Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid.
The ovarian, or coelomic, fluid that is released with the egg mass of many fishes is increasingly found to play an important role in several biological processes crucial for reproductive success. These include maintenance of oocyte fertility and developmental competence, prolonging of sperm motility...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25089903/?tool=EBI |
| _version_ | 1819181122612887552 |
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| author | Sheri L Johnson Marsha Villarroel Patrice Rosengrave Alan Carne Torsten Kleffmann P Mark Lokman Neil J Gemmell |
| author_facet | Sheri L Johnson Marsha Villarroel Patrice Rosengrave Alan Carne Torsten Kleffmann P Mark Lokman Neil J Gemmell |
| author_sort | Sheri L Johnson |
| collection | DOAJ |
| description | The ovarian, or coelomic, fluid that is released with the egg mass of many fishes is increasingly found to play an important role in several biological processes crucial for reproductive success. These include maintenance of oocyte fertility and developmental competence, prolonging of sperm motility, and enhancing sperm swimming speed. Here we examined if and how the proteome of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid varied among females and then sought to examine the composition of this fluid. Ovarian fluid in chinook salmon was analyzed using 1D SDS PAGE and LC-MS/MS tryptic digest screened against Mascot and Sequest databases. We found marked differences in the number and concentrations of proteins in salmon ovarian fluid across different females. A total of 174 proteins were identified in ovarian fluid, 47 of which were represented by six or more peptides, belonging to one of six Gene Ontology pathways. The response to chemical stimulus and response to hypoxia pathways were best represented, accounting for 26 of the 174 proteins. The current data set provides a resource that furthers our understanding of those factors that influence successful egg production and fertilisation in salmonids and other species. |
| first_indexed | 2024-12-22T22:25:13Z |
| format | Article |
| id | doaj.art-8b484866d6b44eaf8561eb98249b75b7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T22:25:13Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8b484866d6b44eaf8561eb98249b75b72022-12-21T18:10:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10415510.1371/journal.pone.0104155Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid.Sheri L JohnsonMarsha VillarroelPatrice RosengraveAlan CarneTorsten KleffmannP Mark LokmanNeil J GemmellThe ovarian, or coelomic, fluid that is released with the egg mass of many fishes is increasingly found to play an important role in several biological processes crucial for reproductive success. These include maintenance of oocyte fertility and developmental competence, prolonging of sperm motility, and enhancing sperm swimming speed. Here we examined if and how the proteome of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid varied among females and then sought to examine the composition of this fluid. Ovarian fluid in chinook salmon was analyzed using 1D SDS PAGE and LC-MS/MS tryptic digest screened against Mascot and Sequest databases. We found marked differences in the number and concentrations of proteins in salmon ovarian fluid across different females. A total of 174 proteins were identified in ovarian fluid, 47 of which were represented by six or more peptides, belonging to one of six Gene Ontology pathways. The response to chemical stimulus and response to hypoxia pathways were best represented, accounting for 26 of the 174 proteins. The current data set provides a resource that furthers our understanding of those factors that influence successful egg production and fertilisation in salmonids and other species.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25089903/?tool=EBI |
| spellingShingle | Sheri L Johnson Marsha Villarroel Patrice Rosengrave Alan Carne Torsten Kleffmann P Mark Lokman Neil J Gemmell Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid. PLoS ONE |
| title | Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid. |
| title_full | Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid. |
| title_fullStr | Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid. |
| title_full_unstemmed | Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid. |
| title_short | Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid. |
| title_sort | proteomic analysis of chinook salmon oncorhynchus tshawytscha ovarian fluid |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25089903/?tool=EBI |
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