Leptin Receptor-Deficient db/db Mice Show Significant Heterogeneity in Response to High Non-heme Iron Diet
Recent studies have shown an association between iron homeostasis, obesity and diabetes. In this work, we investigated the differences in the metabolic status and inflammation in liver, pancreas and visceral adipose tissue of leptin receptor-deficient db/db mice dependent on high iron concentration...
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2021.741249/full |
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| author | Sabine Paeschke Karsten Winter Ingo Bechmann Nora Klöting Matthias Blüher Matthias Blüher Petra Baum Joanna Kosacka Joanna Kosacka Marcin Nowicki |
| author_facet | Sabine Paeschke Karsten Winter Ingo Bechmann Nora Klöting Matthias Blüher Matthias Blüher Petra Baum Joanna Kosacka Joanna Kosacka Marcin Nowicki |
| author_sort | Sabine Paeschke |
| collection | DOAJ |
| description | Recent studies have shown an association between iron homeostasis, obesity and diabetes. In this work, we investigated the differences in the metabolic status and inflammation in liver, pancreas and visceral adipose tissue of leptin receptor-deficient db/db mice dependent on high iron concentration diet. 3-month-old male BKS-Leprdb/db/JOrlRj (db/db) mice were divided into two groups, which were fed with different diets containing high iron (29 g/kg, n = 57) or standard iron (0.178 g/kg; n = 42) concentrations for 4 months. As anticipated, standard iron-fed db/db mice developed obesity and diabetes. However, high iron-fed mice exhibited a wide heterogeneity. By dividing into two subgroups at the diabetes level, non-diabetic subgroup 1 (<13.5 mmol/l, n = 30) significantly differed from diabetic subgroup two (>13.5 mmol/l, n = 27). Blood glucose concentration, HbA1c value, inflammation markers interleukin six and tumor necrosis factor α and heme oxygenase one in visceral adipose tissue were reduced in subgroup one compared to subgroup two. In contrast, body weight, C-peptide, serum insulin and serum iron concentrations, pancreatic islet and signal ratio as well as cholesterol, LDL and HDL levels were enhanced in subgroup one. While these significant differences require further studies and explanation, our results might also explain the often-contradictory results of the metabolic studies with db/db mice. |
| first_indexed | 2024-12-19T23:25:45Z |
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| id | doaj.art-8b3fa699b0144278ba70d62e21f8d4ab |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-19T23:25:45Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-8b3fa699b0144278ba70d62e21f8d4ab2022-12-21T20:01:51ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2021-09-01810.3389/fnut.2021.741249741249Leptin Receptor-Deficient db/db Mice Show Significant Heterogeneity in Response to High Non-heme Iron DietSabine Paeschke0Karsten Winter1Ingo Bechmann2Nora Klöting3Matthias Blüher4Matthias Blüher5Petra Baum6Joanna Kosacka7Joanna Kosacka8Marcin Nowicki9Institute of Anatomy, University of Leipzig, Leipzig, GermanyInstitute of Anatomy, University of Leipzig, Leipzig, GermanyInstitute of Anatomy, University of Leipzig, Leipzig, GermanyHelmholtz Institute for Metabolic, Obesity and Vascular Research (HI-MAG) of the Helmholtz Zentrum Munchen at the University of Leipzig, Leipzig, GermanyHelmholtz Institute for Metabolic, Obesity and Vascular Research (HI-MAG) of the Helmholtz Zentrum Munchen at the University of Leipzig, Leipzig, GermanyDepartment of Medicine, University of Leipzig, Leipzig, GermanyDepartment of Neurology, University of Leipzig, Leipzig, GermanyDepartment of Medicine, University of Leipzig, Leipzig, GermanyApplied Molecular Hepatology Lab, Department of Visceral, Transplant, Thoracic and Vascular Surgery, University of Leipzig Medical Center, Leipzig, GermanyInstitute of Anatomy, University of Leipzig, Leipzig, GermanyRecent studies have shown an association between iron homeostasis, obesity and diabetes. In this work, we investigated the differences in the metabolic status and inflammation in liver, pancreas and visceral adipose tissue of leptin receptor-deficient db/db mice dependent on high iron concentration diet. 3-month-old male BKS-Leprdb/db/JOrlRj (db/db) mice were divided into two groups, which were fed with different diets containing high iron (29 g/kg, n = 57) or standard iron (0.178 g/kg; n = 42) concentrations for 4 months. As anticipated, standard iron-fed db/db mice developed obesity and diabetes. However, high iron-fed mice exhibited a wide heterogeneity. By dividing into two subgroups at the diabetes level, non-diabetic subgroup 1 (<13.5 mmol/l, n = 30) significantly differed from diabetic subgroup two (>13.5 mmol/l, n = 27). Blood glucose concentration, HbA1c value, inflammation markers interleukin six and tumor necrosis factor α and heme oxygenase one in visceral adipose tissue were reduced in subgroup one compared to subgroup two. In contrast, body weight, C-peptide, serum insulin and serum iron concentrations, pancreatic islet and signal ratio as well as cholesterol, LDL and HDL levels were enhanced in subgroup one. While these significant differences require further studies and explanation, our results might also explain the often-contradictory results of the metabolic studies with db/db mice.https://www.frontiersin.org/articles/10.3389/fnut.2021.741249/fullironobesitydiabetes mellitusmetabolisminflammationdb/db mice |
| spellingShingle | Sabine Paeschke Karsten Winter Ingo Bechmann Nora Klöting Matthias Blüher Matthias Blüher Petra Baum Joanna Kosacka Joanna Kosacka Marcin Nowicki Leptin Receptor-Deficient db/db Mice Show Significant Heterogeneity in Response to High Non-heme Iron Diet Frontiers in Nutrition iron obesity diabetes mellitus metabolism inflammation db/db mice |
| title | Leptin Receptor-Deficient db/db Mice Show Significant Heterogeneity in Response to High Non-heme Iron Diet |
| title_full | Leptin Receptor-Deficient db/db Mice Show Significant Heterogeneity in Response to High Non-heme Iron Diet |
| title_fullStr | Leptin Receptor-Deficient db/db Mice Show Significant Heterogeneity in Response to High Non-heme Iron Diet |
| title_full_unstemmed | Leptin Receptor-Deficient db/db Mice Show Significant Heterogeneity in Response to High Non-heme Iron Diet |
| title_short | Leptin Receptor-Deficient db/db Mice Show Significant Heterogeneity in Response to High Non-heme Iron Diet |
| title_sort | leptin receptor deficient db db mice show significant heterogeneity in response to high non heme iron diet |
| topic | iron obesity diabetes mellitus metabolism inflammation db/db mice |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2021.741249/full |
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