Activated producing HOCL neutrophils revealed by flow cytometry and confocal microscopy with celestine blue B

Activated producing HOCL neutrophils revealed by flow cytometry and confocal microscopy with celestine blue B

Hypochlorous acid (HOCl) is produced with myeloperoxidase (MPO) in activated neutrophils. To assay MPO activity we chose a HOCl-selective dye i.e. celestine blue B (CB) that changes into pink glycol after oxidation. Our aim was to elaborate fluorescent methods of measuring HOCl production with activ...

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Bibliographic Details
Main Authors:	S. O. Kozlov, I. V. Kudryavtsev, N. A. Grudinina, V. A. Kostevich, O. M. Panasenko, A. V. Sokolov, V. B. Vasilyev
Format:	Article
Language:	Russian
Published:	Scientific Сentre for Family Health and Human Reproduction Problems 2016-05-01
Series:	Acta Biomedica Scientifica
Subjects:	hocl hypochlorous acid myeloperoxidase celestine blue b neutrophil confocal microscopy flow cytometry fluorescence
Online Access:	https://www.actabiomedica.ru/jour/article/view/194

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