Molecular Composition and Biological Activity of a Novel Acetonitrile–Water Extract of Lens Culinaris Medik in Murine Native Cells and Cell Lines Exposed to Different Chemotherapeutics Using Mass Spectrometry
We evaluated the effects of a new extract (70% acetonitrile, 2E0217022196DIPFARMTDA) of Lens culinaris Medik (Terre di Altamura SRL, Altamura BA) to prevent cytotoxic damage from cisplatin, staurosporine, irinotecan, doxorubicin, and the glucocorticoid dexamethasone. The acetonitrile–water extract (...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/4/575 |
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| author | Annamaria Di Turi Marina Antonacci Jacopo Raffaele Dibenedetto Fatima Maqoud Francesco Leonetti Gerardo Centoducati Nicola Colonna Domenico Tricarico |
| author_facet | Annamaria Di Turi Marina Antonacci Jacopo Raffaele Dibenedetto Fatima Maqoud Francesco Leonetti Gerardo Centoducati Nicola Colonna Domenico Tricarico |
| author_sort | Annamaria Di Turi |
| collection | DOAJ |
| description | We evaluated the effects of a new extract (70% acetonitrile, 2E0217022196DIPFARMTDA) of Lens culinaris Medik (Terre di Altamura SRL, Altamura BA) to prevent cytotoxic damage from cisplatin, staurosporine, irinotecan, doxorubicin, and the glucocorticoid dexamethasone. The acetonitrile–water extract (range 0.1–5 mg/mL) was obtained by extracting 10 g of lentil flour with 50 milliliters of the acetonitrile–water extraction mixture in a 70:30 ratio, first for 3 h and then overnight in a shaker at room temperature. The next day, the extract was filtered and passed through a Rotavapor to obtain only the aqueous component and eliminate that with acetonitrile, and then freeze-dried to finally have the powdered extract. In vitro experiments showed that the extract prevented the cytotoxic damage induced by cisplatin, irinotecan, and doxorubicin on HEK293 and SHSY5Y cell lines after 24–96 h. In murine osteoblasts after 24–72 h of incubation time, the extract was cytoprotective against all chemicals. The extract was effective against dexamethasone, leading to synergic cell proliferation in all cell types. In bone marrow cells, the extract is cytoprotective after 72 h against doxorubicin, staurosporine, and dexamethasone. Instead, on muscle fibers, the extract has a synergic effect with chemotherapeutics, increasing cytotoxicity induced by doxorubicin and staurosporine. LC-MS attested to the existence of several phenolic structures in the extract. The most abundant families of compounds were flavonoids (25.7%) and mellitic acid (18%). Thus, the development of this extract could be implemented in the area of research related to the chemoprevention of damage to renal, neuronal, bone marrow cells, and osteoblasts by chemotherapeutics; moreover, it could be used as a reinforcer of cytotoxic action of chemotherapeutics on muscle fibers. |
| first_indexed | 2024-03-11T09:01:01Z |
| format | Article |
| id | doaj.art-8b4f4d282c024b02a604859a42de0cfa |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T09:01:01Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-8b4f4d282c024b02a604859a42de0cfa2023-11-16T19:44:15ZengMDPI AGCells2073-44092023-02-0112457510.3390/cells12040575Molecular Composition and Biological Activity of a Novel Acetonitrile–Water Extract of Lens Culinaris Medik in Murine Native Cells and Cell Lines Exposed to Different Chemotherapeutics Using Mass SpectrometryAnnamaria Di Turi0Marina Antonacci1Jacopo Raffaele Dibenedetto2Fatima Maqoud3Francesco Leonetti4Gerardo Centoducati5Nicola Colonna6Domenico Tricarico7Department of Pharmacy-Pharmaceutical Science, University of Bari “Aldo Moro”, Via Orabona 4, 70125 Bari, ItalyDepartment of Pharmacy-Pharmaceutical Science, University of Bari “Aldo Moro”, Via Orabona 4, 70125 Bari, ItalyDepartment of Pharmacy-Pharmaceutical Science, University of Bari “Aldo Moro”, Via Orabona 4, 70125 Bari, ItalyDepartment of Pharmacy-Pharmaceutical Science, University of Bari “Aldo Moro”, Via Orabona 4, 70125 Bari, ItalyDepartment of Pharmacy-Pharmaceutical Science, University of Bari “Aldo Moro”, Via Orabona 4, 70125 Bari, ItalyDepartment of Medicine Veterinary, University of Bari “Aldo Moro”, Str 62 to Casamassima, Valenzano, 70121 Bari, ItalyTerre