First report of co-infections of Marek's disease virus and chicken infectious anaemia virus in poultry flocks in Nigeria
Marek's disease (MD) and chicken infectious anaemia (CIA) are viral immunosuppressive diseases of poultry caused by the MD virus (MDV) and CIA virus (CIAV) respectively. Despite vaccination against MD, the incidence of the disease in vaccinated poultry flocks in Nigeria persists. However, under...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Veterinary and Animal Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2451943X24000061 |
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| author | Adeyinka J. Adedeji Ismail Shittu Olatunde B. Akanbi Olayinka O. Asala Jolly A. Adole Philip A. Okewole Gabriel O. Ijale Dennis Kabantiyok Felix Idoko Johnson J. Shallmizhili Paul A. Abdu Shedrach B. Pewan |
| author_facet | Adeyinka J. Adedeji Ismail Shittu Olatunde B. Akanbi Olayinka O. Asala Jolly A. Adole Philip A. Okewole Gabriel O. Ijale Dennis Kabantiyok Felix Idoko Johnson J. Shallmizhili Paul A. Abdu Shedrach B. Pewan |
| author_sort | Adeyinka J. Adedeji |
| collection | DOAJ |
| description | Marek's disease (MD) and chicken infectious anaemia (CIA) are viral immunosuppressive diseases of poultry caused by the MD virus (MDV) and CIA virus (CIAV) respectively. Despite vaccination against MD, the incidence of the disease in vaccinated poultry flocks in Nigeria persists. However, underlying factors like co-infection with CIAV have not been investigated in the country. This study was designed to investigate possible co-infections of MDV and CIAV in poultry flocks in Nigeria. In 2016, tumorous tissue samples were collected from suspected cases of MD at necropsy in Jos, Plateau State, Nigeria. The samples collected were fixed in formalin for histopathological examination, genomic DNA was extracted from a second part and analysed by polymerase chain reaction (PCR), targeting the meq and VP1 genes of the MDV and CIAV, respectively. The histology results revealed that the cutaneous and proventricular lymphomas were characterized by large numbers of mononuclear cellular infiltrates admixed with heterophils. The PCR results revealed that MDV was detected in 66.7% (16/24), CIAV in 45.8% (11/24), and co-infections of MDV and CIAV were detected in 45.8% (11/24) of the samples analysed. In addition, co-infections of MD and CIA were recorded in 100% (6/6) and 27.7% (5/18) of broilers and layer/pullet’ samples respectively. Phylogenetic analysis of the meq gene sequences revealed that the Nigerian MDV clusters with very virulent MDV from Egypt and Italy. While, CIAV sequences were genotype II and genotype III and clustered with CIAVs from Cameroon and China. This is the first report of co-infections of MD and CIA in Nigeria. |
| first_indexed | 2024-03-07T14:28:35Z |
| format | Article |
| id | doaj.art-8b53624db7b84b6ead9e60e360c61e8e |
| institution | Directory Open Access Journal |
| issn | 2451-943X |
| language | English |
| last_indexed | 2024-03-07T14:28:35Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Veterinary and Animal Science |
| spelling | doaj.art-8b53624db7b84b6ead9e60e360c61e8e2024-03-06T05:28:25ZengElsevierVeterinary and Animal Science2451-943X2024-03-0123100339First report of co-infections of Marek's disease virus and chicken infectious anaemia virus in poultry flocks in NigeriaAdeyinka J. Adedeji0Ismail Shittu1Olatunde B. Akanbi2Olayinka O. Asala3Jolly A. Adole4Philip A. Okewole5Gabriel O. Ijale6Dennis Kabantiyok7Felix Idoko8Johnson J. Shallmizhili9Paul A. Abdu10Shedrach B. Pewan11National Veterinary Research Institute Vom, Nigeria; Corresponding author.National Veterinary Research Institute Vom, NigeriaDepartment of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ilorin, NigeriaNational Veterinary Research Institute Vom, NigeriaNational Veterinary Research Institute Vom, NigeriaNational Veterinary Research Institute Vom, NigeriaFederal Ministry of Agriculture and Rural Development, Abuja, NigeriaNational Veterinary Research Institute Vom, NigeriaNational Veterinary Research Institute Vom, NigeriaFaculty of Veterinary Medicine, Ahmadu Bello University Zaria, NigeriaFaculty of Veterinary Medicine, Ahmadu Bello University Zaria, NigeriaNational Veterinary Research Institute Vom, NigeriaMarek's disease (MD) and chicken infectious anaemia (CIA) are viral immunosuppressive diseases of poultry caused by the MD virus (MDV) and CIA virus (CIAV) respectively. Despite vaccination against MD, the incidence of the disease in vaccinated poultry flocks in Nigeria persists. However, underlying factors like co-infection with CIAV have not been investigated in the country. This study was designed to investigate possible co-infections of MDV and CIAV in poultry flocks in Nigeria. In 2016, tumorous tissue samples were collected from suspected cases of MD at necropsy in Jos, Plateau State, Nigeria. The samples collected were fixed in formalin for histopathological examination, genomic DNA was extracted from a second part and analysed by polymerase chain reaction (PCR), targeting the meq and VP1 genes of the MDV and CIAV, respectively. The histology results revealed that the cutaneous and proventricular lymphomas were characterized by large numbers of mononuclear cellular infiltrates admixed with heterophils. The PCR results revealed that MDV was detected in 66.7% (16/24), CIAV in 45.8% (11/24), and co-infections of MDV and CIAV were detected in 45.8% (11/24) of the samples analysed. In addition, co-infections of MD and CIA were recorded in 100% (6/6) and 27.7% (5/18) of broilers and layer/pullet’ samples respectively. Phylogenetic analysis of the meq gene sequences revealed that the Nigerian MDV clusters with very virulent MDV from Egypt and Italy. While, CIAV sequences were genotype II and genotype III and clustered with CIAVs from Cameroon and China. This is the first report of co-infections of MD and CIA in Nigeria.http://www.sciencedirect.com/science/article/pii/S2451943X24000061Chicken infectious anaemia virusCo-infectionImmunosuppressionMarek's disease virusNigeria |
| spellingShingle | Adeyinka J. Adedeji Ismail Shittu Olatunde B. Akanbi Olayinka O. Asala Jolly A. Adole Philip A. Okewole Gabriel O. Ijale Dennis Kabantiyok Felix Idoko Johnson J. Shallmizhili Paul A. Abdu Shedrach B. Pewan First report of co-infections of Marek's disease virus and chicken infectious anaemia virus in poultry flocks in Nigeria Veterinary and Animal Science Chicken infectious anaemia virus Co-infection Immunosuppression Marek's disease virus Nigeria |
| title | First report of co-infections of Marek's disease virus and chicken infectious anaemia virus in poultry flocks in Nigeria |
| title_full | First report of co-infections of Marek's disease virus and chicken infectious anaemia virus in poultry flocks in Nigeria |
| title_fullStr | First report of co-infections of Marek's disease virus and chicken infectious anaemia virus in poultry flocks in Nigeria |
| title_full_unstemmed | First report of co-infections of Marek's disease virus and chicken infectious anaemia virus in poultry flocks in Nigeria |
| title_short | First report of co-infections of Marek's disease virus and chicken infectious anaemia virus in poultry flocks in Nigeria |
| title_sort | first report of co infections of marek s disease virus and chicken infectious anaemia virus in poultry flocks in nigeria |
| topic | Chicken infectious anaemia virus Co-infection Immunosuppression Marek's disease virus Nigeria |
| url | http://www.sciencedirect.com/science/article/pii/S2451943X24000061 |
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