A Bright Future for Fluorescence Imaging of Fungi in Living Hosts
Traditional <i>in vivo</i> investigation of fungal infection and new antifungal therapies in mouse models is usually carried out using post mortem methodologies. However, biomedical imaging techniques focusing on non-invasive techniques using bioluminescent and fluorescent proteins have...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-04-01
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| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/5/2/29 |
| _version_ | 1818933420322979840 |
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| author | Ambre F. Chapuis Elizabeth R. Ballou Donna M. MacCallum |
| author_facet | Ambre F. Chapuis Elizabeth R. Ballou Donna M. MacCallum |
| author_sort | Ambre F. Chapuis |
| collection | DOAJ |
| description | Traditional <i>in vivo</i> investigation of fungal infection and new antifungal therapies in mouse models is usually carried out using post mortem methodologies. However, biomedical imaging techniques focusing on non-invasive techniques using bioluminescent and fluorescent proteins have become valuable tools. These new techniques address ethical concerns as they allow reduction in the number of animals required to evaluate new antifungal therapies. They also allow better understanding of the growth and spread of the pathogen during infection. In this review, we concentrate on imaging technologies using different fungal reporter proteins. We discuss the advantages and limitations of these different reporters and compare the efficacy of bioluminescent and fluorescent proteins for fungal research. |
| first_indexed | 2024-12-20T04:48:06Z |
| format | Article |
| id | doaj.art-8b505579f4ca40928183310fa28b7694 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-12-20T04:48:06Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-8b505579f4ca40928183310fa28b76942022-12-21T19:52:56ZengMDPI AGJournal of Fungi2309-608X2019-04-01522910.3390/jof5020029jof5020029A Bright Future for Fluorescence Imaging of Fungi in Living HostsAmbre F. Chapuis0Elizabeth R. Ballou1Donna M. MacCallum2MRC Centre for Medical Mycology at the University of Aberdeen, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UKInstitute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham B15 2TT, UKMRC Centre for Medical Mycology at the University of Aberdeen, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UKTraditional <i>in vivo</i> investigation of fungal infection and new antifungal therapies in mouse models is usually carried out using post mortem methodologies. However, biomedical imaging techniques focusing on non-invasive techniques using bioluminescent and fluorescent proteins have become valuable tools. These new techniques address ethical concerns as they allow reduction in the number of animals required to evaluate new antifungal therapies. They also allow better understanding of the growth and spread of the pathogen during infection. In this review, we concentrate on imaging technologies using different fungal reporter proteins. We discuss the advantages and limitations of these different reporters and compare the efficacy of bioluminescent and fluorescent proteins for fungal research.https://www.mdpi.com/2309-608X/5/2/29fluorescent reportersbioluminescenceiRFPlive imaginginfection models3Rs |
| spellingShingle | Ambre F. Chapuis Elizabeth R. Ballou Donna M. MacCallum A Bright Future for Fluorescence Imaging of Fungi in Living Hosts Journal of Fungi fluorescent reporters bioluminescence iRFP live imaging infection models 3Rs |
| title | A Bright Future for Fluorescence Imaging of Fungi in Living Hosts |
| title_full | A Bright Future for Fluorescence Imaging of Fungi in Living Hosts |
| title_fullStr | A Bright Future for Fluorescence Imaging of Fungi in Living Hosts |
| title_full_unstemmed | A Bright Future for Fluorescence Imaging of Fungi in Living Hosts |
| title_short | A Bright Future for Fluorescence Imaging of Fungi in Living Hosts |
| title_sort | bright future for fluorescence imaging of fungi in living hosts |
| topic | fluorescent reporters bioluminescence iRFP live imaging infection models 3Rs |
| url | https://www.mdpi.com/2309-608X/5/2/29 |
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