BTH Treatment Delays the Senescence of Postharvest Pitaya Fruit in Relation to Enhancing Antioxidant System and Phenylpropanoid Pathway
The plant resistance elicitor Benzo (1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) can enhance disease resistance of harvested fruit. Nonetheless, it is still unknown whether BTH plays a role in regulating fruit senescence. In this study, exogenous BTH treatment efficiently delayed the...
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MDPI AG
2021-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/4/846 |
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| author | Xiaochun Ding Xiaoyang Zhu Wang Zheng Fengjun Li Shuangling Xiao Xuewu Duan |
| author_facet | Xiaochun Ding Xiaoyang Zhu Wang Zheng Fengjun Li Shuangling Xiao Xuewu Duan |
| author_sort | Xiaochun Ding |
| collection | DOAJ |
| description | The plant resistance elicitor Benzo (1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) can enhance disease resistance of harvested fruit. Nonetheless, it is still unknown whether BTH plays a role in regulating fruit senescence. In this study, exogenous BTH treatment efficiently delayed the senescence of postharvest pitaya fruit with lower lipid peroxidation level. Furthermore, BTH-treated fruit exhibited lower hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) content, higher contents of reduced ascorbic acid (AsA) and reduced glutathione (GSH) levels and higher ratios of reduced to oxidized glutathione (GSH/GSSG) and ascorbic acid (AsA/DHA), as well as higher activities of ROS scavenging enzymes, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD) and glutathione reductase (GR) in comparison with control fruit. Moreover, BTH treatment enhanced the activities of phenylpropanoid pathway-related enzymes, including cinnamate-4-hydroxylase (C4H), phenylalanine ammonia-lyase (PAL) and 4-coumarate/coenzyme A ligase (4CL) and the levels of phenolics, flavonoids and lignin. In addition, BTH treatment upregulated the expression of <i>HuSOD1/3/4</i>, <i>HuCAT2</i>, <i>HuAPX1/2</i> and <i>HuPOD1/2/4</i> genes. These results suggested that application of BTH delayed the senescence of harvested pitaya fruit in relation to enhanced antioxidant system and phenylpropanoid pathway. |
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| last_indexed | 2024-03-10T12:22:19Z |
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| series | Foods |
| spelling | doaj.art-8b31febd586e4bcf9dd3082f682f9fa82023-11-21T15:23:51ZengMDPI AGFoods2304-81582021-04-0110484610.3390/foods10040846BTH Treatment Delays the Senescence of Postharvest Pitaya Fruit in Relation to Enhancing Antioxidant System and Phenylpropanoid PathwayXiaochun Ding0Xiaoyang Zhu1Wang Zheng2Fengjun Li3Shuangling Xiao4Xuewu Duan5South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory for Postharvest Science and Technology of Fruit and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory for Postharvest Science and Technology of Fruit and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaSouth China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Provincial Key Laboratory for Postharvest Science and Technology of Fruit and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaSouth China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaThe plant resistance elicitor Benzo (1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) can enhance disease resistance of harvested fruit. Nonetheless, it is still unknown whether BTH plays a role in regulating fruit senescence. In this study, exogenous BTH treatment efficiently delayed the senescence of postharvest pitaya fruit with lower lipid peroxidation level. Furthermore, BTH-treated fruit exhibited lower hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) content, higher contents of reduced ascorbic acid (AsA) and reduced glutathione (GSH) levels and higher ratios of reduced to oxidized glutathione (GSH/GSSG) and ascorbic acid (AsA/DHA), as well as higher activities of ROS scavenging enzymes, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD) and glutathione reductase (GR) in comparison with control fruit. Moreover, BTH treatment enhanced the activities of phenylpropanoid pathway-related enzymes, including cinnamate-4-hydroxylase (C4H), phenylalanine ammonia-lyase (PAL) and 4-coumarate/coenzyme A ligase (4CL) and the levels of phenolics, flavonoids and lignin. In addition, BTH treatment upregulated the expression of <i>HuSOD1/3/4</i>, <i>HuCAT2</i>, <i>HuAPX1/2</i> and <i>HuPOD1/2/4</i> genes. These results suggested that application of BTH delayed the senescence of harvested pitaya fruit in relation to enhanced antioxidant system and phenylpropanoid pathway.https://www.mdpi.com/2304-8158/10/4/846pitayaBTHROS metabolismphenylpropanoid pathway |
| spellingShingle | Xiaochun Ding Xiaoyang Zhu Wang Zheng Fengjun Li Shuangling Xiao Xuewu Duan BTH Treatment Delays the Senescence of Postharvest Pitaya Fruit in Relation to Enhancing Antioxidant System and Phenylpropanoid Pathway Foods pitaya BTH ROS metabolism phenylpropanoid pathway |
| title | BTH Treatment Delays the Senescence of Postharvest Pitaya Fruit in Relation to Enhancing Antioxidant System and Phenylpropanoid Pathway |
| title_full | BTH Treatment Delays the Senescence of Postharvest Pitaya Fruit in Relation to Enhancing Antioxidant System and Phenylpropanoid Pathway |
| title_fullStr | BTH Treatment Delays the Senescence of Postharvest Pitaya Fruit in Relation to Enhancing Antioxidant System and Phenylpropanoid Pathway |
| title_full_unstemmed | BTH Treatment Delays the Senescence of Postharvest Pitaya Fruit in Relation to Enhancing Antioxidant System and Phenylpropanoid Pathway |
| title_short | BTH Treatment Delays the Senescence of Postharvest Pitaya Fruit in Relation to Enhancing Antioxidant System and Phenylpropanoid Pathway |
| title_sort | bth treatment delays the senescence of postharvest pitaya fruit in relation to enhancing antioxidant system and phenylpropanoid pathway |
| topic | pitaya BTH ROS metabolism phenylpropanoid pathway |
| url | https://www.mdpi.com/2304-8158/10/4/846 |
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