lncRNA-AC079061.1/VIPR1 axis may suppress the development of hepatocellular carcinoma: a bioinformatics analysis and experimental validation
Abstract Background Hepatocellular carcinoma (HCC) is one of the most malignant tumors to threaten human life, and the survival rate remains low due to delayed diagnosis. Meanwhile, lncRNAs have great potential for application in tumor prognosis, therefore relevant research in hepatocellular carcino...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12967-022-03573-7 |
| _version_ | 1817994131239075840 |
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| author | Xia-Hui Lin Dan-Ying Zhang Zhi-Yong Liu Wen-qing Tang Rong-Xin Chen Dong-ping Li Shuqiang Weng Ling Dong |
| author_facet | Xia-Hui Lin Dan-Ying Zhang Zhi-Yong Liu Wen-qing Tang Rong-Xin Chen Dong-ping Li Shuqiang Weng Ling Dong |
| author_sort | Xia-Hui Lin |
| collection | DOAJ |
| description | Abstract Background Hepatocellular carcinoma (HCC) is one of the most malignant tumors to threaten human life, and the survival rate remains low due to delayed diagnosis. Meanwhile, lncRNAs have great potential for application in tumor prognosis, therefore relevant research in hepatocellular carcinoma is indispensable. Methods Based on the EZH2 expression, the differentially expressed lncRNAs DElncRNAs), miRNAs (DEmiRNAs), and mRNAs (DEmRNAs) were identified in hepatocellular carcinoma by using the TCGA database. Bioinformatics technology was utilized to determine the effect of key genes in HCC progression. The methylation and immune infiltration analyses were performed to explore the underlying function of hub genes. Finally, cellular function experiments were performed to investigate the association between identified genes and biological phenotypes in HCC. Results lncRNA-AC079061.1, hsa-miR-765, and VIPR1 were identified as independent factors that affect the prognosis of hepatocellular carcinoma. The immune infiltration analyses revealed that lncRNA-AC079061.1 can alter the immune microenvironment and thus inhibit the development of HCC by regulating the expression of an immune-related gene (VIPR1). Methylation analyses demonstrated that VIPR1 expression is negatively related to the methylation level in HCC. Experimental results suggested that lncRNA-AC079061.1 and VIPR1 were frequently downregulated in HCC cells, while hsa-miR-765 was significantly upregulated. Moreover, the lncRNA-AC079061.1/VIPR1 axis suppressed the proliferation and invasion of HCC cells. Conclusion The present study identified the lncRNA-AC079061.1/VIPR1 axis as a novel biomarker that inhibited the proliferation and invasion of hepatocellular carcinoma, affecting the ultimate disease outcome. |
| first_indexed | 2024-04-14T01:47:48Z |
| format | Article |
| id | doaj.art-8b64c4a6816443fcae9304f11125709a |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-04-14T01:47:48Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-8b64c4a6816443fcae9304f11125709a2022-12-22T02:19:27ZengBMCJournal of Translational Medicine1479-58762022-08-0120112110.1186/s12967-022-03573-7lncRNA-AC079061.1/VIPR1 axis may suppress the development of hepatocellular carcinoma: a bioinformatics analysis and experimental validationXia-Hui Lin0Dan-Ying Zhang1Zhi-Yong Liu2Wen-qing Tang3Rong-Xin Chen4Dong-ping Li5Shuqiang Weng6Ling Dong7Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan UniversityDepartment of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan UniversityDepartment of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan UniversityDepartment of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan UniversityLiver Cancer Institute, Zhongshan Hospital, Fudan University and Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of EducationDepartment of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan UniversityDepartment of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan UniversityDepartment of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan UniversityAbstract Background Hepatocellular carcinoma (HCC) is one of the most malignant tumors to threaten human life, and the survival rate remains low due to delayed diagnosis. Meanwhile, lncRNAs have great potential for application in tumor prognosis, therefore relevant research in hepatocellular carcinoma is indispensable. Methods Based on the EZH2 expression, the differentially expressed lncRNAs DElncRNAs), miRNAs (DEmiRNAs), and mRNAs (DEmRNAs) were identified in hepatocellular carcinoma by using the TCGA database. Bioinformatics technology was utilized to determine the effect of key genes in HCC progression. The methylation and immune infiltration analyses were performed to explore the underlying function of hub genes. Finally, cellular function experiments were performed to investigate the association between identified genes and biological phenotypes in HCC. Results lncRNA-AC079061.1, hsa-miR-765, and VIPR1 were identified as independent factors that affect the prognosis of hepatocellular carcinoma. The immune infiltration analyses revealed that lncRNA-AC079061.1 can alter the immune microenvironment and thus inhibit the development of HCC by regulating the expression of an immune-related gene (VIPR1). Methylation analyses demonstrated that VIPR1 expression is negatively related to the methylation level in HCC. Experimental results suggested that lncRNA-AC079061.1 and VIPR1 were frequently downregulated in HCC cells, while hsa-miR-765 was significantly upregulated. Moreover, the lncRNA-AC079061.1/VIPR1 axis suppressed the proliferation and invasion of HCC cells. Conclusion The present study identified the lncRNA-AC079061.1/VIPR1 axis as a novel biomarker that inhibited the proliferation and invasion of hepatocellular carcinoma, affecting the ultimate disease outcome.https://doi.org/10.1186/s12967-022-03573-7Hepatocellular carcinoma (HCC)ceRNA mechanismBioinformatics analysisProliferationInvasionPrognosis |
| spellingShingle | Xia-Hui Lin Dan-Ying Zhang Zhi-Yong Liu Wen-qing Tang Rong-Xin Chen Dong-ping Li Shuqiang Weng Ling Dong lncRNA-AC079061.1/VIPR1 axis may suppress the development of hepatocellular carcinoma: a bioinformatics analysis and experimental validation Journal of Translational Medicine Hepatocellular carcinoma (HCC) ceRNA mechanism Bioinformatics analysis Proliferation Invasion Prognosis |
| title | lncRNA-AC079061.1/VIPR1 axis may suppress the development of hepatocellular carcinoma: a bioinformatics analysis and experimental validation |
| title_full | lncRNA-AC079061.1/VIPR1 axis may suppress the development of hepatocellular carcinoma: a bioinformatics analysis and experimental validation |
| title_fullStr | lncRNA-AC079061.1/VIPR1 axis may suppress the development of hepatocellular carcinoma: a bioinformatics analysis and experimental validation |
| title_full_unstemmed | lncRNA-AC079061.1/VIPR1 axis may suppress the development of hepatocellular carcinoma: a bioinformatics analysis and experimental validation |
| title_short | lncRNA-AC079061.1/VIPR1 axis may suppress the development of hepatocellular carcinoma: a bioinformatics analysis and experimental validation |
| title_sort | lncrna ac079061 1 vipr1 axis may suppress the development of hepatocellular carcinoma a bioinformatics analysis and experimental validation |
| topic | Hepatocellular carcinoma (HCC) ceRNA mechanism Bioinformatics analysis Proliferation Invasion Prognosis |
| url | https://doi.org/10.1186/s12967-022-03573-7 |
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