Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology
Permethrin (PERM) is a member of the class I family of synthetic pyrethroids. Human use has shown that it affects different systems, with wide health dysfunctions. Our aim was to determine bioenergetics, neuroinflammation and morphology changes, as redox markers after subacute exposure to PERM in ra...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | Toxics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2305-6304/9/12/337 |
| _version_ | 1797500242914967552 |
|---|---|
| author | Teresita Guadalupe López-Aceves Elvia Coballase-Urrutia Francisco Estrada-Rojo América Vanoye-Carlo Liliana Carmona-Aparicio María Eugenia Hernández José Pedraza-Chaverri Luz Navarro Omar E. Aparicio-Trejo Armando Pérez-Torres Omar N. Medina-Campos Daniel Martínez-Fong Vicente Sánchez-Valle Noemi Cárdenas-Rodríguez Leticia Granados-Rojas Evelyn Pulido-Camarillo Verónica Rodríguez-Mata Claudia del R. León-Sicairos |
| author_facet | Teresita Guadalupe López-Aceves Elvia Coballase-Urrutia Francisco Estrada-Rojo América Vanoye-Carlo Liliana Carmona-Aparicio María Eugenia Hernández José Pedraza-Chaverri Luz Navarro Omar E. Aparicio-Trejo Armando Pérez-Torres Omar N. Medina-Campos Daniel Martínez-Fong Vicente Sánchez-Valle Noemi Cárdenas-Rodríguez Leticia Granados-Rojas Evelyn Pulido-Camarillo Verónica Rodríguez-Mata Claudia del R. León-Sicairos |
| author_sort | Teresita Guadalupe López-Aceves |
| collection | DOAJ |
| description | Permethrin (PERM) is a member of the class I family of synthetic pyrethroids. Human use has shown that it affects different systems, with wide health dysfunctions. Our aim was to determine bioenergetics, neuroinflammation and morphology changes, as redox markers after subacute exposure to PERM in rats. We used MDA determination, protein carbonyl assay, mitochondrial O<sub>2</sub> consumption, expression of pro-inflammatory cytokines and a deep histopathological analysis of the hippocampus. PERM (150 mg/kg and 300 mg/kg body weight/day, o.v.) increased lipoperoxidation and carbonylated proteins in a dose-dependent manner in the brain regions. The activities of antioxidant enzymes glutathione peroxidase, reductase, S-transferase, catalase, and superoxide dismutase showed an increase in all the different brain areas, with dose-dependent effects in the cerebellum. Cytokine profiles (IL-1β, IL-6 and TNF-α) increased in a dose-dependent manner in different brain tissues. Exposure to 150 mg/kg of permethrin induced degenerated and/or dead neurons in the rat hippocampus and induced mitochondrial uncoupling and reduction of oxidative phosphorylation and significantly decreased the respiratory parameters state 3-associated respiration in complex I and II. PERM exposure at low doses induces reactive oxygen species production and imbalance in the enzymatic antioxidant system, increases gene expression of pro-inflammatory interleukins, and could lead to cell damage mediated by mitochondrial functional impairment. |
| first_indexed | 2024-03-10T03:59:07Z |
| format | Article |
| id | doaj.art-8b624127c82541e0b1d73f250375d8bd |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-10T03:59:07Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-8b624127c82541e0b1d73f250375d8bd2023-11-23T10:49:49ZengMDPI AGToxics2305-63042021-12-0191233710.3390/toxics9120337Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and MorphologyTeresita Guadalupe López-Aceves0Elvia Coballase-Urrutia1Francisco Estrada-Rojo2América Vanoye-Carlo3Liliana Carmona-Aparicio4María Eugenia Hernández5José Pedraza-Chaverri6Luz Navarro7Omar E. Aparicio-Trejo8Armando Pérez-Torres9Omar N. Medina-Campos10Daniel Martínez-Fong11Vicente Sánchez-Valle12Noemi Cárdenas-Rodríguez13Leticia Granados-Rojas14Evelyn Pulido-Camarillo15Verónica Rodríguez-Mata16Claudia del R. León-Sicairos17Regional Graduate Program in Biotechnology, Faculty of Biological Chemical Sciences, Autonomous University of Sinaloa, Culiacán 80000, MexicoLaboratory of Neuroscience, National Institute of Pediatrics, Mexico City 04530, MexicoDepartment of Physiology, Faculty of Medicine, National Autonomous University of Mexico, Mexico City 04510, MexicoLaboratory of Neuroscience, National Institute of Pediatrics, Mexico City 04530, MexicoLaboratory of Neuroscience, National Institute of Pediatrics, Mexico City 04530, MexicoSubdirection of Clinical Research, National Institute of Psychiatry, Mexico City 14370, MexicoDepartment of Biology, Faculty of Chemistry, National Autonomous University of Mexico, Mexico City 04150, MexicoDepartment of Physiology, Faculty of Medicine, National Autonomous University of Mexico, Mexico City 04510, MexicoDepartment of Biology, Faculty of Chemistry, National Autonomous University of Mexico, Mexico City 04150, MexicoDepartment of Cell and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Mexico City 04510, MexicoDepartment of Biology, Faculty of Chemistry, National Autonomous University of Mexico, Mexico City 04150, MexicoDepartment of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies, Mexico