Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate
Abstract Background Butyl acetate is a versatile compound that is widely used in the chemical and food industry. The conventional butyl acetate synthesis via Fischer esterification of butanol and acetic acid using catalytic strong acids under high temperature is not environmentally benign. Alternati...
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| Format: | Article |
| Language: | English |
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BMC
2022-02-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-022-01755-y |
| _version_ | 1818501278144135168 |
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| author | Jason T. Ku Arvin Y. Chen Ethan I. Lan |
| author_facet | Jason T. Ku Arvin Y. Chen Ethan I. Lan |
| author_sort | Jason T. Ku |
| collection | DOAJ |
| description | Abstract Background Butyl acetate is a versatile compound that is widely used in the chemical and food industry. The conventional butyl acetate synthesis via Fischer esterification of butanol and acetic acid using catalytic strong acids under high temperature is not environmentally benign. Alternative lipase-catalyzed ester formation requires a significant amount of organic solvent which also presents another environmental challenge. Therefore, a microbial cell factory capable of producing butyl acetate through fermentation of renewable resources would provide a greener approach to butyl acetate production. Result Here, we developed a metabolically engineered strain of Escherichia coli that efficiently converts glucose to butyl acetate. A modified Clostridium CoA-dependent butanol production pathway was used to synthesize butanol which was then condensed with acetyl-CoA through an alcohol acetyltransferase. Optimization of alcohol acetyltransferase expression and redox balance with auto-inducible fermentative controlled gene expression led to an effective titer of 22.8 ± 1.8 g/L butyl acetate produced in a bench-top bioreactor. Conclusion Building on the well-developed Clostridium CoA-dependent butanol biosynthetic pathway, expression of an alcohol acetyltransferase converts the butanol produced into butyl acetate. The results from this study provided a strain of E. coli capable of directly producing butyl acetate from renewable resources at ambient conditions. |
| first_indexed | 2024-12-10T20:53:58Z |
| format | Article |
| id | doaj.art-8b350c6e496a48b2aeff4691601e098e |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-10T20:53:58Z |
| publishDate | 2022-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-8b350c6e496a48b2aeff4691601e098e2022-12-22T01:34:00ZengBMCMicrobial Cell Factories1475-28592022-02-0121111110.1186/s12934-022-01755-yMetabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetateJason T. Ku0Arvin Y. Chen1Ethan I. Lan2Institute of Molecular Medicine and Bioengineering, National Chiao Tung UniversityInstitute of Molecular Medicine and Bioengineering, National Chiao Tung UniversityDepartment of Biological Science and Technology, National Chiao Tung UniversityAbstract Background Butyl acetate is a versatile compound that is widely used in the chemical and food industry. The conventional butyl acetate synthesis via Fischer esterification of butanol and acetic acid using catalytic strong acids under high temperature is not environmentally benign. Alternative lipase-catalyzed ester formation requires a significant amount of organic solvent which also presents another environmental challenge. Therefore, a microbial cell factory capable of producing butyl acetate through fermentation of renewable resources would provide a greener approach to butyl acetate production. Result Here, we developed a metabolically engineered strain of Escherichia coli that efficiently converts glucose to butyl acetate. A modified Clostridium CoA-dependent butanol production pathway was used to synthesize butanol which was then condensed with acetyl-CoA through an alcohol acetyltransferase. Optimization of alcohol acetyltransferase expression and redox balance with auto-inducible fermentative controlled gene expression led to an effective titer of 22.8 ± 1.8 g/L butyl acetate produced in a bench-top bioreactor. Conclusion Building on the well-developed Clostridium CoA-dependent butanol biosynthetic pathway, expression of an alcohol acetyltransferase converts the butanol produced into butyl acetate. The results from this study provided a strain of E. coli capable of directly producing butyl acetate from renewable resources at ambient conditions.https://doi.org/10.1186/s12934-022-01755-yAlcohol acetyltransferaseATF1Butyl acetateButanolEsterMetabolic engineering |
| spellingShingle | Jason T. Ku Arvin Y. Chen Ethan I. Lan Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate Microbial Cell Factories Alcohol acetyltransferase ATF1 Butyl acetate Butanol Ester Metabolic engineering |
| title | Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate |
| title_full | Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate |
| title_fullStr | Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate |
| title_full_unstemmed | Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate |
| title_short | Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate |
| title_sort | metabolic engineering of escherichia coli for efficient biosynthesis of butyl acetate |
| topic | Alcohol acetyltransferase ATF1 Butyl acetate Butanol Ester Metabolic engineering |
| url | https://doi.org/10.1186/s12934-022-01755-y |
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