di Altamura Srl, 70022 Altamura, ItalyDepartment of Pharmacy-Pharmaceutical Science, University of Bari “Aldo Moro”, Via Orabona 4, 70125 Bari, ItalyWe evaluated the effects of a new extract (70% acetonitrile, 2E0217022196DIPFARMTDA) of Lens culinaris Medik (Terre di Altamura SRL, Altamura BA) to prevent cytotoxic damage from cisplatin, staurosporine, irinotecan, doxorubicin, and the glucocorticoid dexamethasone. The acetonitrile–water extract (range 0.1–5 mg/mL) was obtained by extracting 10 g of lentil flour with 50 milliliters of the acetonitrile–water extraction mixture in a 70:30 ratio, first for 3 h and then overnight in a shaker at room temperature. The next day, the extract was filtered and passed through a Rotavapor to obtain only the aqueous component and eliminate that with acetonitrile, and then freeze-dried to finally have the powdered extract. In vitro experiments showed that the extract prevented the cytotoxic damage induced by cisplatin, irinotecan, and doxorubicin on HEK293 and SHSY5Y cell lines after 24–96 h. In murine osteoblasts after 24–72 h of incubation time, the extract was cytoprotective against all chemicals. The extract was effective against dexamethasone, leading to synergic cell proliferation in all cell types. In bone marrow cells, the extract is cytoprotective after 72 h against doxorubicin, staurosporine, and dexamethasone. Instead, on muscle fibers, the extract has a synergic effect with chemotherapeutics, increasing cytotoxicity induced by doxorubicin and staurosporine. LC-MS attested to the existence of several phenolic structures in the extract. The most abundant families of compounds were flavonoids (25.7%) and mellitic acid (18%). Thus, the development of this extract could be implemented in the area of research related to the chemoprevention of damage to renal, neuronal, bone marrow cells, and osteoblasts by chemotherapeutics; moreover, it could be used as a reinforcer of cytotoxic action of chemotherapeutics on muscle fibers.https://www.mdpi.com/2073-4409/12/4/575Lens culinarischemopreventivecytotoxicityprimary murine cellsflavonoidsliquid chromatography–mass spectrometry |
| spellingShingle | Annamaria Di Turi Marina Antonacci Jacopo Raffaele Dibenedetto Fatima Maqoud Francesco Leonetti Gerardo Centoducati Nicola Colonna Domenico Tricarico Molecular Composition and Biological Activity of a Novel Acetonitrile–Water Extract of Lens Culinaris Medik in Murine Native Cells and Cell Lines Exposed to Different Chemotherapeutics Using Mass Spectrometry Cells Lens culinaris chemopreventive cytotoxicity primary murine cells flavonoids liquid chromatography–mass spectrometry |
| title | Molecular Composition and Biological Activity of a Novel Acetonitrile–Water Extract of Lens Culinaris Medik in Murine Native Cells and Cell Lines Exposed to Different Chemotherapeutics Using Mass Spectrometry |
| title_full | Molecular Composition and Biological Activity of a Novel Acetonitrile–Water Extract of Lens Culinaris Medik in Murine Native Cells and Cell Lines Exposed to Different Chemotherapeutics Using Mass Spectrometry |
| title_fullStr | Molecular Composition and Biological Activity of a Novel Acetonitrile–Water Extract of Lens Culinaris Medik in Murine Native Cells and Cell Lines Exposed to Different Chemotherapeutics Using Mass Spectrometry |
| title_full_unstemmed | Molecular Composition and Biological Activity of a Novel Acetonitrile–Water Extract of Lens Culinaris Medik in Murine Native Cells and Cell Lines Exposed to Different Chemotherapeutics Using Mass Spectrometry |
| title_short | Molecular Composition and Biological Activity of a Novel Acetonitrile–Water Extract of Lens Culinaris Medik in Murine Native Cells and Cell Lines Exposed to Different Chemotherapeutics Using Mass Spectrometry |
| title_sort | molecular composition and biological activity of a novel acetonitrile water extract of lens culinaris medik in murine native cells and cell lines exposed to different chemotherapeutics using mass spectrometry |
| topic | Lens culinaris chemopreventive cytotoxicity primary murine cells flavonoids liquid chromatography–mass spectrometry |
| url | https://www.mdpi.com/2073-4409/12/4/575 |
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