Isolation and Characterization of Microsatellite Loci for <i>Cornus sanguinea</i> (Cornaceae)
Premise of the study: To facilitate genetic and conservation research of <i>Cornus sanguinea</i>, microsatellite loci were isolated and 29 individuals from 11 German populations were genotyped. Methods and Results: Sixteen microsatellite loci were characterized from an enriched small in...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-09-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1300012 |
| _version_ | 1819116612930764800 |
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| author | Phillip A. Wadl E. Anne Hatmaker Barbara Fussi Brian E. Scheffler Robert N. Trigiano |
| author_facet | Phillip A. Wadl E. Anne Hatmaker Barbara Fussi Brian E. Scheffler Robert N. Trigiano |
| author_sort | Phillip A. Wadl |
| collection | DOAJ |
| description | Premise of the study: To facilitate genetic and conservation research of <i>Cornus sanguinea</i>, microsatellite loci were isolated and 29 individuals from 11 German populations were genotyped.
Methods and Results: Sixteen microsatellite loci were characterized from an enriched small insert genomic library. The number of alleles detected ranged from five to 11 per locus, observed heterozygosity ranged from 0.00 to 1.00, expected heterozygosity ranged from 0.65 to 0.90, and polymorphic information content ranged from 0.59 to 0.88.
Conclusions: The markers described in the study will allow further investigation of population dynamics and the degree of clonal reproduction within populations of <i>C. sanguinea</i>. |
| first_indexed | 2024-12-22T05:19:52Z |
| format | Article |
| id | doaj.art-8b2ee517c9024523807f263a36b38d25 |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-22T05:19:52Z |
| publishDate | 2013-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-8b2ee517c9024523807f263a36b38d252022-12-21T18:37:46ZengWileyApplications in Plant Sciences2168-04502013-09-0119130001210.3732/apps.1300012Isolation and Characterization of Microsatellite Loci for <i>Cornus sanguinea</i> (Cornaceae)Phillip A. Wadl0E. Anne Hatmaker1Barbara Fussi2Brian E. Scheffler3Robert N. Trigiano4Department of Entomology and Plant Pathology, University of Tennessee, 205 Ellington Plant Sciences Building, 2431 Joe Johnson Drive, Knoxville, Tennessee 37996-4560 USADepartment of Entomology and Plant Pathology, University of Tennessee, 205 Ellington Plant Sciences Building, 2431 Joe Johnson Drive, Knoxville, Tennessee 37996-4560 USABavarian Office for Forest Seeding and Planting (ASP), Forstamtsplatz 1, D-83317 Teisendorf, GermanyU.S. Department of Agriculture–Agricultural Research Service (USDA-ARS), Genomics and Bioinformatics Research Unit, 141 Experiment Station Road, P.O. Box 36, Stoneville, Mississippi 38776-0038 USADepartment of Entomology and Plant Pathology, University of Tennessee, 205 Ellington Plant Sciences Building, 2431 Joe Johnson Drive, Knoxville, Tennessee 37996-4560 USAPremise of the study: To facilitate genetic and conservation research of <i>Cornus sanguinea</i>, microsatellite loci were isolated and 29 individuals from 11 German populations were genotyped. Methods and Results: Sixteen microsatellite loci were characterized from an enriched small insert genomic library. The number of alleles detected ranged from five to 11 per locus, observed heterozygosity ranged from 0.00 to 1.00, expected heterozygosity ranged from 0.65 to 0.90, and polymorphic information content ranged from 0.59 to 0.88. Conclusions: The markers described in the study will allow further investigation of population dynamics and the degree of clonal reproduction within populations of <i>C. sanguinea</i>.http://www.bioone.org/doi/full/10.3732/apps.1300012bloodtwig dogwoodCornaceaeCornus sanguineapopulation genetics |
| spellingShingle | Phillip A. Wadl E. Anne Hatmaker Barbara Fussi Brian E. Scheffler Robert N. Trigiano Isolation and Characterization of Microsatellite Loci for <i>Cornus sanguinea</i> (Cornaceae) Applications in Plant Sciences bloodtwig dogwood Cornaceae Cornus sanguinea population genetics |
| title | Isolation and Characterization of Microsatellite Loci for <i>Cornus sanguinea</i> (Cornaceae) |
| title_full | Isolation and Characterization of Microsatellite Loci for <i>Cornus sanguinea</i> (Cornaceae) |
| title_fullStr | Isolation and Characterization of Microsatellite Loci for <i>Cornus sanguinea</i> (Cornaceae) |
| title_full_unstemmed | Isolation and Characterization of Microsatellite Loci for <i>Cornus sanguinea</i> (Cornaceae) |
| title_short | Isolation and Characterization of Microsatellite Loci for <i>Cornus sanguinea</i> (Cornaceae) |
| title_sort | isolation and characterization of microsatellite loci for i cornus sanguinea i cornaceae |
| topic | bloodtwig dogwood Cornaceae Cornus sanguinea population genetics |
| url | http://www.bioone.org/doi/full/10.3732/apps.1300012 |
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