Amplification of urea detection based on pH-sensitive liposomes
Background: This study aimed to develop an amplification method of urea detection based on pH-sensitive liposomes. Results: The urease covalently immobilized on the magnetic particles and the pH-sensitive liposomes encapsulating ferricyanide were added to the cyclic-voltammeter cell solution where u...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Electronic Journal of Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345821000208 |
| _version_ | 1818864620948946944 |
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| author | Min Kyeong Kang Jin-Won Park |
| author_facet | Min Kyeong Kang Jin-Won Park |
| author_sort | Min Kyeong Kang |
| collection | DOAJ |
| description | Background: This study aimed to develop an amplification method of urea detection based on pH-sensitive liposomes. Results: The urease covalently immobilized on the magnetic particles and the pH-sensitive liposomes encapsulating ferricyanide were added to the cyclic-voltammeter cell solution where urea was distributed. The conversion of urea into carbonic acid seemed to induce a pH decrease that caused a reduction in the electrostatic repulsion between the headgroups of weakly acidic 1,2-dipalmitoyl-sn-glycero-3-succinate. The reduction induced the liposomes to release potassium ferricyanide that was encapsulated inside. The effects of urea concentration and pH value were investigated. A specific concentration (0.5 mg/mL) of the urea solution was set to observe the response. The activity of urease was reversible with respect to the pH change between 7 and 5. The sensitivity of this detection was almost identical to the comparable techniques such as an enzyme-linked immunosorbent assay and a field-effect transistor. Conclusions: In summary, the methodology developed in this study was feasible as a portable, rapid, and sensitive method.How to cite: Kang MK, Park J-W. Amplification of urea detection based on pH-sensitive liposomes. Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.04.005 |
| first_indexed | 2024-12-19T10:34:33Z |
| format | Article |
| id | doaj.art-8b550d786f04497b8f3e42cbb8871fba |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-19T10:34:33Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-8b550d786f04497b8f3e42cbb8871fba2022-12-21T20:25:39ZengElsevierElectronic Journal of Biotechnology0717-34582021-07-01523034Amplification of urea detection based on pH-sensitive liposomesMin Kyeong Kang0Jin-Won Park1Department of Chemical and Biomolecular Engineering, College of Energy and Biotechnology, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, Republic of KoreaCorresponding author.; Department of Chemical and Biomolecular Engineering, College of Energy and Biotechnology, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, Republic of KoreaBackground: This study aimed to develop an amplification method of urea detection based on pH-sensitive liposomes. Results: The urease covalently immobilized on the magnetic particles and the pH-sensitive liposomes encapsulating ferricyanide were added to the cyclic-voltammeter cell solution where urea was distributed. The conversion of urea into carbonic acid seemed to induce a pH decrease that caused a reduction in the electrostatic repulsion between the headgroups of weakly acidic 1,2-dipalmitoyl-sn-glycero-3-succinate. The reduction induced the liposomes to release potassium ferricyanide that was encapsulated inside. The effects of urea concentration and pH value were investigated. A specific concentration (0.5 mg/mL) of the urea solution was set to observe the response. The activity of urease was reversible with respect to the pH change between 7 and 5. The sensitivity of this detection was almost identical to the comparable techniques such as an enzyme-linked immunosorbent assay and a field-effect transistor. Conclusions: In summary, the methodology developed in this study was feasible as a portable, rapid, and sensitive method.How to cite: Kang MK, Park J-W. Amplification of urea detection based on pH-sensitive liposomes. Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.04.005http://www.sciencedirect.com/science/article/pii/S0717345821000208Urea detectionCyclic voltammetrypH-sensitive liposomeImmobilized urease |
| spellingShingle | Min Kyeong Kang Jin-Won Park Amplification of urea detection based on pH-sensitive liposomes Electronic Journal of Biotechnology Urea detection Cyclic voltammetry pH-sensitive liposome Immobilized urease |
| title | Amplification of urea detection based on pH-sensitive liposomes |
| title_full | Amplification of urea detection based on pH-sensitive liposomes |
| title_fullStr | Amplification of urea detection based on pH-sensitive liposomes |
| title_full_unstemmed | Amplification of urea detection based on pH-sensitive liposomes |
| title_short | Amplification of urea detection based on pH-sensitive liposomes |
| title_sort | amplification of urea detection based on ph sensitive liposomes |
| topic | Urea detection Cyclic voltammetry pH-sensitive liposome Immobilized urease |
| url | http://www.sciencedirect.com/science/article/pii/S0717345821000208 |
| work_keys_str_mv | AT minkyeongkang amplificationofureadetectionbasedonphsensitiveliposomes AT jinwonpark amplificationofureadetectionbasedonphsensitiveliposomes |