Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants

The tuber necrotic strain of Potato virus Y (PVYNTN) causes widespread disease and has severe negative effects on the growth and yields of plants, especially those of the Solanaceae family. The consequences of residual toxicity and non-biodegradation of synthetic chemicals and pollution of the en...

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Main Authors: Simin Emamzadeh Yazdi, Julia Mulabisana, Gerhard Prinsloo, Michele Cloete, Quenton Kritzinger
Format: Article
Language:English
Published: Polish Academy of Sciences 2018-12-01
Series:Journal of Plant Protection Research
Subjects:
Online Access:https://doi.org/10.24425/jppr.2018.124648
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author Simin Emamzadeh Yazdi
Julia Mulabisana
Gerhard Prinsloo
Michele Cloete
Quenton Kritzinger
author_facet Simin Emamzadeh Yazdi
Julia Mulabisana
Gerhard Prinsloo
Michele Cloete
Quenton Kritzinger
author_sort Simin Emamzadeh Yazdi
collection DOAJ
description The tuber necrotic strain of Potato virus Y (PVYNTN) causes widespread disease and has severe negative effects on the growth and yields of plants, especially those of the Solanaceae family. The consequences of residual toxicity and non-biodegradation of synthetic chemicals and pollution of the environment has led to investigations into new non-toxic and biological treatments to control plant viral diseases. Ethanolic extracts of Bowiea volubilis (bulbs), Cotyledon orbiculata (leaves), Gomphocarpus fruticosus (leaves), Merwilla plumbea (dry and fresh bulbs), Nerium oleander (leaves), and the fruits and leaves of Strophanthus speciosus, were evaluated against PVYNTN in vivo and in vitro. At a concentration of 20 mg · ml−1, ethanolic extracts of Strophanthus speciosus (leaves) and fruits (50 mg · ml−1) significantly reduced the expression of PVYNTN symptoms on tobacco plants in vitro without affecting the normal growth and development of the plant. Similarly, at 50 mg · ml−1, N. oleander, C. orbiculata and B. volubilis (fresh bulbs) and S. speciousus leaves at 20 mg · ml−1 extracts showed significant differences in PVYNTN symptoms in the in vivo experiment. Strophanthus speciosus leaf and fruit extracts showed significant inhibition in the in vitro and in vivo assays and demonstrated that S. speciosus has potential to be used as an antiphytoviral treatment.
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spelling doaj.art-8b4dbb11ef6d418b9532fc6c3dcecdc62022-12-21T19:23:52ZengPolish Academy of SciencesJournal of Plant Protection Research1899-007X1899-007X2018-12-0158439540310.24425/jppr.2018.124648Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plantsSimin Emamzadeh Yazdi0Julia Mulabisana1Gerhard Prinsloo2Michele Cloete3Quenton Kritzinger4Department of Plant and Soil Sciences, University of Pretoria, Pretoria, South AfricaAgricultural Research Council, Vegetable and Ornamental Plants, Roodeplaat, Pretoria, South AfricaDepartment of Agriculture and Animal Health, University of South Africa (UNISA), Florida Campus, South AfricaAgricultural Research Council, Vegetable and Ornamental Plants, Roodeplaat, Pretoria, South AfricaDepartment of Plant and Soil Sciences, University of Pretoria, Pretoria, South AfricaThe tuber necrotic strain of Potato virus Y (PVYNTN) causes widespread disease and has severe negative effects on the growth and yields of plants, especially those of the Solanaceae family. The consequences of residual toxicity and non-biodegradation of synthetic chemicals and pollution of the environment has led to investigations into new non-toxic and biological treatments to control plant viral diseases. Ethanolic extracts of Bowiea volubilis (bulbs), Cotyledon orbiculata (leaves), Gomphocarpus fruticosus (leaves), Merwilla plumbea (dry and fresh bulbs), Nerium oleander (leaves), and the fruits and leaves of Strophanthus speciosus, were evaluated against PVYNTN in vivo and in vitro. At a concentration of 20 mg · ml−1, ethanolic extracts of Strophanthus speciosus (leaves) and fruits (50 mg · ml−1) significantly reduced the expression of PVYNTN symptoms on tobacco plants in vitro without affecting the normal growth and development of the plant. Similarly, at 50 mg · ml−1, N. oleander, C. orbiculata and B. volubilis (fresh bulbs) and S. speciousus leaves at 20 mg · ml−1 extracts showed significant differences in PVYNTN symptoms in the in vivo experiment. Strophanthus speciosus leaf and fruit extracts showed significant inhibition in the in vitro and in vivo assays and demonstrated that S. speciosus has potential to be used as an antiphytoviral treatment.https://doi.org/10.24425/jppr.2018.124648antiphytoviralcardiac glycosideDAS-ELISAplant extractsPotato virus Y (PVYNTN)
spellingShingle Simin Emamzadeh Yazdi
Julia Mulabisana
Gerhard Prinsloo
Michele Cloete
Quenton Kritzinger
Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants
Journal of Plant Protection Research
antiphytoviral
cardiac glycoside
DAS-ELISA
plant extracts
Potato virus Y (PVYNTN)
title Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants
title_full Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants
title_fullStr Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants
title_full_unstemmed Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants
title_short Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants
title_sort plants containing cardiac glycosides showing antiphytoviral activity against potato virus y pvyntn on tobacco plants
topic antiphytoviral
cardiac glycoside
DAS-ELISA
plant extracts
Potato virus Y (PVYNTN)
url https://doi.org/10.24425/jppr.2018.124648
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