Establishment and identification of a medulloblastoma transplantable tumor strain PUMC-MBT1
Objective To establish a transplantable tumor strain of medulloblastoma in vivo from cell-line-derived xenograft (CDX) and to analyze the growth and pathological characteristics of the transplanted tumor model. Methods A previously established medulloblastoma cell line(PUMC-MB1) was utilized to cons...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-09-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-42-9-1339.pdf |
| _version_ | 1797365867923636224 |
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| author | WANG Shi-zun, ZHANG Dan, GE Ming, FENG Hai-liang, LIU Yu-qin |
| author_facet | WANG Shi-zun, ZHANG Dan, GE Ming, FENG Hai-liang, LIU Yu-qin |
| author_sort | WANG Shi-zun, ZHANG Dan, GE Ming, FENG Hai-liang, LIU Yu-qin |
| collection | DOAJ |
| description | Objective To establish a transplantable tumor strain of medulloblastoma in vivo from cell-line-derived xenograft (CDX) and to analyze the growth and pathological characteristics of the transplanted tumor model. Methods A previously established medulloblastoma cell line(PUMC-MB1) was utilized to construct CDX. The cultured cell suspension was injected into the right axilla of SCID mice subcutaneously with dosage as 5×106 cells/mouse. The mice were sacrificed when the diameter of CDX reached around 1.5 cm. The xenografts were harvested and transplanted to other mice with the same number of cells. The process was repeated 10 times. The growth characteristics of transplanted medulloblastoma and survival curve of tumor-bearing mice at passage 10 were observed. The pathological characteristics of transplanted tumor were analyzed. Results The transplantable strain of medullo- blastoma with stable in vivo properties was named PUMC-MBT1. The successful rate of tumor transplantation was 100% at passage 10. The subcutaneous mass could be palpated 14 days after injection. The diameters of subcutaneous xenograft could reach about 1.5 cm 29 days after injection. Tumor-bearing mice died 50 days after injection. HE sections demonstrated that the transplanted PUMC-MBT1 belonged to classic medulloblastoma and the positive rate of Ki-67, Syn and NeuN were 80%-90%, 40%-50% and 20%-30% respectively. Conclusions A successful animal model of medulloblastoma is established with easy manipulation, characteristic features of classic medulloblastoma, so it is a potential technology for in vivo research. |
| first_indexed | 2024-03-08T16:55:05Z |
| format | Article |
| id | doaj.art-8b58215547a7492eaeca0f84df310b50 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:55:05Z |
| publishDate | 2022-09-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-8b58215547a7492eaeca0f84df310b502024-01-05T02:36:18ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-09-014291339134310.16352/j.issn.1001-6325.2022.09.1339Establishment and identification of a medulloblastoma transplantable tumor strain PUMC-MBT1WANG Shi-zun, ZHANG Dan, GE Ming, FENG Hai-liang, LIU Yu-qin01. Department of Pathology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005;;2. Cell Resource Center, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005;;3. Department of Neurosurgery, Beijing Children Hospital, Capital Medical University, National Center for Children’s Health, Beijing 100045, ChinaObjective To establish a transplantable tumor strain of medulloblastoma in vivo from cell-line-derived xenograft (CDX) and to analyze the growth and pathological characteristics of the transplanted tumor model. Methods A previously established medulloblastoma cell line(PUMC-MB1) was utilized to construct CDX. The cultured cell suspension was injected into the right axilla of SCID mice subcutaneously with dosage as 5×106 cells/mouse. The mice were sacrificed when the diameter of CDX reached around 1.5 cm. The xenografts were harvested and transplanted to other mice with the same number of cells. The process was repeated 10 times. The growth characteristics of transplanted medulloblastoma and survival curve of tumor-bearing mice at passage 10 were observed. The pathological characteristics of transplanted tumor were analyzed. Results The transplantable strain of medullo- blastoma with stable in vivo properties was named PUMC-MBT1. The successful rate of tumor transplantation was 100% at passage 10. The subcutaneous mass could be palpated 14 days after injection. The diameters of subcutaneous xenograft could reach about 1.5 cm 29 days after injection. Tumor-bearing mice died 50 days after injection. HE sections demonstrated that the transplanted PUMC-MBT1 belonged to classic medulloblastoma and the positive rate of Ki-67, Syn and NeuN were 80%-90%, 40%-50% and 20%-30% respectively. Conclusions A successful animal model of medulloblastoma is established with easy manipulation, characteristic features of classic medulloblastoma, so it is a potential technology for in vivo research.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-42-9-1339.pdfmedulloblastoma| cell-line-derived xenograft (cdx)| tumor strain |
| spellingShingle | WANG Shi-zun, ZHANG Dan, GE Ming, FENG Hai-liang, LIU Yu-qin Establishment and identification of a medulloblastoma transplantable tumor strain PUMC-MBT1 Jichu yixue yu linchuang medulloblastoma| cell-line-derived xenograft (cdx)| tumor strain |
| title | Establishment and identification of a medulloblastoma transplantable tumor strain PUMC-MBT1 |
| title_full | Establishment and identification of a medulloblastoma transplantable tumor strain PUMC-MBT1 |
| title_fullStr | Establishment and identification of a medulloblastoma transplantable tumor strain PUMC-MBT1 |
| title_full_unstemmed | Establishment and identification of a medulloblastoma transplantable tumor strain PUMC-MBT1 |
| title_short | Establishment and identification of a medulloblastoma transplantable tumor strain PUMC-MBT1 |
| title_sort | establishment and identification of a medulloblastoma transplantable tumor strain pumc mbt1 |
| topic | medulloblastoma| cell-line-derived xenograft (cdx)| tumor strain |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-42-9-1339.pdf |
| work_keys_str_mv | AT wangshizunzhangdangemingfenghailiangliuyuqin establishmentandidentificationofamedulloblastomatransplantabletumorstrainpumcmbt1 |