Ba3Food and Pharmaceutical Engineering Institute, Guiyang University, Guiyang 550005, ChinaSchool of Chemistry and Food Science, Nanchang Normal University, Nanchang 330023, ChinaFood and Pharmaceutical Engineering Institute, Guiyang University, Guiyang 550005, ChinaFood and Pharmaceutical Engineering Institute, Guiyang University, Guiyang 550005, ChinaThe inhibitory effects and mechanisms of eugenol were studied in pathogenic fungi that were isolated from passion fruit affected with naturally occurring fruit rot; additionally, the effect of eugenol treatment on fruit rot resistance in passion fruit was investigated. The corresponding results demonstrated that the pathogenic fungus causing passion fruit rot was <i>Lasiodiplodia theobromae</i> and that the minimal inhibitory concentration of eugenol against <i>L. theobromae</i> was 0.30 mg/mL. Eugenol significantly inhibited mycelial growth and spore germination of this fruit rot fungus. Further, nucleic acid release, electrical conductivity, and protein and soluble sugar content of the fruit rot fungus gradually increased with increasing eugenol concentrations. Propidium iodide staining revealed that the cell membrane integrity of <i>L. theobromae</i> hyphae was disrupted when treated with eugenol. In addition, eugenol treatment inhibited the spread of disease spot diameter after inoculation with <i>L. theobromae</i>, effectively increased APX, SOD, CAT, POD, 4CL, C4H, and PAL activities, promoted the accumulation of disease-stage-related proteins CHI and GLU, and increased the total phenol and flavonoid content during storage in passion fruit. Overall, these results suggest that eugenol has good application prospects for the effective control of fruit rot in passion fruit.https://www.mdpi.com/2073-4395/13/5/1408passion fruiteugenolfruit rotantifungal activitydisease resistance |
| spellingShingle | Yanzheng Sun Liang Shuai Donglan Luo Liangjie Ba The Inhibitory Mechanism of Eugenol on <i>Lasiodiplodia theobromae</i> and Its Induced Disease Resistance of Passion Fruit Agronomy passion fruit eugenol fruit rot antifungal activity disease resistance |
| title | The Inhibitory Mechanism of Eugenol on <i>Lasiodiplodia theobromae</i> and Its Induced Disease Resistance of Passion Fruit |
| title_full | The Inhibitory Mechanism of Eugenol on <i>Lasiodiplodia theobromae</i> and Its Induced Disease Resistance of Passion Fruit |
| title_fullStr | The Inhibitory Mechanism of Eugenol on <i>Lasiodiplodia theobromae</i> and Its Induced Disease Resistance of Passion Fruit |
| title_full_unstemmed | The Inhibitory Mechanism of Eugenol on <i>Lasiodiplodia theobromae</i> and Its Induced Disease Resistance of Passion Fruit |
| title_short | The Inhibitory Mechanism of Eugenol on <i>Lasiodiplodia theobromae</i> and Its Induced Disease Resistance of Passion Fruit |
| title_sort | inhibitory mechanism of eugenol on i lasiodiplodia theobromae i and its induced disease resistance of passion fruit |
| topic | passion fruit eugenol fruit rot antifungal activity disease resistance |
| url | https://www.mdpi.com/2073-4395/13/5/1408 |
| work_keys_str_mv | AT yanzhengsun theinhibitorymechanismofeugenolonilasiodiplodiatheobromaeianditsinduceddiseaseresistanceofpassionfruit AT liangshuai theinhibitorymechanismofeugenolonilasiodiplodiatheobromaeianditsinduceddiseaseresistanceofpassionfruit AT donglanluo theinhibitorymechanismofeugenolonilasiodiplodiatheobromaeianditsinduceddiseaseresistanceofpassionfruit AT liangjieba theinhibitorymechanismofeugenolonilasiodiplodiatheobromaeianditsinduceddiseaseresistanceofpassionfruit AT yanzhengsun inhibitorymechanismofeugenolonilasiodiplodiatheobromaeianditsinduceddiseaseresistanceofpassionfruit AT liangshuai inhibitorymechanismofeugenolonilasiodiplodiatheobromaeianditsinduceddiseaseresistanceofpassionfruit AT donglanluo inhibitorymechanismofeugenolonilasiodiplodiatheobromaeianditsinduceddiseaseresistanceofpassionfruit AT liangjieba inhibitorymechanismofeugenolonilasiodiplodiatheobromaeianditsinduceddiseaseresistanceofpassionfruit |