RNA-seq data exploration after trypanosome RNA-binding protein UBP1 expression is altered by CRISPR-Cas9 gene editing and overexpression
Previous studies have shown that overexpression of the Trypanosoma cruzi U-rich RNA-binding protein 1 (TcUBP1) in insect-dwelling epimastigotes results in a gene expression pattern resembling that of the infective form of the pathogen. Here, we used CRISPR-Cas9-induced edition of TcUBP1 and full-len...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340924001276 |
| _version_ | 1797256355680092160 |
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| author | Karina B. Sabalette Vanina A. Campo José R. Sotelo-Silveira Pablo Smircich Javier G. De Gaudenzi |
| author_facet | Karina B. Sabalette Vanina A. Campo José R. Sotelo-Silveira Pablo Smircich Javier G. De Gaudenzi |
| author_sort | Karina B. Sabalette |
| collection | DOAJ |
| description | Previous studies have shown that overexpression of the Trypanosoma cruzi U-rich RNA-binding protein 1 (TcUBP1) in insect-dwelling epimastigotes results in a gene expression pattern resembling that of the infective form of the pathogen. Here, we used CRISPR-Cas9-induced edition of TcUBP1 and full-length protein overexpression in epimastigote cells to monitor transcriptomic changes during the epimastigote-to-metacyclic trypomastigote stage transition of T. cruzi. This dataset includes the bioinformatics analysis of three different RNA-seq samples, each with three biological replicates, showing differential mRNA abundances. The current transcriptome report has the potential to shed light on the quantitative variances in the expression of significantup- or down-regulated mRNAs as a consequence of the levels of the UBP1 protein. Raw data files were deposited at the NCBI Sequence Read Archive - SRA at http://ncbi.nlm.nih.gov/Traces/sra/sra.cgi with accession numbers PRJNA907231 and PRJNA949967. |
| first_indexed | 2024-03-08T00:00:55Z |
| format | Article |
| id | doaj.art-8b405d9128024c9a82da4e4a9040cae7 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-04-24T22:20:26Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-8b405d9128024c9a82da4e4a9040cae72024-03-20T06:09:55ZengElsevierData in Brief2352-34092024-04-0153110156RNA-seq data exploration after trypanosome RNA-binding protein UBP1 expression is altered by CRISPR-Cas9 gene editing and overexpressionKarina B. Sabalette0Vanina A. Campo1José R. Sotelo-Silveira2Pablo Smircich3Javier G. De Gaudenzi4Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín - Consejo Nacional de Investigaciones Científicas y Técnicas, 1650 General San Martín, Prov. de Buenos Aires, Argentina; Escuela de Bio y Nanotecnologías (EByN), Universidad Nacional de San Martín, 1650 General San Martín, Prov. de Buenos Aires, ArgentinaInstituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín - Consejo Nacional de Investigaciones Científicas y Técnicas, 1650 General San Martín, Prov. de Buenos Aires, Argentina; Escuela de Bio y Nanotecnologías (EByN), Universidad Nacional de San Martín, 1650 General San Martín, Prov. de Buenos Aires, ArgentinaInstituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, Montevideo, CP 11600, Uruguay; Instituto de Biología, School of Sciences, Universidad de la República, Montevideo, UruguayInstituto de Investigaciones Biológicas Clemente Estable, Av. Italia 3318, Montevideo, CP 11600, Uruguay; Instituto de Biología, School of Sciences, Universidad de la República, Montevideo, UruguayInstituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín - Consejo Nacional de Investigaciones Científicas y Técnicas, 1650 General San Martín, Prov. de Buenos Aires, Argentina; Escuela de Bio y Nanotecnologías (EByN), Universidad Nacional de San Martín, 1650 General San Martín, Prov. de Buenos Aires, Argentina; Corresponding author at: Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín - Consejo Nacional de Investigaciones Científicas y Técnicas, 1650 General San Martín, Prov. de Buenos Aires, Argentina.Previous studies have shown that overexpression of the Trypanosoma cruzi U-rich RNA-binding protein 1 (TcUBP1) in insect-dwelling epimastigotes results in a gene expression pattern resembling that of the infective form of the pathogen. Here, we used CRISPR-Cas9-induced edition of TcUBP1 and full-length protein overexpression in epimastigote cells to monitor transcriptomic changes during the epimastigote-to-metacyclic trypomastigote stage transition of T. cruzi. This dataset includes the bioinformatics analysis of three different RNA-seq samples, each with three biological replicates, showing differential mRNA abundances. The current transcriptome report has the potential to shed light on the quantitative variances in the expression of significantup- or down-regulated mRNAs as a consequence of the levels of the UBP1 protein. Raw data files were deposited at the NCBI Sequence Read Archive - SRA at http://ncbi.nlm.nih.gov/Traces/sra/sra.cgi with accession numbers PRJNA907231 and PRJNA949967.http://www.sciencedirect.com/science/article/pii/S2352340924001276RNA-binding proteinTranscriptomicsTrypanosoma cruziDifferential gene expressionParasite differentiation |
| spellingShingle | Karina B. Sabalette Vanina A. Campo José R. Sotelo-Silveira Pablo Smircich Javier G. De Gaudenzi RNA-seq data exploration after trypanosome RNA-binding protein UBP1 expression is altered by CRISPR-Cas9 gene editing and overexpression Data in Brief RNA-binding protein Transcriptomics Trypanosoma cruzi Differential gene expression Parasite differentiation |
| title | RNA-seq data exploration after trypanosome RNA-binding protein UBP1 expression is altered by CRISPR-Cas9 gene editing and overexpression |
| title_full | RNA-seq data exploration after trypanosome RNA-binding protein UBP1 expression is altered by CRISPR-Cas9 gene editing and overexpression |
| title_fullStr | RNA-seq data exploration after trypanosome RNA-binding protein UBP1 expression is altered by CRISPR-Cas9 gene editing and overexpression |
| title_full_unstemmed | RNA-seq data exploration after trypanosome RNA-binding protein UBP1 expression is altered by CRISPR-Cas9 gene editing and overexpression |
| title_short | RNA-seq data exploration after trypanosome RNA-binding protein UBP1 expression is altered by CRISPR-Cas9 gene editing and overexpression |
| title_sort | rna seq data exploration after trypanosome rna binding protein ubp1 expression is altered by crispr cas9 gene editing and overexpression |
| topic | RNA-binding protein Transcriptomics Trypanosoma cruzi Differential gene expression Parasite differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S2352340924001276 |
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