Very low density lipoproteins and lipoprotein lipase in serum of rats deficient in essential fatty acids
Rats fed a diet deficient in essential fatty acids have a low level of serum very low density lipoproteins (VLDL). It was found that after intraperitoneal injection of heparin, deficient rats had a higher level of lipoprotein lipase activity in their plasma than did normal rats. VLDL isolated from s...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
1972-03-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520394220 |
| _version_ | 1819076233085845504 |
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| author | G.G. de Pury F.D. Collins |
| author_facet | G.G. de Pury F.D. Collins |
| author_sort | G.G. de Pury |
| collection | DOAJ |
| description | Rats fed a diet deficient in essential fatty acids have a low level of serum very low density lipoproteins (VLDL). It was found that after intraperitoneal injection of heparin, deficient rats had a higher level of lipoprotein lipase activity in their plasma than did normal rats. VLDL isolated from serum of normal and deficient rats were compared as substrates for postheparin lipase of rat plasma. There was no significant difference in Vmax between the two preparations of lipoproteins, but the apparent Km for lipoproteins from deficient animals was significantly less than that for normal animals. These observations suggest that the low concentration of VLDL in deficient rats may be explained (a) by an increased activity of lipoprotein lipase in the tissues of these animals and (b) by the VLDL of deficient rats being more rapidly hydrolyzed at low concentrations by lipoprotein lipase than VLDL from normal rats. |
| first_indexed | 2024-12-21T18:38:03Z |
| format | Article |
| id | doaj.art-8b340ab2dc54415f994cc0d646ec755c |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T18:38:03Z |
| publishDate | 1972-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-8b340ab2dc54415f994cc0d646ec755c2022-12-21T18:54:06ZengElsevierJournal of Lipid Research0022-22751972-03-01132268275Very low density lipoproteins and lipoprotein lipase in serum of rats deficient in essential fatty acidsG.G. de Pury0F.D. Collins1Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria 3052, AustraliaRussell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria 3052, AustraliaRats fed a diet deficient in essential fatty acids have a low level of serum very low density lipoproteins (VLDL). It was found that after intraperitoneal injection of heparin, deficient rats had a higher level of lipoprotein lipase activity in their plasma than did normal rats. VLDL isolated from serum of normal and deficient rats were compared as substrates for postheparin lipase of rat plasma. There was no significant difference in Vmax between the two preparations of lipoproteins, but the apparent Km for lipoproteins from deficient animals was significantly less than that for normal animals. These observations suggest that the low concentration of VLDL in deficient rats may be explained (a) by an increased activity of lipoprotein lipase in the tissues of these animals and (b) by the VLDL of deficient rats being more rapidly hydrolyzed at low concentrations by lipoprotein lipase than VLDL from normal rats.http://www.sciencedirect.com/science/article/pii/S0022227520394220fatty acid compositionphospholipidstriglyceridescholesteryl estersheparinenzyme kinetics |
| spellingShingle | G.G. de Pury F.D. Collins Very low density lipoproteins and lipoprotein lipase in serum of rats deficient in essential fatty acids Journal of Lipid Research fatty acid composition phospholipids triglycerides cholesteryl esters heparin enzyme kinetics |
| title | Very low density lipoproteins and lipoprotein lipase in serum of rats deficient in essential fatty acids |
| title_full | Very low density lipoproteins and lipoprotein lipase in serum of rats deficient in essential fatty acids |
| title_fullStr | Very low density lipoproteins and lipoprotein lipase in serum of rats deficient in essential fatty acids |
| title_full_unstemmed | Very low density lipoproteins and lipoprotein lipase in serum of rats deficient in essential fatty acids |
| title_short | Very low density lipoproteins and lipoprotein lipase in serum of rats deficient in essential fatty acids |
| title_sort | very low density lipoproteins and lipoprotein lipase in serum of rats deficient in essential fatty acids |
| topic | fatty acid composition phospholipids triglycerides cholesteryl esters heparin enzyme kinetics |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520394220 |
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