The Effect of Food Deprivation on Nociception in Formalin Test and Plasma Levels of Noradrenaline and Corticosterone in Rats
Introduction: The concentration of noradrenalin and corticosterone as the two nociception modulators change after fasting or stress situation. The aim of present study was to investigate the effect of food deprivation on formalin-induced nociceptive behaviours and plasma levels of noradrenalin and c...
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| Format: | Article |
| Language: | English |
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Iran University of Medical Sciences
2013-11-01
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| Series: | Basic and Clinical Neuroscience |
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| Online Access: | http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-184&slc_lang=en&sid=1 |
| _version_ | 1797282771646808064 |
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| author | Nematollah Gheibi Mohammadreza Saroukhani Hassan Azhdari-Zarmehri |
| author_facet | Nematollah Gheibi Mohammadreza Saroukhani Hassan Azhdari-Zarmehri |
| author_sort | Nematollah Gheibi |
| collection | DOAJ |
| description | Introduction: The concentration of noradrenalin and corticosterone as the two nociception modulators change after fasting or stress situation. The aim of present study was to investigate the effect of food deprivation on formalin-induced nociceptive behaviours and plasma levels of noradrenalin and corticosterone in rats. Methods: Food was withdrawn 12, 24 and 48 h prior to performing the formalin test, but water continued to be available ad libitum. The formalin solution (50 μL, 2%) was injected into plantar surface of hind paw. The nociception responses of the animals during the first phase (1-7 minutes), the inter-phase (8-14), the phase 2A (15-60) and the phase 2B (61-90) was separately evaluated. The plasma concentrations of noradrenalin and corticosterone were measured using specific ELISA and IRA kits, according to manufacturer's instructions. Results: In contrast to the increasing of 48 h food deprived animals during phase 2, the nociceptive behaviours of 12 and 24 h groups decreased through the interphase, phase 2A and phase 2B. The injection of formalin in the normal male rats significantly decreased the plasma level of noradrenalin and corticosterone. Food deprivation for 12 and 24 h increased noradrenalin level significantly in comparison with control group which has caused by fasting induced antinociceptive behaviours. There was no significant change in food deprivation for 48 h group. Food deprivation for 12, 24 and 48 h had no effect on corticosterone level in male rats. Discussion: The present study emphasizes that the acute food deprivation diminished the nociceptive behaviours in the formalin test and show a correlation with increase in plasma noradrenalin level. |
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| format | Article |
| id | doaj.art-8b61dc1db4db417cac92093e863ab7b4 |
| institution | Directory Open Access Journal |
| issn | 2008-126X 2228-7442 |
| language | English |
| last_indexed | 2024-03-07T17:18:15Z |
| publishDate | 2013-11-01 |
| publisher | Iran University of Medical Sciences |
| record_format | Article |
| series | Basic and Clinical Neuroscience |
| spelling | doaj.art-8b61dc1db4db417cac92093e863ab7b42024-03-02T21:26:40ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422013-11-0144341347The Effect of Food Deprivation on Nociception in Formalin Test and Plasma Levels of Noradrenaline and Corticosterone in RatsNematollah Gheibi0Mohammadreza Saroukhani1Hassan Azhdari-Zarmehri2 Introduction: The concentration of noradrenalin and corticosterone as the two nociception modulators change after fasting or stress situation. The aim of present study was to investigate the effect of food deprivation on formalin-induced nociceptive behaviours and plasma levels of noradrenalin and corticosterone in rats. Methods: Food was withdrawn 12, 24 and 48 h prior to performing the formalin test, but water continued to be available ad libitum. The formalin solution (50 μL, 2%) was injected into plantar surface of hind paw. The nociception responses of the animals during the first phase (1-7 minutes), the inter-phase (8-14), the phase 2A (15-60) and the phase 2B (61-90) was separately evaluated. The plasma concentrations of noradrenalin and corticosterone were measured using specific ELISA and IRA kits, according to manufacturer's instructions. Results: In contrast to the increasing of 48 h food deprived animals during phase 2, the nociceptive behaviours of 12 and 24 h groups decreased through the interphase, phase 2A and phase 2B. The injection of formalin in the normal male rats significantly decreased the plasma level of noradrenalin and corticosterone. Food deprivation for 12 and 24 h increased noradrenalin level significantly in comparison with control group which has caused by fasting induced antinociceptive behaviours. There was no significant change in food deprivation for 48 h group. Food deprivation for 12, 24 and 48 h had no effect on corticosterone level in male rats. Discussion: The present study emphasizes that the acute food deprivation diminished the nociceptive behaviours in the formalin test and show a correlation with increase in plasma noradrenalin level.http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-184&slc_lang=en&sid=1RatFood DeprivationNoradrenalinCorticosteroneFormalin Test. |
| spellingShingle | Nematollah Gheibi Mohammadreza Saroukhani Hassan Azhdari-Zarmehri The Effect of Food Deprivation on Nociception in Formalin Test and Plasma Levels of Noradrenaline and Corticosterone in Rats Basic and Clinical Neuroscience Rat Food Deprivation Noradrenalin Corticosterone Formalin Test. |
| title | The Effect of Food Deprivation on Nociception in Formalin Test and Plasma Levels of Noradrenaline and Corticosterone in Rats |
| title_full | The Effect of Food Deprivation on Nociception in Formalin Test and Plasma Levels of Noradrenaline and Corticosterone in Rats |
| title_fullStr | The Effect of Food Deprivation on Nociception in Formalin Test and Plasma Levels of Noradrenaline and Corticosterone in Rats |
| title_full_unstemmed | The Effect of Food Deprivation on Nociception in Formalin Test and Plasma Levels of Noradrenaline and Corticosterone in Rats |
| title_short | The Effect of Food Deprivation on Nociception in Formalin Test and Plasma Levels of Noradrenaline and Corticosterone in Rats |
| title_sort | effect of food deprivation on nociception in formalin test and plasma levels of noradrenaline and corticosterone in rats |
| topic | Rat Food Deprivation Noradrenalin Corticosterone Formalin Test. |
| url | http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-184&slc_lang=en&sid=1 |
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