Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs
Middle East respiratory syndrome coronavirus (MERS-CoV) infection can cause fatal pulmonary inflammatory disease in humans. Contrarily, camelids and bats are the main reservoir hosts, tolerant for MERS-CoV replication without suffering clinical disease. Here, we isolated cervical lymph node (LN) cel...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-06-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1205080/full |
| _version_ | 1797804602047856640 |
|---|---|
| author | Jordi Rodon Jordi Rodon Nigeer Te Nigeer Te Joaquim Segalés Joaquim Segalés Júlia Vergara-Alert Júlia Vergara-Alert Albert Bensaid Albert Bensaid |
| author_facet | Jordi Rodon Jordi Rodon Nigeer Te Nigeer Te Joaquim Segalés Joaquim Segalés Júlia Vergara-Alert Júlia Vergara-Alert Albert Bensaid Albert Bensaid |
| author_sort | Jordi Rodon |
| collection | DOAJ |
| description | Middle East respiratory syndrome coronavirus (MERS-CoV) infection can cause fatal pulmonary inflammatory disease in humans. Contrarily, camelids and bats are the main reservoir hosts, tolerant for MERS-CoV replication without suffering clinical disease. Here, we isolated cervical lymph node (LN) cells from MERS-CoV convalescent llamas and pulsed them with two different viral strains (clades B and C). Viral replication was not supported in LN, but a cellular immune response was mounted. Reminiscent Th1 responses (IFN-γ, IL-2, IL-12) were elicited upon MERS-CoV sensing, accompanied by a marked and transient peak of antiviral responses (type I IFNs, IFN-λ3, ISGs, PRRs and TFs). Importantly, expression of inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-8) or inflammasome components (NLRP3, CASP1, PYCARD) was dampened. The role of IFN-λ3 to counterbalance inflammatory processes and bridge innate and adaptive immune responses in camelid species is discussed. Our findings shed light into key mechanisms on how reservoir species control MERS-CoV in the absence of clinical disease. |
| first_indexed | 2024-03-13T05:39:40Z |
| format | Article |
| id | doaj.art-8b3dae8799454fefbc9db4149ce5382b |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-13T05:39:40Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-8b3dae8799454fefbc9db4149ce5382b2023-06-14T04:33:45ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-06-011410.3389/fimmu.2023.12050801205080Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirsJordi Rodon0Jordi Rodon1Nigeer Te2Nigeer Te3Joaquim Segalés4Joaquim Segalés5Júlia Vergara-Alert6Júlia Vergara-Alert7Albert Bensaid8Albert Bensaid9Unitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Catalonia, SpainIRTA, Programa de Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Catalonia, SpainUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Catalonia, SpainIRTA, Programa de Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Catalonia, SpainUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Catalonia, SpainDepartament de Sanitat i Anatomia Animals, Facultat de Veterinaria, Universitat Autònoma de Barcelona (UAB), Catalonia, SpainUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Catalonia, SpainIRTA, Programa de Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Catalonia, SpainUnitat Mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Catalonia, SpainIRTA, Programa de Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Catalonia, SpainMiddle East respiratory syndrome coronavirus (MERS-CoV) infection can cause fatal pulmonary inflammatory disease in humans. Contrarily, camelids and bats are the main reservoir hosts, tolerant for MERS-CoV replication without suffering clinical disease. Here, we isolated cervical lymph node (LN) cells from MERS-CoV convalescent llamas and pulsed them with two different viral strains (clades B and C). Viral replication was not supported in LN, but a cellular immune response was mounted. Reminiscent Th1 responses (IFN-γ, IL-2, IL-12) were elicited upon MERS-CoV sensing, accompanied by a marked and transient peak of antiviral responses (type I IFNs, IFN-λ3, ISGs, PRRs and TFs). Importantly, expression of inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-8) or inflammasome components (NLRP3, CASP1, PYCARD) was dampened. The role of IFN-λ3 to counterbalance inflammatory processes and bridge innate and adaptive immune responses in camelid species is discussed. Our findings shed light into key mechanisms on how reservoir species control MERS-CoV in the absence of clinical disease.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1205080/fullcamelidscytokinesimmune responsesllamalymph nodelymphocyte |
| spellingShingle | Jordi Rodon Jordi Rodon Nigeer Te Nigeer Te Joaquim Segalés Joaquim Segalés Júlia Vergara-Alert Júlia Vergara-Alert Albert Bensaid Albert Bensaid Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs Frontiers in Immunology camelids cytokines immune responses llama lymph node lymphocyte |
| title | Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs |
| title_full | Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs |
| title_fullStr | Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs |
| title_full_unstemmed | Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs |
| title_short | Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs |
| title_sort | enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon mers cov sensing bridging innate and adaptive cellular immune responses in camelid reservoirs |
| topic | camelids cytokines immune responses llama lymph node lymphocyte |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1205080/full |
| work_keys_str_mv | AT jordirodon enhancedantiviralimmunityanddampenedinflammationinllamalymphnodesuponmerscovsensingbridginginnateandadaptivecellularimmuneresponsesincamelidreservoirs AT jordirodon enhancedantiviralimmunityanddampenedinflammationinllamalymphnodesuponmerscovsensingbridginginnateandadaptivecellularimmuneresponsesincamelidreservoirs AT nigeerte enhancedantiviralimmunityanddampenedinflammationinllamalymphnodesuponmerscovsensingbridginginnateandadaptivecellularimmuneresponsesincamelidreservoirs AT nigeerte enhancedantiviralimmunityanddampenedinflammationinllamalymphnodesuponmerscovsensingbridginginnateandadaptivecellularimmuneresponsesincamelidreservoirs AT joaquimsegales enhancedantiviralimmunityanddampenedinflammationinllamalymphnodesuponmerscovsensingbridginginnateandadaptivecellularimmuneresponsesincamelidreservoirs AT joaquimsegales enhancedantiviralimmunityanddampenedinflammationinllamalymphnodesuponmerscovsensingbridginginnateandadaptivecellularimmuneresponsesincamelidreservoirs AT juliavergaraalert enhancedantiviralimmunityanddampenedinflammationinllamalymphnodesuponmerscovsensingbridginginnateandadaptivecellularimmuneresponsesincamelidreservoirs AT juliavergaraalert enhancedantiviralimmunityanddampenedinflammationinllamalymphnodesuponmerscovsensingbridginginnateandadaptivecellularimmuneresponsesincamelidreservoirs AT albertbensaid enhancedantiviralimmunityanddampenedinflammationinllamalymphnodesuponmerscovsensingbridginginnateandadaptivecellularimmuneresponsesincamelidreservoirs AT albertbensaid enhancedantiviralimmunityanddampenedinflammationinllamalymphnodesuponmerscovsensingbridginginnateandadaptivecellularimmuneresponsesincamelidreservoirs |