Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides
Abstract Background Increasing reports of high-resistant Triatominae populations concerns scientists and sanitarians as little is known about the factors behind the occurrence of such phenotype and its real impact on vector control strategies. Moreover, the utilization of a large variety of methodol...
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| Format: | Article |
| Language: | English |
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BMC
2015-02-01
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| Series: | Parasites & Vectors |
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| Online Access: | https://doi.org/10.1186/s13071-015-0726-4 |
| _version_ | 1797811904262963200 |
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| author | Grasielle Caldas D’Ávila Pessoa Letícia Cavalari Pinheiro Marcela Lencine Ferraz Bernardino Vaz de Mello Liléia Diotaiuti |
| author_facet | Grasielle Caldas D’Ávila Pessoa Letícia Cavalari Pinheiro Marcela Lencine Ferraz Bernardino Vaz de Mello Liléia Diotaiuti |
| author_sort | Grasielle Caldas D’Ávila Pessoa |
| collection | DOAJ |
| description | Abstract Background Increasing reports of high-resistant Triatominae populations concerns scientists and sanitarians as little is known about the factors behind the occurrence of such phenotype and its real impact on vector control strategies. Moreover, the utilization of a large variety of methodologies hinder the comparison of the reported studies. Methods This work aims to review laboratory bioassays, redefining the assessed biological features (age, generation and insecticide application area) and technical procedures (mortality recording time and the ideal diagnostic dose). Results Results were not influenced by the insecticide application area in nymphs or by their generation. Three days-old specimen’s revealed lower susceptibility to the tested insecticide. We determined that it is more appropriate to record mortality 72 h after treatment with insecticide, as well as using a diagnostic dose of 1xDL99. Conclusion This work suggests more adequate methodological parameters for assessing insecticide resistance in triatomines, which also allows the comparison of results obtained by different research groups. For laboratory bioassays, we recommend: 1) the use of first instar nymphs from first or second generation; 2) 3 day-old specimens; 2) application of insecticide in the dorsal or ventral abdomen area; 3) mortality recording 72 h after treatment with pyrethroids and 4) a diagnostic dose of 1x LD99. |
| first_indexed | 2024-03-13T07:29:33Z |
| format | Article |
| id | doaj.art-8b3f91ba81aa42648eb5dae3df014ea2 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:29:33Z |
| publishDate | 2015-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-8b3f91ba81aa42648eb5dae3df014ea22023-06-04T11:09:47ZengBMCParasites & Vectors1756-33052015-02-01811510.1186/s13071-015-0726-4Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticidesGrasielle Caldas D’Ávila Pessoa0Letícia Cavalari Pinheiro1Marcela Lencine Ferraz2Bernardino Vaz de Mello3Liléia Diotaiuti4Laboratório de Triatomíneos e Epidemiologia da Doença de Chagas, Centro de Pesquisa René RachouLaboratório de Triatomíneos e Epidemiologia da Doença de Chagas, Centro de Pesquisa René RachouSecretaria de Saúde do Estado de Minas GeraisSecretaria de Saúde do Estado de Minas GeraisLaboratório de Triatomíneos e Epidemiologia da Doença de Chagas, Centro de Pesquisa René RachouAbstract Background Increasing reports of high-resistant Triatominae populations concerns scientists and sanitarians as little is known about the factors behind the occurrence of such phenotype and its real impact on vector control strategies. Moreover, the utilization of a large variety of methodologies hinder the comparison of the reported studies. Methods This work aims to review laboratory bioassays, redefining the assessed biological features (age, generation and insecticide application area) and technical procedures (mortality recording time and the ideal diagnostic dose). Results Results were not influenced by the insecticide application area in nymphs or by their generation. Three days-old specimen’s revealed lower susceptibility to the tested insecticide. We determined that it is more appropriate to record mortality 72 h after treatment with insecticide, as well as using a diagnostic dose of 1xDL99. Conclusion This work suggests more adequate methodological parameters for assessing insecticide resistance in triatomines, which also allows the comparison of results obtained by different research groups. For laboratory bioassays, we recommend: 1) the use of first instar nymphs from first or second generation; 2) 3 day-old specimens; 2) application of insecticide in the dorsal or ventral abdomen area; 3) mortality recording 72 h after treatment with pyrethroids and 4) a diagnostic dose of 1x LD99.https://doi.org/10.1186/s13071-015-0726-4Insecticide resistanceBioassaysTriatominaeTriatoma sordida |
| spellingShingle | Grasielle Caldas D’Ávila Pessoa Letícia Cavalari Pinheiro Marcela Lencine Ferraz Bernardino Vaz de Mello Liléia Diotaiuti Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides Parasites & Vectors Insecticide resistance Bioassays Triatominae Triatoma sordida |
| title | Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides |
| title_full | Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides |
| title_fullStr | Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides |
| title_full_unstemmed | Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides |
| title_short | Standardization of laboratory bioassays for the study of Triatoma sordida susceptibility to pyrethroid insecticides |
| title_sort | standardization of laboratory bioassays for the study of triatoma sordida susceptibility to pyrethroid insecticides |
| topic | Insecticide resistance Bioassays Triatominae Triatoma sordida |
| url | https://doi.org/10.1186/s13071-015-0726-4 |
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