Maximizing Polysaccharides and Phycoerythrin in <i>Porphyridium purpureum</i> via the Addition of Exogenous Compounds: A Response-Surface-Methodology Approach
Phycoerythrin and polysaccharides have significant commercial value in medicine, cosmetics, and food industries due to their excellent bioactive functions. To maximize the production of biomass, phycoerythrin, and polysaccharides in <i>Porphyridium purpureum</i>, culture media were suppl...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/22/3/138 |
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| author | Sanjiong Yi Ai-Hua Zhang Jianke Huang Ting Yao Bo Feng Xinghu Zhou Yadong Hu Mingxuan Pan |
| author_facet | Sanjiong Yi Ai-Hua Zhang Jianke Huang Ting Yao Bo Feng Xinghu Zhou Yadong Hu Mingxuan Pan |
| author_sort | Sanjiong Yi |
| collection | DOAJ |
| description | Phycoerythrin and polysaccharides have significant commercial value in medicine, cosmetics, and food industries due to their excellent bioactive functions. To maximize the production of biomass, phycoerythrin, and polysaccharides in <i>Porphyridium purpureum</i>, culture media were supplemented with calcium gluconate (CG), magnesium gluconate (MG) and polypeptides (BT), and their optimal amounts were determined using the response surface methodology (RSM) based on three single-factor experiments. The optimal concentrations of CG, MG, and BT were determined to be 4, 12, and 2 g L<sup>−1</sup>, respectively. The RSM-based models indicated that biomass and phycoerythrin production were significantly affected only by MG and BT, respectively. However, polysaccharide production was significantly affected by the interactions between CG and BT and those between MG and BT, with no significant effect from BT alone. Using the optimized culture conditions, the maximum biomass (5.97 g L<sup>−1</sup>), phycoerythrin (102.95 mg L<sup>−1</sup>), and polysaccharide (1.42 g L<sup>−1</sup>) concentrations met and even surpassed the model-predicted maximums. After optimization, biomass, phycoerythrin, and polysaccharides concentrations increased by 132.3%, 27.97%, and 136.67%, respectively, compared to the control. Overall, this study establishes a strong foundation for the highly efficient production of phycoerythrin and polysaccharides using <i>P. purpureum</i>. |
| first_indexed | 2024-04-24T18:04:00Z |
| format | Article |
| id | doaj.art-8b46e8c061b149d98b9ed81facf28dc5 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-24T18:04:00Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-8b46e8c061b149d98b9ed81facf28dc52024-03-27T13:52:14ZengMDPI AGMarine Drugs1660-33972024-03-0122313810.3390/md22030138Maximizing Polysaccharides and Phycoerythrin in <i>Porphyridium purpureum</i> via the Addition of Exogenous Compounds: A Response-Surface-Methodology ApproachSanjiong Yi0Ai-Hua Zhang1Jianke Huang2Ting Yao3Bo Feng4Xinghu Zhou5Yadong Hu6Mingxuan Pan7Jiangsu Province Engineering Research Center for Marine Bio-Resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing 210098, ChinaJiangsu Province Engineering Research Center for Marine Bio-Resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing 210098, ChinaJiangsu Province Engineering Research Center for Marine Bio-Resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing 210098, ChinaJiangsu Province Engineering Research Center for Marine Bio-Resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing 210098, ChinaJiangsu Province Engineering Research Center for Marine Bio-Resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing 210098, ChinaJiangsu Innovation Center of Marine Bioresource, Jiangsu Coast Development Investment Co., Ltd., Jiangsu Coast Development Group Co., Ltd., Nanjing 210019, ChinaJiangsu Innovation Center of Marine Bioresource, Jiangsu Coast Development Investment Co., Ltd., Jiangsu Coast Development Group Co., Ltd., Nanjing 210019, ChinaJiangsu Innovation Center of Marine Bioresource, Jiangsu Coast Development Investment Co., Ltd., Jiangsu Coast Development Group Co., Ltd., Nanjing 210019, ChinaPhycoerythrin and polysaccharides have significant commercial value in medicine, cosmetics, and food industries due to their excellent bioactive functions. To maximize the production of biomass, phycoerythrin, and polysaccharides in <i>Porphyridium purpureum</i>, culture media were supplemented with calcium gluconate (CG), magnesium gluconate (MG) and polypeptides (BT), and their optimal amounts were determined using the response surface methodology (RSM) based on three single-factor experiments. The optimal concentrations of CG, MG, and BT were determined to be 4, 12, and 2 g L<sup>−1</sup>, respectively. The RSM-based models indicated that biomass and phycoerythrin production were significantly affected only by MG and BT, respectively. However, polysaccharide production was significantly affected by the interactions between CG and BT and those between MG and BT, with no significant effect from BT alone. Using the optimized culture conditions, the maximum biomass (5.97 g L<sup>−1</sup>), phycoerythrin (102.95 mg L<sup>−1</sup>), and polysaccharide (1.42 g L<sup>−1</sup>) concentrations met and even surpassed the model-predicted maximums. After optimization, biomass, phycoerythrin, and polysaccharides concentrations increased by 132.3%, 27.97%, and 136.67%, respectively, compared to the control. Overall, this study establishes a strong foundation for the highly efficient production of phycoerythrin and polysaccharides using <i>P. purpureum</i>.https://www.mdpi.com/1660-3397/22/3/138<i>Porphyridium purpureum</i>polysaccharidephycoerythrinresponse surface methodologypolypeptidesgluconate |
| spellingShingle | Sanjiong Yi Ai-Hua Zhang Jianke Huang Ting Yao Bo Feng Xinghu Zhou Yadong Hu Mingxuan Pan Maximizing Polysaccharides and Phycoerythrin in <i>Porphyridium purpureum</i> via the Addition of Exogenous Compounds: A Response-Surface-Methodology Approach Marine Drugs <i>Porphyridium purpureum</i> polysaccharide phycoerythrin response surface methodology polypeptides gluconate |
| title | Maximizing Polysaccharides and Phycoerythrin in <i>Porphyridium purpureum</i> via the Addition of Exogenous Compounds: A Response-Surface-Methodology Approach |
| title_full | Maximizing Polysaccharides and Phycoerythrin in <i>Porphyridium purpureum</i> via the Addition of Exogenous Compounds: A Response-Surface-Methodology Approach |
| title_fullStr | Maximizing Polysaccharides and Phycoerythrin in <i>Porphyridium purpureum</i> via the Addition of Exogenous Compounds: A Response-Surface-Methodology Approach |
| title_full_unstemmed | Maximizing Polysaccharides and Phycoerythrin in <i>Porphyridium purpureum</i> via the Addition of Exogenous Compounds: A Response-Surface-Methodology Approach |
| title_short | Maximizing Polysaccharides and Phycoerythrin in <i>Porphyridium purpureum</i> via the Addition of Exogenous Compounds: A Response-Surface-Methodology Approach |
| title_sort | maximizing polysaccharides and phycoerythrin in i porphyridium purpureum i via the addition of exogenous compounds a response surface methodology approach |
| topic | <i>Porphyridium purpureum</i> polysaccharide phycoerythrin response surface methodology polypeptides gluconate |
| url | https://www.mdpi.com/1660-3397/22/3/138 |
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