Antioxidant and Antimicrobial Activity of <em>Cleome</em> <em>droserifolia</em> (Forssk.) Del. and Its Biological Effects on Redox Status, Immunity, and Gut Microflora
This study aimed to investigate the antioxidant, antimicrobial, and immunomodulatory activities of a <i>Cleome droserifolia</i> (Forssk.) Del. (Cd) shoot methanolic extracts considering the biological activity of its phytogenic compounds. For this purpose, the Cd phenolic compounds were...
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MDPI AG
2021-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/7/1929 |
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| author | Nesrein M. Hashem Mohamed G. Shehata |
| author_facet | Nesrein M. Hashem Mohamed G. Shehata |
| author_sort | Nesrein M. Hashem |
| collection | DOAJ |
| description | This study aimed to investigate the antioxidant, antimicrobial, and immunomodulatory activities of a <i>Cleome droserifolia</i> (Forssk.) Del. (Cd) shoot methanolic extracts considering the biological activity of its phytogenic compounds. For this purpose, the Cd phenolic compounds were detected, and an in vitro evaluation of the antioxidant and antimicrobial activities of the Cd extract was performed. For a biological evaluation, 30 v-line rabbits were randomly distributed into three groups with treatments including: a basal diet without Cd shoots powder supplement (C group) or supplemented with 1.25- (Cdl group) or 2.5 (Cdh group)-mg Cd/kg dry matter (DM). The Cd extract showed a linear scavenging activity for 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), with the maximal activity observed at a concentration of 1 mg/mL. A total of 16 phenolic compounds were identified by reverse-phase high-performance liquid chromatography (RP-HPLC) in the Cd methanolic extract, among which benzoic acid, rutin, ellagic acid, <i>naringenin</i>, and o-coumaric acid were the major compounds. The methanolic extract of Cd showed inhibitory actions against microbial pathogen species. The in vivo study showed that the two concentrations of Cd significantly improved the redox status of the blood plasma and lysozyme activity. Treatment with Cdh significantly decreased the levels of interleukin-β1 in the blood plasma compared with the control. Moreover, the two concentrations of Cd significantly increased the counts of intestinal and cecal yeast and <i>Lactobacillus</i> species and decreased the <i>Salmonella</i> and <i>Coliform</i> species compared with the control. The aerial parts of the Cd shrub had strong antioxidant, antimicrobial, and immunomodulatory activities, which can improve the overall health status and seem to be related to its impressive range of biologically active phenolic compounds. |
| first_indexed | 2024-03-10T09:58:49Z |
| format | Article |
| id | doaj.art-8b3a9820847445dcb2a8a011e7341722 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T09:58:49Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-8b3a9820847445dcb2a8a011e73417222023-11-22T02:05:10ZengMDPI AGAnimals2076-26152021-06-01117192910.3390/ani11071929Antioxidant and Antimicrobial Activity of <em>Cleome</em> <em>droserifolia</em> (Forssk.) Del. and Its Biological Effects on Redox Status, Immunity, and Gut MicrofloraNesrein M. Hashem0Mohamed G. Shehata1Department of Animal and Fish Production, Faculty of Agriculture, Alexandria University, Alexandria 21545, EgyptDepartment of Food Technology, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications (SRTACITY), New Borg El Arab, Alexandria 21545, EgyptThis study aimed to investigate the antioxidant, antimicrobial, and immunomodulatory activities of a <i>Cleome droserifolia</i> (Forssk.) Del. (Cd) shoot methanolic extracts considering the biological activity of its phytogenic compounds. For this purpose, the Cd phenolic compounds were detected, and an in vitro evaluation of the antioxidant and antimicrobial activities of the Cd extract was performed. For a biological evaluation, 30 v-line rabbits were randomly distributed into three groups with treatments including: a basal diet without Cd shoots powder supplement (C group) or supplemented with 1.25- (Cdl group) or 2.5 (Cdh group)-mg Cd/kg dry matter (DM). The Cd extract showed a linear scavenging activity for 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), with the maximal activity observed at a concentration of 1 mg/mL. A total of 16 phenolic compounds were identified by reverse-phase high-performance liquid chromatography (RP-HPLC) in the Cd methanolic extract, among which benzoic acid, rutin, ellagic acid, <i>naringenin</i>, and o-coumaric acid were the major compounds. The methanolic extract of Cd showed inhibitory actions against microbial pathogen species. The in vivo study showed that the two concentrations of Cd significantly improved the redox status of the blood plasma and lysozyme activity. Treatment with Cdh significantly decreased the levels of interleukin-β1 in the blood plasma compared with the control. Moreover, the two concentrations of Cd significantly increased the counts of intestinal and cecal yeast and <i>Lactobacillus</i> species and decreased the <i>Salmonella</i> and <i>Coliform</i> species compared with the control. The aerial parts of the Cd shrub had strong antioxidant, antimicrobial, and immunomodulatory activities, which can improve the overall health status and seem to be related to its impressive range of biologically active phenolic compounds.https://www.mdpi.com/2076-2615/11/7/1929phytogenicphenolsantioxidantantimicrobial activityimmunity |
| spellingShingle | Nesrein M. Hashem Mohamed G. Shehata Antioxidant and Antimicrobial Activity of <em>Cleome</em> <em>droserifolia</em> (Forssk.) Del. and Its Biological Effects on Redox Status, Immunity, and Gut Microflora Animals phytogenic phenols antioxidant antimicrobial activity immunity |
| title | Antioxidant and Antimicrobial Activity of <em>Cleome</em> <em>droserifolia</em> (Forssk.) Del. and Its Biological Effects on Redox Status, Immunity, and Gut Microflora |
| title_full | Antioxidant and Antimicrobial Activity of <em>Cleome</em> <em>droserifolia</em> (Forssk.) Del. and Its Biological Effects on Redox Status, Immunity, and Gut Microflora |
| title_fullStr | Antioxidant and Antimicrobial Activity of <em>Cleome</em> <em>droserifolia</em> (Forssk.) Del. and Its Biological Effects on Redox Status, Immunity, and Gut Microflora |
| title_full_unstemmed | Antioxidant and Antimicrobial Activity of <em>Cleome</em> <em>droserifolia</em> (Forssk.) Del. and Its Biological Effects on Redox Status, Immunity, and Gut Microflora |
| title_short | Antioxidant and Antimicrobial Activity of <em>Cleome</em> <em>droserifolia</em> (Forssk.) Del. and Its Biological Effects on Redox Status, Immunity, and Gut Microflora |
| title_sort | antioxidant and antimicrobial activity of em cleome em em droserifolia em forssk del and its biological effects on redox status immunity and gut microflora |
| topic | phytogenic phenols antioxidant antimicrobial activity immunity |
| url | https://www.mdpi.com/2076-2615/11/7/1929 |
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