| Summary: | Anthocyanin is a characteristic nutrient of purple cabbage, and phenylalanine ammonia-lyase (PAL) is the rate-limiting enzyme for the synthesis of anthocyanin by the phenylpropane pathway, which is an important part of plant secondary metabolism. In this research, 7 <i>BrPAL</i>, 8 <i>BoPAL</i>, and 15 <i>BnPAL</i> genes from genomes of <i>Brassica rapa</i>, <i>Brassica oleracea</i>, and <i>Brassica napus</i>, divided into four subgroups, evolved from 4 <i>PAL</i> genes in <i>Arabidopsis</i>. The amplification and evolution of the <i>BrPAL</i> gene are due to segmental duplication and purifying selection. <i>BrPAL</i> genes clustered in the same clade have similar intron/exon structures and motifs. The cis-regulatory elements are divided into four categories: light, growth and development, stress and hormones. The qRT-PCR assays showed that most <i>BrPAL</i> genes were upregulated by UVA, low temperature and MeJA and downregulated by FR, high temperature, salt, PEG, IAA, ABA and GA, and there was a positive correlation between anthocyanin content and gene expression. This study can be used as a source for the function of the cabbage <i>PAL</i> gene and its molecular mechanism of regulating anthocyanin synthesis and provides a theoretical basis for the molecular breeding of cabbage.
|
|---|