Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments
Abstract Imaging the structure and observing the dynamics of isolated proteins using single-particle X-ray diffractive imaging (SPI) is one of the potential applications of X-ray free-electron lasers (XFELs). Currently, SPI experiments on isolated proteins are limited by three factors: low signal st...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-54605-9 |
| _version_ | 1797275080934293504 |
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| author | Tej Varma Yenupuri Safi Rafie-Zinedine Lena Worbs Michael Heymann Joachim Schulz Johan Bielecki Filipe R. N. C. Maia |
| author_facet | Tej Varma Yenupuri Safi Rafie-Zinedine Lena Worbs Michael Heymann Joachim Schulz Johan Bielecki Filipe R. N. C. Maia |
| author_sort | Tej Varma Yenupuri |
| collection | DOAJ |
| description | Abstract Imaging the structure and observing the dynamics of isolated proteins using single-particle X-ray diffractive imaging (SPI) is one of the potential applications of X-ray free-electron lasers (XFELs). Currently, SPI experiments on isolated proteins are limited by three factors: low signal strength, limited data and high background from gas scattering. The last two factors are largely due to the shortcomings of the aerosol sample delivery methods in use. Here we present our modified electrospray ionization (ESI) source, which we dubbed helium-ESI (He-ESI). With it, we increased particle delivery into the interaction region by a factor of 10, for 26 nm-sized biological particles, and decreased the gas load in the interaction chamber corresponding to an 80% reduction in gas scattering when compared to the original ESI. These improvements have the potential to significantly increase the quality and quantity of SPI diffraction patterns in future experiments using He-ESI, resulting in higher-resolution structures. |
| first_indexed | 2024-03-07T15:09:15Z |
| format | Article |
| id | doaj.art-8b520020752e4fb8a0471624370323ed |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:09:15Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-8b520020752e4fb8a0471624370323ed2024-03-05T18:46:17ZengNature PortfolioScientific Reports2045-23222024-02-011411810.1038/s41598-024-54605-9Helium-electrospray improves sample delivery in X-ray single-particle imaging experimentsTej Varma Yenupuri0Safi Rafie-Zinedine1Lena Worbs2Michael Heymann3Joachim Schulz4Johan Bielecki5Filipe R. N. C. Maia6Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala UniversityEuropean XFELLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala UniversityInstitute of Biomaterials and Biomolecular Systems, University of StuttgartEuropean XFELEuropean XFELLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala UniversityAbstract Imaging the structure and observing the dynamics of isolated proteins using single-particle X-ray diffractive imaging (SPI) is one of the potential applications of X-ray free-electron lasers (XFELs). Currently, SPI experiments on isolated proteins are limited by three factors: low signal strength, limited data and high background from gas scattering. The last two factors are largely due to the shortcomings of the aerosol sample delivery methods in use. Here we present our modified electrospray ionization (ESI) source, which we dubbed helium-ESI (He-ESI). With it, we increased particle delivery into the interaction region by a factor of 10, for 26 nm-sized biological particles, and decreased the gas load in the interaction chamber corresponding to an 80% reduction in gas scattering when compared to the original ESI. These improvements have the potential to significantly increase the quality and quantity of SPI diffraction patterns in future experiments using He-ESI, resulting in higher-resolution structures.https://doi.org/10.1038/s41598-024-54605-9 |
| spellingShingle | Tej Varma Yenupuri Safi Rafie-Zinedine Lena Worbs Michael Heymann Joachim Schulz Johan Bielecki Filipe R. N. C. Maia Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments Scientific Reports |
| title | Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments |
| title_full | Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments |
| title_fullStr | Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments |
| title_full_unstemmed | Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments |
| title_short | Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments |
| title_sort | helium electrospray improves sample delivery in x ray single particle imaging experiments |
| url | https://doi.org/10.1038/s41598-024-54605-9 |
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