Rapid Detection of Deoxynivalenol in Dry Pasta Using a Label-Free Immunosensor
This work focused on the development and optimization of an impedimetric label-free immunosensor for detecting deoxynivalenol (DON). A monoclonal antibody for DON detection was immobilized on a modified gold electrode with a cysteamine layer and polyamidoamine (PAMAM) dendrimers. Cyclic voltammetry...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/4/240 |
| _version_ | 1797436695002480640 |
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| author | Francesca Malvano Roberto Pilloton Alfredo Rubino Donatella Albanese |
| author_facet | Francesca Malvano Roberto Pilloton Alfredo Rubino Donatella Albanese |
| author_sort | Francesca Malvano |
| collection | DOAJ |
| description | This work focused on the development and optimization of an impedimetric label-free immunosensor for detecting deoxynivalenol (DON). A monoclonal antibody for DON detection was immobilized on a modified gold electrode with a cysteamine layer and polyamidoamine (PAMAM) dendrimers. Cyclic voltammetry and electrochemical impedance spectroscopy techniques were used to monitor the layer-by-layer development of the immunosensor design, while electrochemical impedance spectroscopy and differential pulse voltammetry were employed to investigate the antigen/antibody interaction. The PAMAM dendrimers, allowing to immobilize a large number of monoclonal antibodies, permitted reaching, through the DPV technique, a high sensitivity and a low limit of detection equal to 1 ppb. The evaluation of the possible reuse of the immunosensors highlighted a decrease in the analytical performances of the regenerated immunosensors. After evaluating the matrix effect, the developed immunosensor was used to quantify DON in pasta samples spiked with a known mycotoxin concentration. Taking into consideration the DON extraction procedure used for the pasta samples and the matrix effect related to the sample, the proposed immunosensor showed a limit of detection of 50 ppb, which is lower than the maximum residual limit imposed by European Regulation for DON in dry pasta (750 ppb). |
| first_indexed | 2024-03-09T11:05:16Z |
| format | Article |
| id | doaj.art-8b3a77cf50264b16a49070a4d4a52e71 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T11:05:16Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-8b3a77cf50264b16a49070a4d4a52e712023-12-01T00:58:25ZengMDPI AGBiosensors2079-63742022-04-0112424010.3390/bios12040240Rapid Detection of Deoxynivalenol in Dry Pasta Using a Label-Free ImmunosensorFrancesca Malvano0Roberto Pilloton1Alfredo Rubino2Donatella Albanese3Department of Industrial Engineering, University of Salerno, 84084 Fisciano, SA, ItalyInstitute of Crystallography of the National Research Council (CNR), Department of Chemistry and Material Technology, 00015 Montelibretti, RA, ItalyDepartment of Industrial Engineering, University of Salerno, 84084 Fisciano, SA, ItalyDepartment of Industrial Engineering, University of Salerno, 84084 Fisciano, SA, ItalyThis work focused on the development and optimization of an impedimetric label-free immunosensor for detecting deoxynivalenol (DON). A monoclonal antibody for DON detection was immobilized on a modified gold electrode with a cysteamine layer and polyamidoamine (PAMAM) dendrimers. Cyclic voltammetry and electrochemical impedance spectroscopy techniques were used to monitor the layer-by-layer development of the immunosensor design, while electrochemical impedance spectroscopy and differential pulse voltammetry were employed to investigate the antigen/antibody interaction. The PAMAM dendrimers, allowing to immobilize a large number of monoclonal antibodies, permitted reaching, through the DPV technique, a high sensitivity and a low limit of detection equal to 1 ppb. The evaluation of the possible reuse of the immunosensors highlighted a decrease in the analytical performances of the regenerated immunosensors. After evaluating the matrix effect, the developed immunosensor was used to quantify DON in pasta samples spiked with a known mycotoxin concentration. Taking into consideration the DON extraction procedure used for the pasta samples and the matrix effect related to the sample, the proposed immunosensor showed a limit of detection of 50 ppb, which is lower than the maximum residual limit imposed by European Regulation for DON in dry pasta (750 ppb).https://www.mdpi.com/2079-6374/12/4/240biosensorsantibodymycotoxindifferential pulse voltammetrygold electrodePAMAM |
| spellingShingle | Francesca Malvano Roberto Pilloton Alfredo Rubino Donatella Albanese Rapid Detection of Deoxynivalenol in Dry Pasta Using a Label-Free Immunosensor Biosensors biosensors antibody mycotoxin differential pulse voltammetry gold electrode PAMAM |
| title | Rapid Detection of Deoxynivalenol in Dry Pasta Using a Label-Free Immunosensor |
| title_full | Rapid Detection of Deoxynivalenol in Dry Pasta Using a Label-Free Immunosensor |
| title_fullStr | Rapid Detection of Deoxynivalenol in Dry Pasta Using a Label-Free Immunosensor |
| title_full_unstemmed | Rapid Detection of Deoxynivalenol in Dry Pasta Using a Label-Free Immunosensor |
| title_short | Rapid Detection of Deoxynivalenol in Dry Pasta Using a Label-Free Immunosensor |
| title_sort | rapid detection of deoxynivalenol in dry pasta using a label free immunosensor |
| topic | biosensors antibody mycotoxin differential pulse voltammetry gold electrode PAMAM |
| url | https://www.mdpi.com/2079-6374/12/4/240 |
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