The protective effect of Luteolin on chicken spleen lymphocytes from ammonia poisoning through mitochondria and balancing energy metabolism disorders
ABSTRACT: Ammonia poses a significant challenge in the contemporary intensive breeding industry, resulting in substantial economic losses. Despite this, there is a dearth of research investigating efficacious strategies to prevent ammonia poisoning in poultry. Consequently, the objective of this stu...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123006120 |
| _version_ | 1827593733469634560 |
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| author | Dechun Chen Fanyu Shen Jiahao Liu Haojinming Tang Kai Zhang Xiaohua Teng Falong Yang |
| author_facet | Dechun Chen Fanyu Shen Jiahao Liu Haojinming Tang Kai Zhang Xiaohua Teng Falong Yang |
| author_sort | Dechun Chen |
| collection | DOAJ |
| description | ABSTRACT: Ammonia poses a significant challenge in the contemporary intensive breeding industry, resulting in substantial economic losses. Despite this, there is a dearth of research investigating efficacious strategies to prevent ammonia poisoning in poultry. Consequently, the objective of this study was to investigate the molecular mechanisms through which Luteolin (Lut) safeguards mitochondria and restores equilibrium to energy metabolism disorders, thereby shielding chicken spleen lymphocytes from the detrimental effects of ammonia poisoning. Chicken spleen lymphocytes were categorized into 3 distinct groups: the control group, the ammonia group (with the addition of 1 mmol/L of ammonium chloride), and the Lut group (with the treatment of 0.5 μg/mL of Lut for 12 h followed by the addition of 1 mmol/L of ammonium chloride). These groups were then cultured for a duration of 24 h. To investigate the potential protective effect of Lut on lymphocytes exposed to ammonia, various techniques were employed, including CCK-8 analysis, ultrastructural observation, reagent kit methodology, fluorescence microscopy, and quantitative real-time PCR (qRT-PCR). The findings indicate that Lut has the potential to mitigate the morphological damage of mitochondria caused by ammonia poisoning. Additionally, it can counteract the decline in mitochondrial membrane potential, ATP content, and ATPase activities (specifically Na+/K+-ATPase, Ca2+-ATPase, Mg2+-ATPase, and Ca/Mg2+-ATPase) following exposure to ammonia in lymphocytes. Lut also has the ability to regulate the expression of genes involved in mitochondrial fusion (Opa1, Mfn1, and Mfn2) and division (Drp1 and Mff) in spleen lymphocytes after ammonia exposure. This regulation leads to a balanced energy metabolism (HK1, HK2, LDHA, LDHB, PFK, PK, SDHB, and ACO2) and provides protection against ammonia poisoning. |
| first_indexed | 2024-03-09T02:15:58Z |
| format | Article |
| id | doaj.art-8b3409f9b3bd40a5afcf46077954c97d |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-09T02:15:58Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-8b3409f9b3bd40a5afcf46077954c97d2023-12-07T05:27:10ZengElsevierPoultry Science0032-57912023-12-0110212103093The protective effect of Luteolin on chicken spleen lymphocytes from ammonia poisoning through mitochondria and balancing energy metabolism disordersDechun Chen0Fanyu Shen1Jiahao Liu2Haojinming Tang3Kai Zhang4Xiaohua Teng5Falong Yang6Southwest Minzu University Key Laboratory of Animal Medicine in Sichuan Province, Southwest Minzu University, Chengdu 610041, ChinaSouthwest Minzu University Key Laboratory of Animal Medicine in Sichuan Province, Southwest Minzu University, Chengdu 610041, ChinaSouthwest Minzu University Key Laboratory of Animal Medicine in Sichuan Province, Southwest Minzu University, Chengdu 610041, ChinaSouthwest Minzu University Key Laboratory of Animal Medicine in Sichuan Province, Southwest Minzu University, Chengdu 610041, ChinaSouthwest Minzu University Key Laboratory of Animal Medicine in Sichuan Province, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaSouthwest Minzu University Key Laboratory of Animal Medicine in Sichuan Province, Southwest Minzu University, Chengdu 610041, China; Corresponding author:ABSTRACT: Ammonia poses a significant challenge in the contemporary intensive breeding industry, resulting in substantial economic losses. Despite this, there is a dearth of research investigating efficacious strategies to prevent ammonia poisoning in poultry. Consequently, the objective of this study was to investigate the molecular mechanisms through which Luteolin (Lut) safeguards mitochondria and restores equilibrium to energy metabolism disorders, thereby shielding chicken spleen lymphocytes from the detrimental effects of ammonia poisoning. Chicken spleen lymphocytes were categorized into 3 distinct groups: the control group, the ammonia group (with the addition of 1 mmol/L of ammonium chloride), and the Lut group (with the treatment of 0.5 μg/mL of Lut for 12 h followed by the addition of 1 mmol/L of ammonium chloride). These groups were then cultured for a duration of 24 h. To investigate the potential protective effect of Lut on lymphocytes exposed to ammonia, various techniques were employed, including CCK-8 analysis, ultrastructural observation, reagent kit methodology, fluorescence microscopy, and quantitative real-time PCR (qRT-PCR). The findings indicate that Lut has the potential to mitigate the morphological damage of mitochondria caused by ammonia poisoning. Additionally, it can counteract the decline in mitochondrial membrane potential, ATP content, and ATPase activities (specifically Na+/K+-ATPase, Ca2+-ATPase, Mg2+-ATPase, and Ca/Mg2+-ATPase) following exposure to ammonia in lymphocytes. Lut also has the ability to regulate the expression of genes involved in mitochondrial fusion (Opa1, Mfn1, and Mfn2) and division (Drp1 and Mff) in spleen lymphocytes after ammonia exposure. This regulation leads to a balanced energy metabolism (HK1, HK2, LDHA, LDHB, PFK, PK, SDHB, and ACO2) and provides protection against ammonia poisoning.http://www.sciencedirect.com/science/article/pii/S0032579123006120ammoniachickenLuteolinmitochondriaenergy metabolism |
| spellingShingle | Dechun Chen Fanyu Shen Jiahao Liu Haojinming Tang Kai Zhang Xiaohua Teng Falong Yang The protective effect of Luteolin on chicken spleen lymphocytes from ammonia poisoning through mitochondria and balancing energy metabolism disorders Poultry Science ammonia chicken Luteolin mitochondria energy metabolism |
| title | The protective effect of Luteolin on chicken spleen lymphocytes from ammonia poisoning through mitochondria and balancing energy metabolism disorders |
| title_full | The protective effect of Luteolin on chicken spleen lymphocytes from ammonia poisoning through mitochondria and balancing energy metabolism disorders |
| title_fullStr | The protective effect of Luteolin on chicken spleen lymphocytes from ammonia poisoning through mitochondria and balancing energy metabolism disorders |
| title_full_unstemmed | The protective effect of Luteolin on chicken spleen lymphocytes from ammonia poisoning through mitochondria and balancing energy metabolism disorders |
| title_short | The protective effect of Luteolin on chicken spleen lymphocytes from ammonia poisoning through mitochondria and balancing energy metabolism disorders |
| title_sort | protective effect of luteolin on chicken spleen lymphocytes from ammonia poisoning through mitochondria and balancing energy metabolism disorders |
| topic | ammonia chicken Luteolin mitochondria energy metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123006120 |
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