Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes
The present study investigated the effect of autophagy induction and cathepsin B (CTSB) inhibition on developmental competence of poor quality oocytes. Bovine cumulus oocyte complexes (COCs) were classified as good or poor according to their morphology. Autophagy activity was detected in good and po...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2019-12-01
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| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/66/1/66_2019-123/_pdf/-char/en |
| _version_ | 1797615255868669952 |
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| author | Jianye LI Ahmed Zaky BALBOULA Mansour ABOELENAIN Takashi FUJII Satoru MORIYASU Hanako BAI Manabu KAWAHARA Masashi TAKAHASHI |
| author_facet | Jianye LI Ahmed Zaky BALBOULA Mansour ABOELENAIN Takashi FUJII Satoru MORIYASU Hanako BAI Manabu KAWAHARA Masashi TAKAHASHI |
| author_sort | Jianye LI |
| collection | DOAJ |
| description | The present study investigated the effect of autophagy induction and cathepsin B (CTSB) inhibition on developmental competence of poor quality oocytes. Bovine cumulus oocyte complexes (COCs) were classified as good or poor according to their morphology. Autophagy activity was detected in good and poor germinal vesicle (GV) oocytes. Then E-64, a CTSB inhibitor, rapamycin (Rapa), an autophagy inducer, and combined administration was achieved during invitro maturation (IVM) of poor quality COCs followed by detection of autophagy activity. In the next experiment, E-64, Rapa, and E64 + Rapa, were added during IVM to good and poor quality COCs followed by invitro fertilization and culture for 8 days to investigate whether inhibition of CTSB and/or induction of autophagy improve embryonic development and quality. Autophagy activity was significantly lower in poor quality GV oocytes than in good quality ones. E-64, Rapa and E-64 + Rapa treatment during IVM significantly increased autophagy activity in poor quality oocytes. Addition of Rapa in good quality COCs did not increase the blastocyst rate, whereas E-64 increased the blastocyst rate and total cell number (TCN) with decreasing TUNEL-positive cells. In contrast, Rapa treatment in poor quality COCs significantly increased the blastocyst rate and TCN with decreasing TUNEL-positive cells. These results indicate oocyte quality has different responses to intracellular autophagy induction and CTSB activity control by potential autophagy and catabolic status, however, synergetic effect of autophagy induction and CTSB inhibition can increase developmental competence of both good and poor quality COCs, especially rescue effect in poor quality COCs. |
| first_indexed | 2024-03-11T07:23:42Z |
| format | Article |
| id | doaj.art-8b508da9038d4cb8b462405f4f0e0b37 |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T07:23:42Z |
| publishDate | 2019-12-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-8b508da9038d4cb8b462405f4f0e0b372023-11-17T07:44:30ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002019-12-01661839110.1262/jrd.2019-123jrdEffect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytesJianye LI0Ahmed Zaky BALBOULA1Mansour ABOELENAIN2Takashi FUJII3Satoru MORIYASU4Hanako BAI5Manabu KAWAHARA6Masashi TAKAHASHI7Laboratory of Animal Genetics and Reproduction, Department of Animal Science, Graduate School of Agriculture, Hokkaido University, Hokkaido 060-8589, JapanAnimal Sciences Research Center, College of Agriculture, Food & Natural Resources, University of Missouri, Columbia, MO 65211, USADepartment of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptAnimal Research Center, Hokkaido Research Organization, Hokkaido 081-0038, JapanAnimal Research Center, Hokkaido Research Organization, Hokkaido 081-0038, JapanLaboratory of Animal Genetics and Reproduction, Department of Animal Science, Graduate School of Agriculture, Hokkaido University, Hokkaido 060-8589, JapanLaboratory of Animal Genetics and Reproduction, Department of Animal Science, Graduate School of Agriculture, Hokkaido University, Hokkaido 060-8589, JapanResearch Faculty of Agriculture, Hokkaido University, Hokkaido 060-8589, JapanThe present study investigated the effect of autophagy induction and cathepsin B (CTSB) inhibition on developmental competence of poor quality oocytes. Bovine cumulus oocyte complexes (COCs) were classified as good or poor according to their morphology. Autophagy activity was detected in good and poor germinal vesicle (GV) oocytes. Then E-64, a CTSB inhibitor, rapamycin (Rapa), an autophagy inducer, and combined administration was achieved during invitro maturation (IVM) of poor quality COCs followed by detection of autophagy activity. In the next experiment, E-64, Rapa, and E64 + Rapa, were added during IVM to good and poor quality COCs followed by invitro fertilization and culture for 8 days to investigate whether inhibition of CTSB and/or induction of autophagy improve embryonic development and quality. Autophagy activity was significantly lower in poor quality GV oocytes than in good quality ones. E-64, Rapa and E-64 + Rapa treatment during IVM significantly increased autophagy activity in poor quality oocytes. Addition of Rapa in good quality COCs did not increase the blastocyst rate, whereas E-64 increased the blastocyst rate and total cell number (TCN) with decreasing TUNEL-positive cells. In contrast, Rapa treatment in poor quality COCs significantly increased the blastocyst rate and TCN with decreasing TUNEL-positive cells. These results indicate oocyte quality has different responses to intracellular autophagy induction and CTSB activity control by potential autophagy and catabolic status, however, synergetic effect of autophagy induction and CTSB inhibition can increase developmental competence of both good and poor quality COCs, especially rescue effect in poor quality COCs.https://www.jstage.jst.go.jp/article/jrd/66/1/66_2019-123/_pdf/-char/enautophagycathepsin bcowdevelopmental competenceoocyte quality |
| spellingShingle | Jianye LI Ahmed Zaky BALBOULA Mansour ABOELENAIN Takashi FUJII Satoru MORIYASU Hanako BAI Manabu KAWAHARA Masashi TAKAHASHI Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes The Journal of Reproduction and Development autophagy cathepsin b cow developmental competence oocyte quality |
| title | Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes |
| title_full | Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes |
| title_fullStr | Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes |
| title_full_unstemmed | Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes |
| title_short | Effect of autophagy induction and cathepsin B inhibition on developmental competence of poor quality bovine oocytes |
| title_sort | effect of autophagy induction and cathepsin b inhibition on developmental competence of poor quality bovine oocytes |
| topic | autophagy cathepsin b cow developmental competence oocyte quality |
| url | https://www.jstage.jst.go.jp/article/jrd/66/1/66_2019-123/_pdf/-char/en |
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