Wu Shan Shen Cha (<i>Malus asiatica</i> Nakai. Leaves)-Derived Flavonoids Alleviate Alcohol-Induced Gastric Injury in Mice via an Anti-Oxidative Mechanism
Wu Shan Shen Cha is the leaf of <i>Malus asiatica</i> Nakai., a special type of tea that is consumed in the same way as green tea. To study the effect of Wu Shan Shen Cha-derived flavonoids (WSSCF) on lesions in the stomach, a 15% hydrochloric acid−95% ethanol (volume ratio 4:6...
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MDPI AG
2019-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/9/5/169 |
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| author | Bihui Liu Chengfeng Zhang Jing Zhang Xin Zhao |
| author_facet | Bihui Liu Chengfeng Zhang Jing Zhang Xin Zhao |
| author_sort | Bihui Liu |
| collection | DOAJ |
| description | Wu Shan Shen Cha is the leaf of <i>Malus asiatica</i> Nakai., a special type of tea that is consumed in the same way as green tea. To study the effect of Wu Shan Shen Cha-derived flavonoids (WSSCF) on lesions in the stomach, a 15% hydrochloric acid−95% ethanol (volume ratio 4:6) solution was used to induce gastric injury in mice. The degree of gastric injury was assessed using tissue specimens, and the effects of WSSCF on the serum levels of antioxidant enzymes were investigated. The results showed that WSSCF could alleviate the damage of the gastric mucosa and gastric wall caused by the hydrochloric acid−ethanol solution, decrease the tissue and serum levels of malondialdehyde (MDA) in mice with gastric injury, and increase the serum levels of superoxide dismutase (SOD) and glutathione (GSH). The results of quantitative polymerase chain reaction (qPCR) showed that WSSCF could increase the mRNA expression of Mn-SOD, Cu/Zn-SOD, catalase (CAT), endothelial nitric oxide synthase (eNOS), and neuronal nitric oxide synthase (nNOS) in tissue specimens from mice with gastric injury and decrease the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). At the same time, the results of the high concentration of WSSCF (WSSCFH) group were closer to those of the drug (ranitidine) treatment group. Wu Shan Shen Cha-derived flavonoids had a good antioxidant effect, so as to play a preventive role in alcoholic gastric injury. |
| first_indexed | 2024-12-21T09:50:20Z |
| format | Article |
| id | doaj.art-8b5793f299aa40aab24bf6da358a4918 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-21T09:50:20Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-8b5793f299aa40aab24bf6da358a49182022-12-21T19:08:12ZengMDPI AGBiomolecules2218-273X2019-05-019516910.3390/biom9050169biom9050169Wu Shan Shen Cha (<i>Malus asiatica</i> Nakai. Leaves)-Derived Flavonoids Alleviate Alcohol-Induced Gastric Injury in Mice via an Anti-Oxidative MechanismBihui Liu0Chengfeng Zhang1Jing Zhang2Xin Zhao3Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaBiological and Chemical Engineering Collage, Chongqing University of Education, Chongqing 400067, ChinaEnvironment and Quality Inspection College, Chongqing Chemical Industry Vocational College, Chongqing 401228, ChinaChongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, ChinaWu Shan Shen Cha is the leaf of <i>Malus asiatica</i> Nakai., a special type of tea that is consumed in the same way as green tea. To study the effect of Wu Shan Shen Cha-derived flavonoids (WSSCF) on lesions in the stomach, a 15% hydrochloric acid−95% ethanol (volume ratio 4:6) solution was used to induce gastric injury in mice. The degree of gastric injury was assessed using tissue specimens, and the effects of WSSCF on the serum levels of antioxidant enzymes were investigated. The results showed that WSSCF could alleviate the damage of the gastric mucosa and gastric wall caused by the hydrochloric acid−ethanol solution, decrease the tissue and serum levels of malondialdehyde (MDA) in mice with gastric injury, and increase the serum levels of superoxide dismutase (SOD) and glutathione (GSH). The results of quantitative polymerase chain reaction (qPCR) showed that WSSCF could increase the mRNA expression of Mn-SOD, Cu/Zn-SOD, catalase (CAT), endothelial nitric oxide synthase (eNOS), and neuronal nitric oxide synthase (nNOS) in tissue specimens from mice with gastric injury and decrease the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). At the same time, the results of the high concentration of WSSCF (WSSCFH) group were closer to those of the drug (ranitidine) treatment group. Wu Shan Shen Cha-derived flavonoids had a good antioxidant effect, so as to play a preventive role in alcoholic gastric injury.https://www.mdpi.com/2218-273X/9/5/169<i>Malus asiatica</i> Nakai.flavonoidalcoholic gastric injurymRNA expressionoxidation |
| spellingShingle | Bihui Liu Chengfeng Zhang Jing Zhang Xin Zhao Wu Shan Shen Cha (<i>Malus asiatica</i> Nakai. Leaves)-Derived Flavonoids Alleviate Alcohol-Induced Gastric Injury in Mice via an Anti-Oxidative Mechanism Biomolecules <i>Malus asiatica</i> Nakai. flavonoid alcoholic gastric injury mRNA expression oxidation |
| title | Wu Shan Shen Cha (<i>Malus asiatica</i> Nakai. Leaves)-Derived Flavonoids Alleviate Alcohol-Induced Gastric Injury in Mice via an Anti-Oxidative Mechanism |
| title_full | Wu Shan Shen Cha (<i>Malus asiatica</i> Nakai. Leaves)-Derived Flavonoids Alleviate Alcohol-Induced Gastric Injury in Mice via an Anti-Oxidative Mechanism |
| title_fullStr | Wu Shan Shen Cha (<i>Malus asiatica</i> Nakai. Leaves)-Derived Flavonoids Alleviate Alcohol-Induced Gastric Injury in Mice via an Anti-Oxidative Mechanism |
| title_full_unstemmed | Wu Shan Shen Cha (<i>Malus asiatica</i> Nakai. Leaves)-Derived Flavonoids Alleviate Alcohol-Induced Gastric Injury in Mice via an Anti-Oxidative Mechanism |
| title_short | Wu Shan Shen Cha (<i>Malus asiatica</i> Nakai. Leaves)-Derived Flavonoids Alleviate Alcohol-Induced Gastric Injury in Mice via an Anti-Oxidative Mechanism |
| title_sort | wu shan shen cha i malus asiatica i nakai leaves derived flavonoids alleviate alcohol induced gastric injury in mice via an anti oxidative mechanism |
| topic | <i>Malus asiatica</i> Nakai. flavonoid alcoholic gastric injury mRNA expression oxidation |
| url | https://www.mdpi.com/2218-273X/9/5/169 |
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