Study of compartmentalization in the visna clinical form of small ruminant lentivirus infection in sheep
<p>Abstract</p> <p>Background</p> <p>A central nervous system (CNS) disease outbreak caused by small ruminant lentiviruses (SRLV) has triggered interest in Spain due to the rapid onset of clinical signs and relevant production losses. In a previous study on this outbrea...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-01-01
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| Series: | BMC Veterinary Research |
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| Online Access: | http://www.biomedcentral.com/1746-6148/8/8 |
| _version_ | 1819040188778676224 |
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| author | Ramírez Hugo Reina Ramsés Bertolotti Luigi Cenoz Amaia Hernández Mirna-Margarita San Román Beatriz Glaria Idoia de Andrés Ximena Crespo Helena Jáuregui Paula Benavides Julio Polledo Laura Pérez Valentín García-Marín Juan F Rosati Sergio Amorena Beatriz de Andrés Damián |
| author_facet | Ramírez Hugo Reina Ramsés Bertolotti Luigi Cenoz Amaia Hernández Mirna-Margarita San Román Beatriz Glaria Idoia de Andrés Ximena Crespo Helena Jáuregui Paula Benavides Julio Polledo Laura Pérez Valentín García-Marín Juan F Rosati Sergio Amorena Beatriz de Andrés Damián |
| author_sort | Ramírez Hugo |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>A central nervous system (CNS) disease outbreak caused by small ruminant lentiviruses (SRLV) has triggered interest in Spain due to the rapid onset of clinical signs and relevant production losses. In a previous study on this outbreak, the role of LTR in tropism was unclear and <it>env </it>encoded sequences, likely involved in tropism, were not investigated. This study aimed to analyze heterogeneity of SRLV Env regions - TM amino terminal and SU V4, C4 and V5 segments - in order to assess virus compartmentalization in CNS.</p> <p>Results</p> <p>Eight Visna (neurologically) affected sheep of the outbreak were used. Of the 350 clones obtained after PCR amplification, 142 corresponded to CNS samples (spinal cord and choroid plexus) and the remaining to mammary gland, blood cells, bronchoalveolar lavage cells and/or lung. The diversity of the <it>env </it>sequences from CNS was 11.1-16.1% between animals and 0.35-11.6% within each animal, except in one animal presenting two sequence types (30% diversity) in the CNS (one grouping with those of the outbreak), indicative of CNS virus sequence heterogeneity. Outbreak sequences were of genotype A, clustering per animal and compartmentalizing in the animal tissues. No CNS specific signature patterns were found.</p> <p>Conclusions</p> <p>Bayesian approach inferences suggested that proviruses from broncoalveolar lavage cells and peripheral blood mononuclear cells represented the common ancestors (infecting viruses) in the animal and that neuroinvasion in the outbreak involved microevolution after initial infection with an A-type strain. This study demonstrates virus compartmentalization in the CNS and other body tissues in sheep presenting the neurological form of SRLV infection.</p> |
| first_indexed | 2024-12-21T09:05:08Z |
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| id | doaj.art-8b581fa321aa41998611140c0ddd76e9 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-21T09:05:08Z |
| publishDate | 2012-01-01 |
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| series | BMC Veterinary Research |
| spelling | doaj.art-8b581fa321aa41998611140c0ddd76e92022-12-21T19:09:22ZengBMCBMC Veterinary Research1746-61482012-01-0181810.1186/1746-6148-8-8Study of compartmentalization in the visna clinical form of small ruminant lentivirus infection in sheepRamírez HugoReina RamsésBertolotti LuigiCenoz AmaiaHernández Mirna-MargaritaSan Román BeatrizGlaria Idoiade Andrés XimenaCrespo HelenaJáuregui PaulaBenavides JulioPolledo LauraPérez ValentínGarcía-Marín Juan FRosati SergioAmorena Beatrizde Andrés Damián<p>Abstract</p> <p>Background</p> <p>A central nervous system (CNS) disease outbreak caused by small ruminant lentiviruses (SRLV) has triggered interest in Spain due to the rapid onset of clinical signs and relevant production losses. In a previous study on this outbreak, the role of LTR in tropism was unclear and <it>env </it>encoded sequences, likely involved in tropism, were not investigated. This study aimed to analyze heterogeneity of SRLV Env regions - TM amino terminal and SU V4, C4 and V5 segments - in order to assess virus compartmentalization in CNS.</p> <p>Results</p> <p>Eight Visna (neurologically) affected sheep of the outbreak were used. Of the 350 clones obtained after PCR amplification, 142 corresponded to CNS samples (spinal cord and choroid plexus) and the remaining to mammary gland, blood cells, bronchoalveolar lavage cells and/or lung. The diversity of the <it>env </it>sequences from CNS was 11.1-16.1% between animals and 0.35-11.6% within each animal, except in one animal presenting two sequence types (30% diversity) in the CNS (one grouping with those of the outbreak), indicative of CNS virus sequence heterogeneity. Outbreak sequences were of genotype A, clustering per animal and compartmentalizing in the animal tissues. No CNS specific signature patterns were found.</p> <p>Conclusions</p> <p>Bayesian approach inferences suggested that proviruses from broncoalveolar lavage cells and peripheral blood mononuclear cells represented the common ancestors (infecting viruses) in the animal and that neuroinvasion in the outbreak involved microevolution after initial infection with an A-type strain. This study demonstrates virus compartmentalization in the CNS and other body tissues in sheep presenting the neurological form of SRLV infection.</p>http://www.biomedcentral.com/1746-6148/8/8CompartmentalizationVisnaSmall ruminant lentivirusSpinal cordChoroid plexusSheep |
| spellingShingle | Ramírez Hugo Reina Ramsés Bertolotti Luigi Cenoz Amaia Hernández Mirna-Margarita San Román Beatriz Glaria Idoia de Andrés Ximena Crespo Helena Jáuregui Paula Benavides Julio Polledo Laura Pérez Valentín García-Marín Juan F Rosati Sergio Amorena Beatriz de Andrés Damián Study of compartmentalization in the visna clinical form of small ruminant lentivirus infection in sheep BMC Veterinary Research Compartmentalization Visna Small ruminant lentivirus Spinal cord Choroid plexus Sheep |
| title | Study of compartmentalization in the visna clinical form of small ruminant lentivirus infection in sheep |
| title_full | Study of compartmentalization in the visna clinical form of small ruminant lentivirus infection in sheep |
| title_fullStr | Study of compartmentalization in the visna clinical form of small ruminant lentivirus infection in sheep |
| title_full_unstemmed | Study of compartmentalization in the visna clinical form of small ruminant lentivirus infection in sheep |
| title_short | Study of compartmentalization in the visna clinical form of small ruminant lentivirus infection in sheep |
| title_sort | study of compartmentalization in the visna clinical form of small ruminant lentivirus infection in sheep |
| topic | Compartmentalization Visna Small ruminant lentivirus Spinal cord Choroid plexus Sheep |
| url | http://www.biomedcentral.com/1746-6148/8/8 |
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