Biomarker Discovery for Detecting the Seed Ageing Degree and Priming Effect of Tobacco
Seed ageing is an important issue for the long-time seed storage of tobacco. Seed priming has been popularly applied in tobacco production. In this study, the development of molecular marker genes encoding proteins L-isoaspartyl methyltransferase NtPIMT1 and 8-oxoG DNA glycosylase 1 NtOGG1 to detect...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
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| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/12/8/1897 |
| Summary: | Seed ageing is an important issue for the long-time seed storage of tobacco. Seed priming has been popularly applied in tobacco production. In this study, the development of molecular marker genes encoding proteins L-isoaspartyl methyltransferase NtPIMT1 and 8-oxoG DNA glycosylase 1 NtOGG1 to detect the degree of seed ageing and the effect of seed priming is conducted in tobacco. Quantitative real-time PCR (qRT-PCR) analysis reveals that relatively higher mRNAs of <i>NtPIMT1</i> and <i>NtOGG1</i> are observed in the dry and early germinating seeds. The expressions of <i>NtPIMT1</i> and <i>NtOGG1</i> are negatively correlated with the degree of seed damage in non-pelleted and pelleted seeds after accelerated ageing treatments. The early best effects of gibberellin (GA<sub>3</sub>) priming on speed and uniform germination are observed in 33 h primed seeds, and relatively lower expressions of <i>NtPIMT1</i> and <i>NtOGG1</i> are observed in priming seeds. <i>NtPIMT1</i> and <i>NtOGG1</i> genes have potential for use as molecular markers in detecting the seed ageing degree and priming effect of tobacco. |
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| ISSN: | 2073-4395 |