Metabolic engineering of the L-phenylalanine pathway in <it>Escherichia coli </it>for the production of S- or R-mandelic acid
<p>Abstract</p> <p>Background</p> <p>Mandelic acid (MA), an important component in pharmaceutical syntheses, is currently produced exclusively via petrochemical processes. Growing concerns over the environment and fossil energy costs have inspired a quest to develop alt...
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BMC
2011-09-01
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| Series: | Microbial Cell Factories |
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| Online Access: | http://www.microbialcellfactories.com/content/10/1/71 |
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| author | Yang Chen Jiang Weihong Liu Yingmiao Sun Bingbing Liu Lixia Ning Yuanyuan Sun Zhoutong Yang Sheng |
| author_facet | Yang Chen Jiang Weihong Liu Yingmiao Sun Bingbing Liu Lixia Ning Yuanyuan Sun Zhoutong Yang Sheng |
| author_sort | Yang Chen |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Mandelic acid (MA), an important component in pharmaceutical syntheses, is currently produced exclusively via petrochemical processes. Growing concerns over the environment and fossil energy costs have inspired a quest to develop alternative routes to MA using renewable resources. Herein we report the first direct route to optically pure MA from glucose via genetic modification of the L-phenylalanine pathway in <it>E. coli</it>.</p> <p>Results</p> <p>The introduction of hydroxymandelate synthase (HmaS) from <it>Amycolatopsis orientalis </it>into <it>E. coli </it>led to a yield of 0.092 g/L S-MA. By combined deletion of competing pathways, further optimization of S-MA production was achieved, and the yield reached 0.74 g/L within 24 h. To produce R-MA, hydroxymandelate oxidase (Hmo) from <it>Streptomyces coelicolor </it>and D-mandelate dehydrogenase (DMD) from <it>Rhodotorula graminis </it>were co-expressed in an S-MA-producing strain, and the resulting strain was capable of producing 0.68 g/L R-MA. Finally, phenylpyruvate feeding experiments suggest that HmaS is a potential bottleneck to further improvement in yields.</p> <p>Conclusions</p> <p>We have constructed <it>E. coli </it>strains that successfully accomplished the production of S- and R-MA directly from glucose. Our work provides the first example of the completely fermentative production of S- and R-MA from renewable feedstock.</p> |
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| id | doaj.art-8b4044127b314ae3b5a8a50290d2c218 |
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| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-14T06:34:05Z |
| publishDate | 2011-09-01 |
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| series | Microbial Cell Factories |
| spelling | doaj.art-8b4044127b314ae3b5a8a50290d2c2182022-12-21T23:13:25ZengBMCMicrobial Cell Factories1475-28592011-09-011017110.1186/1475-2859-10-71Metabolic engineering of the L-phenylalanine pathway in <it>Escherichia coli </it>for the production of S- or R-mandelic acidYang ChenJiang WeihongLiu YingmiaoSun BingbingLiu LixiaNing YuanyuanSun ZhoutongYang Sheng<p>Abstract</p> <p>Background</p> <p>Mandelic acid (MA), an important component in pharmaceutical syntheses, is currently produced exclusively via petrochemical processes. Growing concerns over the environment and fossil energy costs have inspired a quest to develop alternative routes to MA using renewable resources. Herein we report the first direct route to optically pure MA from glucose via genetic modification of the L-phenylalanine pathway in <it>E. coli</it>.</p> <p>Results</p> <p>The introduction of hydroxymandelate synthase (HmaS) from <it>Amycolatopsis orientalis </it>into <it>E. coli </it>led to a yield of 0.092 g/L S-MA. By combined deletion of competing pathways, further optimization of S-MA production was achieved, and the yield reached 0.74 g/L within 24 h. To produce R-MA, hydroxymandelate oxidase (Hmo) from <it>Streptomyces coelicolor </it>and D-mandelate dehydrogenase (DMD) from <it>Rhodotorula graminis </it>were co-expressed in an S-MA-producing strain, and the resulting strain was capable of producing 0.68 g/L R-MA. Finally, phenylpyruvate feeding experiments suggest that HmaS is a potential bottleneck to further improvement in yields.</p> <p>Conclusions</p> <p>We have constructed <it>E. coli </it>strains that successfully accomplished the production of S- and R-MA directly from glucose. Our work provides the first example of the completely fermentative production of S- and R-MA from renewable feedstock.</p>http://www.microbialcellfactories.com/content/10/1/71<it>Escherichia coli</it>fermentationfine chemicalsmetabolic engineeringrenewable resourcesR-mandelic acidS-mandelic acid |
| spellingShingle | Yang Chen Jiang Weihong Liu Yingmiao Sun Bingbing Liu Lixia Ning Yuanyuan Sun Zhoutong Yang Sheng Metabolic engineering of the L-phenylalanine pathway in <it>Escherichia coli </it>for the production of S- or R-mandelic acid Microbial Cell Factories <it>Escherichia coli</it> fermentation fine chemicals metabolic engineering renewable resources R-mandelic acid S-mandelic acid |
| title | Metabolic engineering of the L-phenylalanine pathway in <it>Escherichia coli </it>for the production of S- or R-mandelic acid |
| title_full | Metabolic engineering of the L-phenylalanine pathway in <it>Escherichia coli </it>for the production of S- or R-mandelic acid |
| title_fullStr | Metabolic engineering of the L-phenylalanine pathway in <it>Escherichia coli </it>for the production of S- or R-mandelic acid |
| title_full_unstemmed | Metabolic engineering of the L-phenylalanine pathway in <it>Escherichia coli </it>for the production of S- or R-mandelic acid |
| title_short | Metabolic engineering of the L-phenylalanine pathway in <it>Escherichia coli </it>for the production of S- or R-mandelic acid |
| title_sort | metabolic engineering of the l phenylalanine pathway in it escherichia coli it for the production of s or r mandelic acid |
| topic | <it>Escherichia coli</it> fermentation fine chemicals metabolic engineering renewable resources R-mandelic acid S-mandelic acid |
| url | http://www.microbialcellfactories.com/content/10/1/71 |
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