Size-selective downstream processing of virus particles and non-enveloped virus-like particles
Non-enveloped virus-like particles (VLPs) are versatile protein nanoparticles with great potential for biopharmaceutical applications. However, conventional protein downstream processing (DSP) and platform processes are often not easily applicable due to the large size of VLPs and virus particles (V...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1192050/full |
| _version_ | 1797820523871207424 |
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| author | Nils Hillebrandt Jürgen Hubbuch |
| author_facet | Nils Hillebrandt Jürgen Hubbuch |
| author_sort | Nils Hillebrandt |
| collection | DOAJ |
| description | Non-enveloped virus-like particles (VLPs) are versatile protein nanoparticles with great potential for biopharmaceutical applications. However, conventional protein downstream processing (DSP) and platform processes are often not easily applicable due to the large size of VLPs and virus particles (VPs) in general. The application of size-selective separation techniques offers to exploit the size difference between VPs and common host-cell impurities. Moreover, size-selective separation techniques offer the potential for wide applicability across different VPs. In this work, basic principles and applications of size-selective separation techniques are reviewed to highlight their potential in DSP of VPs. Finally, specific DSP steps for non-enveloped VLPs and their subunits are reviewed as well as the potential applications and benefits of size-selective separation techniques are shown. |
| first_indexed | 2024-03-13T09:39:39Z |
| format | Article |
| id | doaj.art-8b461955ba694c14aecfc6d39e17d03f |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-13T09:39:39Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-8b461955ba694c14aecfc6d39e17d03f2023-05-25T04:47:27ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-05-011110.3389/fbioe.2023.11920501192050Size-selective downstream processing of virus particles and non-enveloped virus-like particlesNils HillebrandtJürgen HubbuchNon-enveloped virus-like particles (VLPs) are versatile protein nanoparticles with great potential for biopharmaceutical applications. However, conventional protein downstream processing (DSP) and platform processes are often not easily applicable due to the large size of VLPs and virus particles (VPs) in general. The application of size-selective separation techniques offers to exploit the size difference between VPs and common host-cell impurities. Moreover, size-selective separation techniques offer the potential for wide applicability across different VPs. In this work, basic principles and applications of size-selective separation techniques are reviewed to highlight their potential in DSP of VPs. Finally, specific DSP steps for non-enveloped VLPs and their subunits are reviewed as well as the potential applications and benefits of size-selective separation techniques are shown.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1192050/fullvirus particlesvirus-like particlesvaccinesdownstream processingpurificationsize-selective |
| spellingShingle | Nils Hillebrandt Jürgen Hubbuch Size-selective downstream processing of virus particles and non-enveloped virus-like particles Frontiers in Bioengineering and Biotechnology virus particles virus-like particles vaccines downstream processing purification size-selective |
| title | Size-selective downstream processing of virus particles and non-enveloped virus-like particles |
| title_full | Size-selective downstream processing of virus particles and non-enveloped virus-like particles |
| title_fullStr | Size-selective downstream processing of virus particles and non-enveloped virus-like particles |
| title_full_unstemmed | Size-selective downstream processing of virus particles and non-enveloped virus-like particles |
| title_short | Size-selective downstream processing of virus particles and non-enveloped virus-like particles |
| title_sort | size selective downstream processing of virus particles and non enveloped virus like particles |
| topic | virus particles virus-like particles vaccines downstream processing purification size-selective |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1192050/full |
| work_keys_str_mv | AT nilshillebrandt sizeselectivedownstreamprocessingofvirusparticlesandnonenvelopedviruslikeparticles AT jurgenhubbuch sizeselectivedownstreamprocessingofvirusparticlesandnonenvelopedviruslikeparticles |