Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants
The tuber necrotic strain of Potato virus Y (PVYNTN) causes widespread disease and has severe negative effects on the growth and yields of plants, especially those of the Solanaceae family. The consequences of residual toxicity and non-biodegradation of synthetic chemicals and pollution of the en...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Polish Academy of Sciences
2018-12-01
|
Series: | Journal of Plant Protection Research |
Subjects: | |
Online Access: | https://doi.org/10.24425/jppr.2018.124648 |
_version_ | 1819002482565578752 |
---|---|
author | Simin Emamzadeh Yazdi Julia Mulabisana Gerhard Prinsloo Michele Cloete Quenton Kritzinger |
author_facet | Simin Emamzadeh Yazdi Julia Mulabisana Gerhard Prinsloo Michele Cloete Quenton Kritzinger |
author_sort | Simin Emamzadeh Yazdi |
collection | DOAJ |
description | The tuber necrotic strain of Potato virus Y (PVYNTN) causes widespread disease and has
severe negative effects on the growth and yields of plants, especially those of the Solanaceae
family. The consequences of residual toxicity and non-biodegradation of synthetic chemicals
and pollution of the environment has led to investigations into new non-toxic and
biological treatments to control plant viral diseases. Ethanolic extracts of Bowiea volubilis
(bulbs), Cotyledon orbiculata (leaves), Gomphocarpus fruticosus (leaves), Merwilla plumbea
(dry and fresh bulbs), Nerium oleander (leaves), and the fruits and leaves of Strophanthus
speciosus, were evaluated against PVYNTN in vivo and in vitro. At a concentration of
20 mg · ml−1, ethanolic extracts of Strophanthus speciosus (leaves) and fruits (50 mg · ml−1)
significantly reduced the expression of PVYNTN symptoms on tobacco plants in vitro without
affecting the normal growth and development of the plant. Similarly, at 50 mg · ml−1,
N. oleander, C. orbiculata and B. volubilis (fresh bulbs) and S. speciousus leaves at
20 mg · ml−1 extracts showed significant differences in PVYNTN symptoms in the in vivo
experiment. Strophanthus speciosus leaf and fruit extracts showed significant inhibition in
the in vitro and in vivo assays and demonstrated that S. speciosus has potential to be used as
an antiphytoviral treatment. |
first_indexed | 2024-12-20T23:05:49Z |
format | Article |
id | doaj.art-8b4dbb11ef6d418b9532fc6c3dcecdc6 |
institution | Directory Open Access Journal |
issn | 1899-007X 1899-007X |
language | English |
last_indexed | 2024-12-20T23:05:49Z |
publishDate | 2018-12-01 |
publisher | Polish Academy of Sciences |
record_format | Article |
series | Journal of Plant Protection Research |
spelling | doaj.art-8b4dbb11ef6d418b9532fc6c3dcecdc62022-12-21T19:23:52ZengPolish Academy of SciencesJournal of Plant Protection Research1899-007X1899-007X2018-12-0158439540310.24425/jppr.2018.124648Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plantsSimin Emamzadeh Yazdi0Julia Mulabisana1Gerhard Prinsloo2Michele Cloete3Quenton Kritzinger4Department of Plant and Soil Sciences, University of Pretoria, Pretoria, South AfricaAgricultural Research Council, Vegetable and Ornamental Plants, Roodeplaat, Pretoria, South AfricaDepartment of Agriculture and Animal Health, University of South Africa (UNISA), Florida Campus, South AfricaAgricultural Research Council, Vegetable and Ornamental Plants, Roodeplaat, Pretoria, South AfricaDepartment of Plant and Soil Sciences, University of Pretoria, Pretoria, South AfricaThe tuber necrotic strain of Potato virus Y (PVYNTN) causes widespread disease and has severe negative effects on the growth and yields of plants, especially those of the Solanaceae family. The consequences of residual toxicity and non-biodegradation of synthetic chemicals and pollution of the environment has led to investigations into new non-toxic and biological treatments to control plant viral diseases. Ethanolic extracts of Bowiea volubilis (bulbs), Cotyledon orbiculata (leaves), Gomphocarpus fruticosus (leaves), Merwilla plumbea (dry and fresh bulbs), Nerium oleander (leaves), and the fruits and leaves of Strophanthus speciosus, were evaluated against PVYNTN in vivo and in vitro. At a concentration of 20 mg · ml−1, ethanolic extracts of Strophanthus speciosus (leaves) and fruits (50 mg · ml−1) significantly reduced the expression of PVYNTN symptoms on tobacco plants in vitro without affecting the normal growth and development of the plant. Similarly, at 50 mg · ml−1, N. oleander, C. orbiculata and B. volubilis (fresh bulbs) and S. speciousus leaves at 20 mg · ml−1 extracts showed significant differences in PVYNTN symptoms in the in vivo experiment. Strophanthus speciosus leaf and fruit extracts showed significant inhibition in the in vitro and in vivo assays and demonstrated that S. speciosus has potential to be used as an antiphytoviral treatment.https://doi.org/10.24425/jppr.2018.124648antiphytoviralcardiac glycosideDAS-ELISAplant extractsPotato virus Y (PVYNTN) |
spellingShingle | Simin Emamzadeh Yazdi Julia Mulabisana Gerhard Prinsloo Michele Cloete Quenton Kritzinger Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants Journal of Plant Protection Research antiphytoviral cardiac glycoside DAS-ELISA plant extracts Potato virus Y (PVYNTN) |
title | Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants |
title_full | Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants |
title_fullStr | Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants |
title_full_unstemmed | Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants |
title_short | Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants |
title_sort | plants containing cardiac glycosides showing antiphytoviral activity against potato virus y pvyntn on tobacco plants |
topic | antiphytoviral cardiac glycoside DAS-ELISA plant extracts Potato virus Y (PVYNTN) |
url | https://doi.org/10.24425/jppr.2018.124648 |
work_keys_str_mv | AT siminemamzadehyazdi plantscontainingcardiacglycosidesshowingantiphytoviralactivityagainstpotatovirusypvyntnontobaccoplants AT juliamulabisana plantscontainingcardiacglycosidesshowingantiphytoviralactivityagainstpotatovirusypvyntnontobaccoplants AT gerhardprinsloo plantscontainingcardiacglycosidesshowingantiphytoviralactivityagainstpotatovirusypvyntnontobaccoplants AT michelecloete plantscontainingcardiacglycosidesshowingantiphytoviralactivityagainstpotatovirusypvyntnontobaccoplants AT quentonkritzinger plantscontainingcardiacglycosidesshowingantiphytoviralactivityagainstpotatovirusypvyntnontobaccoplants |