Sequential Continuous Mixotrophic and Phototrophic Cultivation Might Be a Cost-Effective Strategy for Astaxanthin Production From the Microalga Haematococcus lacustris
Although Haematococcus lacustris has been developed for astaxanthin production for decades, the production cost is still high. In order to modify the production processes, we proposed a novel strategy of cultivation, featured by sequential indoor continuous mixotrophic cultivation for the production...
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| Format: | Article |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.740533/full |
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| author | Mahammed Ilyas Khazi Liangtao Shi Fakhra Liaqat Yuxin Yang Xin Li Duanpeng Yang Jian Li |
| author_facet | Mahammed Ilyas Khazi Liangtao Shi Fakhra Liaqat Yuxin Yang Xin Li Duanpeng Yang Jian Li |
| author_sort | Mahammed Ilyas Khazi |
| collection | DOAJ |
| description | Although Haematococcus lacustris has been developed for astaxanthin production for decades, the production cost is still high. In order to modify the production processes, we proposed a novel strategy of cultivation, featured by sequential indoor continuous mixotrophic cultivation for the production of green cells followed by outdoor phototrophic induction for astaxanthin accumulation. The continuous mixotrophic cultivation was first optimized indoor, and then the seed culture of mixotrophic cultivation was inoculated into outdoor open raceway ponds for photoinduction. The results showed that mixotrophically grown cultures could efficiently grow without losing their photosynthetic efficiency and yielded higher biomass concentration (0.655 g L−1) and astaxanthin content (2.2% DW), compared to phototrophically grown seed culture controls. This novel strategy might be a promising alternative to the current approaches to advance the production technology of astaxanthin from microalgae. |
| first_indexed | 2024-12-19T00:28:11Z |
| format | Article |
| id | doaj.art-8b476861fd084ce9a0d6597359257aaf |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-19T00:28:11Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-8b476861fd084ce9a0d6597359257aaf2022-12-21T20:45:11ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-10-01910.3389/fbioe.2021.740533740533Sequential Continuous Mixotrophic and Phototrophic Cultivation Might Be a Cost-Effective Strategy for Astaxanthin Production From the Microalga Haematococcus lacustrisMahammed Ilyas Khazi0Liangtao Shi1Fakhra Liaqat2Yuxin Yang3Xin Li4Duanpeng Yang5Jian Li6Department of Research and Development, Panzhihua Gesala Biotechnology Inc., Panzhihua, ChinaInstitute of Tropical Eco-agriculture, Yunnan Academy of Agricultural Science, Kunming, ChinaDepartment of Research and Development, Panzhihua Gesala Biotechnology Inc., Panzhihua, ChinaSchool of Biological and Chemical Engineering, Panzhihua University, Panzhihua, ChinaSchool of Biological and Chemical Engineering, Panzhihua University, Panzhihua, ChinaSchool of Biological and Chemical Engineering, Panzhihua University, Panzhihua, ChinaSchool of Biological and Chemical Engineering, Panzhihua University, Panzhihua, ChinaAlthough Haematococcus lacustris has been developed for astaxanthin production for decades, the production cost is still high. In order to modify the production processes, we proposed a novel strategy of cultivation, featured by sequential indoor continuous mixotrophic cultivation for the production of green cells followed by outdoor phototrophic induction for astaxanthin accumulation. The continuous mixotrophic cultivation was first optimized indoor, and then the seed culture of mixotrophic cultivation was inoculated into outdoor open raceway ponds for photoinduction. The results showed that mixotrophically grown cultures could efficiently grow without losing their photosynthetic efficiency and yielded higher biomass concentration (0.655 g L−1) and astaxanthin content (2.2% DW), compared to phototrophically grown seed culture controls. This novel strategy might be a promising alternative to the current approaches to advance the production technology of astaxanthin from microalgae.https://www.frontiersin.org/articles/10.3389/fbioe.2021.740533/fullastaxanthinHaematococcusmixotrophymicroalgaecontinuous cultivation |
| spellingShingle | Mahammed Ilyas Khazi Liangtao Shi Fakhra Liaqat Yuxin Yang Xin Li Duanpeng Yang Jian Li Sequential Continuous Mixotrophic and Phototrophic Cultivation Might Be a Cost-Effective Strategy for Astaxanthin Production From the Microalga Haematococcus lacustris Frontiers in Bioengineering and Biotechnology astaxanthin Haematococcus mixotrophy microalgae continuous cultivation |
| title | Sequential Continuous Mixotrophic and Phototrophic Cultivation Might Be a Cost-Effective Strategy for Astaxanthin Production From the Microalga Haematococcus lacustris |
| title_full | Sequential Continuous Mixotrophic and Phototrophic Cultivation Might Be a Cost-Effective Strategy for Astaxanthin Production From the Microalga Haematococcus lacustris |
| title_fullStr | Sequential Continuous Mixotrophic and Phototrophic Cultivation Might Be a Cost-Effective Strategy for Astaxanthin Production From the Microalga Haematococcus lacustris |
| title_full_unstemmed | Sequential Continuous Mixotrophic and Phototrophic Cultivation Might Be a Cost-Effective Strategy for Astaxanthin Production From the Microalga Haematococcus lacustris |
| title_short | Sequential Continuous Mixotrophic and Phototrophic Cultivation Might Be a Cost-Effective Strategy for Astaxanthin Production From the Microalga Haematococcus lacustris |
| title_sort | sequential continuous mixotrophic and phototrophic cultivation might be a cost effective strategy for astaxanthin production from the microalga haematococcus lacustris |
| topic | astaxanthin Haematococcus mixotrophy microalgae continuous cultivation |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.740533/full |
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