Isolation and Identification of Methylotrophic Bacteria Producing Methanol Dehydrogenase from Human Feet and Mouth
The human feet and mouth are known as sources of methylated sulfides, which are produced by other microflora. Methylated sulfides could be oxidized by methylotrophic bacteria, which may result in odor reduction in human feet and mouth. In this study, we collected a total of 21 isolates from human fe...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2011-03-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1978301916301681 |
| _version_ | 1828531511254581248 |
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| author | DIANA ELIZABETH WATURANGI CATHERINE DELANY NICHOLAS CINDY OKTAVIA SUSANTO MAGGY THENAWIJAYA SUHARTONO |
| author_facet | DIANA ELIZABETH WATURANGI CATHERINE DELANY NICHOLAS CINDY OKTAVIA SUSANTO MAGGY THENAWIJAYA SUHARTONO |
| author_sort | DIANA ELIZABETH WATURANGI |
| collection | DOAJ |
| description | The human feet and mouth are known as sources of methylated sulfides, which are produced by other microflora. Methylated sulfides could be oxidized by methylotrophic bacteria, which may result in odor reduction in human feet and mouth. In this study, we collected a total of 21 isolates from human feet, and 37 isolates from human mouth. These isolates were identified with biochemical test such as oxidase and catalase test and Gram staining assay. The presence of mxaF gene of methanol dehydrogenase was detected by PCR using specific primers. However, the result showed that most of the isolates did not possess mxaF gene. Hence, the methanol dehydrogenase (MDH) activity was also determined. From the total 21 isolates obtained from the feet, only 15 of them showed MDH activity whereas 23 isolates from the total 37 isolates obtained from teeth and tongue region also showed MDH activity. Isolate K25-3 (74.444 U/ml), K33-6 (79.815 U/ml), and K43-5 (69.259 U/ml) from human feet and M41L3 (135.926 U/ml), M27G2 (85.556 U/ml), and M51G1 (103.333 U/ml) from human mouth showed the highest total enzyme activity. Isolates with the highest total activity could be used for further studies such as purification of the enzyme and isolates characterization. |
| first_indexed | 2024-12-11T22:38:46Z |
| format | Article |
| id | doaj.art-8b3ba62a95ff40f5a14f88d2b1e38949 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 |
| language | English |
| last_indexed | 2024-12-11T22:38:46Z |
| publishDate | 2011-03-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-8b3ba62a95ff40f5a14f88d2b1e389492022-12-22T00:47:52ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192011-03-01181111510.4308/hjb.18.1.11Isolation and Identification of Methylotrophic Bacteria Producing Methanol Dehydrogenase from Human Feet and MouthDIANA ELIZABETH WATURANGICATHERINE DELANY NICHOLASCINDY OKTAVIA SUSANTOMAGGY THENAWIJAYA SUHARTONOThe human feet and mouth are known as sources of methylated sulfides, which are produced by other microflora. Methylated sulfides could be oxidized by methylotrophic bacteria, which may result in odor reduction in human feet and mouth. In this study, we collected a total of 21 isolates from human feet, and 37 isolates from human mouth. These isolates were identified with biochemical test such as oxidase and catalase test and Gram staining assay. The presence of mxaF gene of methanol dehydrogenase was detected by PCR using specific primers. However, the result showed that most of the isolates did not possess mxaF gene. Hence, the methanol dehydrogenase (MDH) activity was also determined. From the total 21 isolates obtained from the feet, only 15 of them showed MDH activity whereas 23 isolates from the total 37 isolates obtained from teeth and tongue region also showed MDH activity. Isolate K25-3 (74.444 U/ml), K33-6 (79.815 U/ml), and K43-5 (69.259 U/ml) from human feet and M41L3 (135.926 U/ml), M27G2 (85.556 U/ml), and M51G1 (103.333 U/ml) from human mouth showed the highest total enzyme activity. Isolates with the highest total activity could be used for further studies such as purification of the enzyme and isolates characterization.http://www.sciencedirect.com/science/article/pii/S1978301916301681methylotrophic bacteriamethanol dehydrogenase |
| spellingShingle | DIANA ELIZABETH WATURANGI CATHERINE DELANY NICHOLAS CINDY OKTAVIA SUSANTO MAGGY THENAWIJAYA SUHARTONO Isolation and Identification of Methylotrophic Bacteria Producing Methanol Dehydrogenase from Human Feet and Mouth Hayati Journal of Biosciences methylotrophic bacteria methanol dehydrogenase |
| title | Isolation and Identification of Methylotrophic Bacteria Producing Methanol Dehydrogenase from Human Feet and Mouth |
| title_full | Isolation and Identification of Methylotrophic Bacteria Producing Methanol Dehydrogenase from Human Feet and Mouth |
| title_fullStr | Isolation and Identification of Methylotrophic Bacteria Producing Methanol Dehydrogenase from Human Feet and Mouth |
| title_full_unstemmed | Isolation and Identification of Methylotrophic Bacteria Producing Methanol Dehydrogenase from Human Feet and Mouth |
| title_short | Isolation and Identification of Methylotrophic Bacteria Producing Methanol Dehydrogenase from Human Feet and Mouth |
| title_sort | isolation and identification of methylotrophic bacteria producing methanol dehydrogenase from human feet and mouth |
| topic | methylotrophic bacteria methanol dehydrogenase |
| url | http://www.sciencedirect.com/science/article/pii/S1978301916301681 |
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