Filling nanopipettes with apertures smaller than 50 nm: dynamic microdistillation
Using nanopipettes with very small apertures (<10 nm) is a good way to improve the spatial resolution in scanning conductance experiments, to monitor single-molecule delivery and to strain long molecules stretching during translocation. However, such nanopipettes can be difficult to fill. Here we...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Beilstein-Institut
2018-08-01
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| Series: | Beilstein Journal of Nanotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.3762/bjnano.9.204 |
| _version_ | 1819265115899297792 |
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| author | Evelyne Salançon Bernard Tinland |
| author_facet | Evelyne Salançon Bernard Tinland |
| author_sort | Evelyne Salançon |
| collection | DOAJ |
| description | Using nanopipettes with very small apertures (<10 nm) is a good way to improve the spatial resolution in scanning conductance experiments, to monitor single-molecule delivery and to strain long molecules stretching during translocation. However, such nanopipettes can be difficult to fill. Here we describe a dynamic microdistillation technique that successfully fills all nanopipettes, whatever their shape or tip radius. Even elongated or bent nanopipettes with a small-angle tip are completely filled using this new technique. The nanopipettes are first filled with pure water, which is later replaced with the desired electrolyte via electromigration. Electrical measurements are used to check that filling is complete. |
| first_indexed | 2024-12-23T20:40:15Z |
| format | Article |
| id | doaj.art-8b34eef93af84bd7bb9503b5c144346a |
| institution | Directory Open Access Journal |
| issn | 2190-4286 |
| language | English |
| last_indexed | 2024-12-23T20:40:15Z |
| publishDate | 2018-08-01 |
| publisher | Beilstein-Institut |
| record_format | Article |
| series | Beilstein Journal of Nanotechnology |
| spelling | doaj.art-8b34eef93af84bd7bb9503b5c144346a2022-12-21T17:31:56ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862018-08-01912181218710.3762/bjnano.9.2042190-4286-9-204Filling nanopipettes with apertures smaller than 50 nm: dynamic microdistillationEvelyne Salançon0Bernard Tinland1Aix Marseille Univ, CNRS, CINAM, Marseille, FranceAix Marseille Univ, CNRS, CINAM, Marseille, FranceUsing nanopipettes with very small apertures (<10 nm) is a good way to improve the spatial resolution in scanning conductance experiments, to monitor single-molecule delivery and to strain long molecules stretching during translocation. However, such nanopipettes can be difficult to fill. Here we describe a dynamic microdistillation technique that successfully fills all nanopipettes, whatever their shape or tip radius. Even elongated or bent nanopipettes with a small-angle tip are completely filled using this new technique. The nanopipettes are first filled with pure water, which is later replaced with the desired electrolyte via electromigration. Electrical measurements are used to check that filling is complete.https://doi.org/10.3762/bjnano.9.204current rectificationdistillationfillingnanopipette |
| spellingShingle | Evelyne Salançon Bernard Tinland Filling nanopipettes with apertures smaller than 50 nm: dynamic microdistillation Beilstein Journal of Nanotechnology current rectification distillation filling nanopipette |
| title | Filling nanopipettes with apertures smaller than 50 nm: dynamic microdistillation |
| title_full | Filling nanopipettes with apertures smaller than 50 nm: dynamic microdistillation |
| title_fullStr | Filling nanopipettes with apertures smaller than 50 nm: dynamic microdistillation |
| title_full_unstemmed | Filling nanopipettes with apertures smaller than 50 nm: dynamic microdistillation |
| title_short | Filling nanopipettes with apertures smaller than 50 nm: dynamic microdistillation |
| title_sort | filling nanopipettes with apertures smaller than 50 nm dynamic microdistillation |
| topic | current rectification distillation filling nanopipette |
| url | https://doi.org/10.3762/bjnano.9.204 |
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