Antimicrobial activity of some seaweeds species from Red sea, against multidrug resistant bacteria
This study evaluates the antibacterial activity of diethyl ether, methanol, ethanol and chloroform extracts of red algae Ceramium rubrum (Rhodophyta), Sargassum vulgare, Sargassum fusiforme and Padina pavonia (Phaeophyta) collected from Red sea, Egypt. The algal extracts were tested for their antiba...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-03-01
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| Series: | Egyptian Journal of Aquatic Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687428515000849 |
| _version_ | 1819120756211056640 |
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| author | Shimaa M. El Shafay Samh S. Ali Mostafa M. El-Sheekh |
| author_facet | Shimaa M. El Shafay Samh S. Ali Mostafa M. El-Sheekh |
| author_sort | Shimaa M. El Shafay |
| collection | DOAJ |
| description | This study evaluates the antibacterial activity of diethyl ether, methanol, ethanol and chloroform extracts of red algae Ceramium rubrum (Rhodophyta), Sargassum vulgare, Sargassum fusiforme and Padina pavonia (Phaeophyta) collected from Red sea, Egypt. The algal extracts were tested for their antibacterial activity against ten multidrug resistant clinical isolates of Gram positive and Gram negative bacteria. The highest inhibition activity among all extracts was obtained with 100 μl diethyl ether extract S. fusiforme against Staphylococcus aureus 2 and 50 μl ethanol extract of S. vulgare against Klebsiella pneumoniae. The algal extract of S. fusiforme and S. vulgare was characterized by Gas chromatography–mass spectrometry (GC–MS). The compounds with antimicrobial activity were identified, such as phenols, terpenes, acetogenins, indoles, fatty acids and volatile halogenated hydrocarbons. Transmission electron microscopy was applied for determining the morphological changes in S. aureus 2 and K. pneumonia treated with 100 μl diethyl ether extract of S. fusiforme and 50 μl ethanol extract of S. vulgare, respectively. Perforation of cell wall, leakage of cytoplasmic contents, severe distortion of outer cell shape, inner chromatin mild scattered cytoplasmic vacuolation, rupture of cell wall, and decreased cell size for both bacterial isolates treated with 100 μl diethyl ether of S. fusiforme extract and 50 μl S. vulgare ethanolic extract were recorded. |
| first_indexed | 2024-12-22T06:25:43Z |
| format | Article |
| id | doaj.art-8b3f72547892447f81748e247431df44 |
| institution | Directory Open Access Journal |
| issn | 1687-4285 |
| language | English |
| last_indexed | 2024-12-22T06:25:43Z |
| publishDate | 2016-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Egyptian Journal of Aquatic Research |
| spelling | doaj.art-8b3f72547892447f81748e247431df442022-12-21T18:35:51ZengElsevierEgyptian Journal of Aquatic Research1687-42852016-03-01421657410.1016/j.ejar.2015.11.006Antimicrobial activity of some seaweeds species from Red sea, against multidrug resistant bacteriaShimaa M. El ShafaySamh S. AliMostafa M. El-SheekhThis study evaluates the antibacterial activity of diethyl ether, methanol, ethanol and chloroform extracts of red algae Ceramium rubrum (Rhodophyta), Sargassum vulgare, Sargassum fusiforme and Padina pavonia (Phaeophyta) collected from Red sea, Egypt. The algal extracts were tested for their antibacterial activity against ten multidrug resistant clinical isolates of Gram positive and Gram negative bacteria. The highest inhibition activity among all extracts was obtained with 100 μl diethyl ether extract S. fusiforme against Staphylococcus aureus 2 and 50 μl ethanol extract of S. vulgare against Klebsiella pneumoniae. The algal extract of S. fusiforme and S. vulgare was characterized by Gas chromatography–mass spectrometry (GC–MS). The compounds with antimicrobial activity were identified, such as phenols, terpenes, acetogenins, indoles, fatty acids and volatile halogenated hydrocarbons. Transmission electron microscopy was applied for determining the morphological changes in S. aureus 2 and K. pneumonia treated with 100 μl diethyl ether extract of S. fusiforme and 50 μl ethanol extract of S. vulgare, respectively. Perforation of cell wall, leakage of cytoplasmic contents, severe distortion of outer cell shape, inner chromatin mild scattered cytoplasmic vacuolation, rupture of cell wall, and decreased cell size for both bacterial isolates treated with 100 μl diethyl ether of S. fusiforme extract and 50 μl S. vulgare ethanolic extract were recorded.http://www.sciencedirect.com/science/article/pii/S1687428515000849Seaweed extractsAntimicrobial activityBacteriaBiochemical analyses |
| spellingShingle | Shimaa M. El Shafay Samh S. Ali Mostafa M. El-Sheekh Antimicrobial activity of some seaweeds species from Red sea, against multidrug resistant bacteria Egyptian Journal of Aquatic Research Seaweed extracts Antimicrobial activity Bacteria Biochemical analyses |
| title | Antimicrobial activity of some seaweeds species from Red sea, against multidrug resistant bacteria |
| title_full | Antimicrobial activity of some seaweeds species from Red sea, against multidrug resistant bacteria |
| title_fullStr | Antimicrobial activity of some seaweeds species from Red sea, against multidrug resistant bacteria |
| title_full_unstemmed | Antimicrobial activity of some seaweeds species from Red sea, against multidrug resistant bacteria |
| title_short | Antimicrobial activity of some seaweeds species from Red sea, against multidrug resistant bacteria |
| title_sort | antimicrobial activity of some seaweeds species from red sea against multidrug resistant bacteria |
| topic | Seaweed extracts Antimicrobial activity Bacteria Biochemical analyses |
| url | http://www.sciencedirect.com/science/article/pii/S1687428515000849 |
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