City 07360, MexicoNeuroplasticity and Neurodegeneration Laboratory, Department of Pharmacology, Center for Research and Advanced Studies, Mexico City 07360, MexicoLaboratory of Neuroscience, National Institute of Pediatrics, Mexico City 04530, MexicoLaboratory of Neuroscience, National Institute of Pediatrics, Mexico City 04530, MexicoDepartment of Cell and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Mexico City 04510, MexicoDepartment of Cell and Tissue Biology, Faculty of Medicine, National Autonomous University of Mexico, Mexico City 04510, MexicoRegional Graduate Program in Biotechnology, Faculty of Biological Chemical Sciences, Autonomous University of Sinaloa, Culiacán 80000, MexicoPermethrin (PERM) is a member of the class I family of synthetic pyrethroids. Human use has shown that it affects different systems, with wide health dysfunctions. Our aim was to determine bioenergetics, neuroinflammation and morphology changes, as redox markers after subacute exposure to PERM in rats. We used MDA determination, protein carbonyl assay, mitochondrial O<sub>2</sub> consumption, expression of pro-inflammatory cytokines and a deep histopathological analysis of the hippocampus. PERM (150 mg/kg and 300 mg/kg body weight/day, o.v.) increased lipoperoxidation and carbonylated proteins in a dose-dependent manner in the brain regions. The activities of antioxidant enzymes glutathione peroxidase, reductase, S-transferase, catalase, and superoxide dismutase showed an increase in all the different brain areas, with dose-dependent effects in the cerebellum. Cytokine profiles (IL-1β, IL-6 and TNF-α) increased in a dose-dependent manner in different brain tissues. Exposure to 150 mg/kg of permethrin induced degenerated and/or dead neurons in the rat hippocampus and induced mitochondrial uncoupling and reduction of oxidative phosphorylation and significantly decreased the respiratory parameters state 3-associated respiration in complex I and II. PERM exposure at low doses induces reactive oxygen species production and imbalance in the enzymatic antioxidant system, increases gene expression of pro-inflammatory interleukins, and could lead to cell damage mediated by mitochondrial functional impairment.https://www.mdpi.com/2305-6304/9/12/337permethrinneurotoxicitybioenergeticsneuroinflammation |
| spellingShingle | Teresita Guadalupe López-Aceves Elvia Coballase-Urrutia Francisco Estrada-Rojo América Vanoye-Carlo Liliana Carmona-Aparicio María Eugenia Hernández José Pedraza-Chaverri Luz Navarro Omar E. Aparicio-Trejo Armando Pérez-Torres Omar N. Medina-Campos Daniel Martínez-Fong Vicente Sánchez-Valle Noemi Cárdenas-Rodríguez Leticia Granados-Rojas Evelyn Pulido-Camarillo Verónica Rodríguez-Mata Claudia del R. León-Sicairos Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology Toxics permethrin neurotoxicity bioenergetics neuroinflammation |
| title | Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology |
| title_full | Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology |
| title_fullStr | Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology |
| title_full_unstemmed | Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology |
| title_short | Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology |
| title_sort | exposure to sub lethal doses of permethrin is associated with neurotoxicity changes in bioenergetics redox markers neuroinflammation and morphology |
| topic | permethrin neurotoxicity bioenergetics neuroinflammation |
| url | https://www.mdpi.com/2305-6304/9/12/337 |
| work_keys_str_mv | AT teresitaguadalupelopezaceves exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT elviacoballaseurrutia exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT franciscoestradarojo exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT americavanoyecarlo exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT lilianacarmonaaparicio exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT mariaeugeniahernandez exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT josepedrazachaverri exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT luznavarro exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT omareapariciotrejo exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT armandopereztorres exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT omarnmedinacampos exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT danielmartinezfong exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT vicentesanchezvalle exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT noemicardenasrodriguez exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT leticiagranadosrojas exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT evelynpulidocamarillo exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT veronicarodriguezmata exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology AT claudiadelrleonsicairos exposuretosublethaldosesofpermethrinisassociatedwithneurotoxicitychangesinbioenergeticsredoxmarkersneuroinflammationandmorphology |