Construction of keratin 5-IRES-eGFP knockin hESCs cell line

Construction of keratin 5-IRES-eGFP knockin hESCs cell line

Objective To establish keratin 5-IRES-eGFP knockin hESCs cell line by using CRISPR/Cas9 technique, which could provide a cell model for the study of skin diseases with abnormal basal keratinocytes in the epidermis. Methods The sgRNA plasmid and target Donor plasmid targeting gene keratin 5 were cons...

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Bibliographic Details
Main Authors: Wen ZHENG, Yadan ZHONG, Xuan WANG, Huiting LIU, Bin YANG, Jun LIU
Format: Article
Language:zho
Published: editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology 2022-08-01
Series:Pifu-xingbing zhenliaoxue zazhi
Subjects:
human embryonic stem cell
crispr/cas9
keratin 5
basal keratinocytes
Online Access:http://pfxbzlx.gdvdc.com/CN/abstract/abstract2091.shtml

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http://pfxbzlx.gdvdc.com/CN/abstract/abstract2091.shtml

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