Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common Bean
Fusarium wilt (<i>Fusarium oxysporum</i> f. sp. <i>phaseoli</i>, <i>Fop</i>) is one of the main fungal soil diseases in common bean. The aim of the present study was to identify genomic regions associated with <i>Fop</i> resistance through genome-wide...
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MDPI AG
2021-05-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/5/765 |
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| author | Jean Fausto de Carvalho Paulino Caléo Panhoca de Almeida César Júnior Bueno Qijian Song Roberto Fritsche-Neto Sérgio Augusto Morais Carbonell Alisson Fernando Chiorato Luciana Lasry Benchimol-Reis |
| author_facet | Jean Fausto de Carvalho Paulino Caléo Panhoca de Almeida César Júnior Bueno Qijian Song Roberto Fritsche-Neto Sérgio Augusto Morais Carbonell Alisson Fernando Chiorato Luciana Lasry Benchimol-Reis |
| author_sort | Jean Fausto de Carvalho Paulino |
| collection | DOAJ |
| description | Fusarium wilt (<i>Fusarium oxysporum</i> f. sp. <i>phaseoli</i>, <i>Fop</i>) is one of the main fungal soil diseases in common bean. The aim of the present study was to identify genomic regions associated with <i>Fop</i> resistance through genome-wide association studies (GWAS) in a Mesoamerican Diversity Panel (MDP) and to identify potential common bean sources of <i>Fop</i>’s resistance. The MDP was genotyped with BARCBean6K_3BeadChip and evaluated for <i>Fop</i> resistance with two different monosporic strains using the root-dip method. Disease severity rating (DSR) and the area under the disease progress curve (AUDPC), at 21 days after inoculation (DAI), were used for GWAS performed with FarmCPU model. The <i>p</i>-value of each SNP was determined by resampling method and Bonferroni test. For UFV01 strain, two significant single nucleotide polymorphisms (SNPs) were mapped on the Pv05 and Pv11 for AUDPC, and the same SNP (ss715648096) on Pv11 was associated with AUDPC and DSR. Another SNP, mapped on Pv03, showed significance for DSR. Regarding IAC18001 strain, significant SNPs on Pv03, Pv04, Pv05, Pv07 and on Pv01, Pv05, and Pv10 were observed. Putative candidate genes related to nucleotide-binding sites and carboxy-terminal leucine-rich repeats were identified. The markers may be important future tools for genomic selection to <i>Fop</i> disease resistance in beans. |
| first_indexed | 2024-03-10T11:19:13Z |
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| id | doaj.art-8b3c60df231e4833a6c0fd2bf0d7328e |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T11:19:13Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
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| series | Genes |
| spelling | doaj.art-8b3c60df231e4833a6c0fd2bf0d7328e2023-11-21T20:12:13ZengMDPI AGGenes2073-44252021-05-0112576510.3390/genes12050765Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common BeanJean Fausto de Carvalho Paulino0Caléo Panhoca de Almeida1César Júnior Bueno2Qijian Song3Roberto Fritsche-Neto4Sérgio Augusto Morais Carbonell5Alisson Fernando Chiorato6Luciana Lasry Benchimol-Reis7Centro de Recursos Genéticos Vegetais, Instituto Agronômico, Campinas 13075-630, SP, BrazilCentro de Recursos Genéticos Vegetais, Instituto Agronômico, Campinas 13075-630, SP, BrazilCentro Avançado de Pesquisa em Proteção de Plantas e Saúde Animal, Instituto Biológico, Campinas 13101-680, SP, BrazilSoybean Genomics and Improvement Laboratory, US Department of Agriculture, Agricultural Research Service (USDA-ARS), Beltsville, MD 20705, USADepartment of Genetics, ‘Luiz de Queiroz’ Agriculture College, University of Sao Paulo, Piracicaba 13418-900, SP, BrazilCentro de Grãos e Fibras, Instituto Agronômico, Campinas 13075-630, SP, BrazilCentro de Grãos e Fibras, Instituto Agronômico, Campinas 13075-630, SP, BrazilCentro de Recursos Genéticos Vegetais, Instituto Agronômico, Campinas 13075-630, SP, BrazilFusarium wilt (<i>Fusarium oxysporum</i> f. sp. <i>phaseoli</i>, <i>Fop</i>) is one of the main fungal soil diseases in common bean. The aim of the present study was to identify genomic regions associated with <i>Fop</i> resistance through genome-wide association studies (GWAS) in a Mesoamerican Diversity Panel (MDP) and to identify potential common bean sources of <i>Fop</i>’s resistance. The MDP was genotyped with BARCBean6K_3BeadChip and evaluated for <i>Fop</i> resistance with two different monosporic strains using the root-dip method. Disease severity rating (DSR) and the area under the disease progress curve (AUDPC), at 21 days after inoculation (DAI), were used for GWAS performed with FarmCPU model. The <i>p</i>-value of each SNP was determined by resampling method and Bonferroni test. For UFV01 strain, two significant single nucleotide polymorphisms (SNPs) were mapped on the Pv05 and Pv11 for AUDPC, and the same SNP (ss715648096) on Pv11 was associated with AUDPC and DSR. Another SNP, mapped on Pv03, showed significance for DSR. Regarding IAC18001 strain, significant SNPs on Pv03, Pv04, Pv05, Pv07 and on Pv01, Pv05, and Pv10 were observed. Putative candidate genes related to nucleotide-binding sites and carboxy-terminal leucine-rich repeats were identified. The markers may be important future tools for genomic selection to <i>Fop</i> disease resistance in beans.https://www.mdpi.com/2073-4425/12/5/765<i>Phaseolus vulgaris</i> L.<i>Fusarium oxysporum</i> f. sp. <i>phaseoli</i>SNP markersdisease resistancemolecular breeding |
| spellingShingle | Jean Fausto de Carvalho Paulino Caléo Panhoca de Almeida César Júnior Bueno Qijian Song Roberto Fritsche-Neto Sérgio Augusto Morais Carbonell Alisson Fernando Chiorato Luciana Lasry Benchimol-Reis Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common Bean Genes <i>Phaseolus vulgaris</i> L. <i>Fusarium oxysporum</i> f. sp. <i>phaseoli</i> SNP markers disease resistance molecular breeding |
| title | Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common Bean |
| title_full | Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common Bean |
| title_fullStr | Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common Bean |
| title_full_unstemmed | Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common Bean |
| title_short | Genome-Wide Association Study Reveals Genomic Regions Associated with Fusarium Wilt Resistance in Common Bean |
| title_sort | genome wide association study reveals genomic regions associated with fusarium wilt resistance in common bean |
| topic | <i>Phaseolus vulgaris</i> L. <i>Fusarium oxysporum</i> f. sp. <i>phaseoli</i> SNP markers disease resistance molecular breeding |
| url | https://www.mdpi.com/2073-4425/12/5/765 |
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