Rat hepatitis E virus (Rocahepevirus ratti) exposure in cats and dogs, Hong Kong
ABSTRACTHepatitis E virus (HEV) variants infecting humans belong to two species: Paslahepevirus balayani (bHEV) and Rocahepevirus ratti (rat hepatitis E virus; rHEV). R. ratti is a ubiquitous rodent pathogen that has recently been recognized to cause hepatitis in humans. Transmission routes of rHEV...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2024.2337671 |
| _version_ | 1797213059553427456 |
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| author | Estie Hon-Kiu Shun Jianwen Situ James Yiu-Hung Tsoi Shusheng Wu Jianpiao Cai Kelvin Hon-Yin Lo Nicholas Foo-Siong Chew Zhiyu Li Rosana Wing-Shan Poon Jade Lee-Lee Teng Vincent Chi-Chung Cheng Kwok-Yung Yuen Siddharth Sridhar |
| author_facet | Estie Hon-Kiu Shun Jianwen Situ James Yiu-Hung Tsoi Shusheng Wu Jianpiao Cai Kelvin Hon-Yin Lo Nicholas Foo-Siong Chew Zhiyu Li Rosana Wing-Shan Poon Jade Lee-Lee Teng Vincent Chi-Chung Cheng Kwok-Yung Yuen Siddharth Sridhar |
| author_sort | Estie Hon-Kiu Shun |
| collection | DOAJ |
| description | ABSTRACTHepatitis E virus (HEV) variants infecting humans belong to two species: Paslahepevirus balayani (bHEV) and Rocahepevirus ratti (rat hepatitis E virus; rHEV). R. ratti is a ubiquitous rodent pathogen that has recently been recognized to cause hepatitis in humans. Transmission routes of rHEV from rats to humans are currently unknown. In this study, we examined rHEV exposure in cats and dogs to determine if they are potential reservoirs of this emerging human pathogen. Virus-like particle-based IgG enzymatic immunoassays (EIAs) capable of differentiating rHEV & bHEV antibody profiles and rHEV-specific real-time RT–PCR assays were used for this purpose. The EIAs could detect bHEV and rHEV patient-derived IgG spiked in dog and cat sera. Sera from 751 companion dogs and 130 companion cats in Hong Kong were tested with these IgG enzymatic immunoassays (EIAs). Overall, 13/751 (1.7%) dogs and 5/130 (3.8%) cats were sero-reactive to HEV. 9/751 (1.2%) dogs and 2/130 (1.5%) cats tested positive for rHEV IgG, which was further confirmed by rHEV immunoblots. Most rHEV-seropositive animals were from areas in or adjacent to districts reporting human rHEV infection. Neither 881 companion animals nor 652 stray animals carried rHEV RNA in serum or rectal swabs. Therefore, we could not confirm a role for cats and dogs in transmitting rHEV to humans. Further work is required to understand the reasons for low-level seropositivity in these animals. |
| first_indexed | 2024-04-24T10:52:15Z |
| format | Article |
| id | doaj.art-8b66066f4054427d888bfd91e591ce90 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-04-24T10:52:15Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-8b66066f4054427d888bfd91e591ce902024-04-12T09:25:09ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512024-12-0113110.1080/22221751.2024.2337671Rat hepatitis E virus (Rocahepevirus ratti) exposure in cats and dogs, Hong KongEstie Hon-Kiu Shun0Jianwen Situ1James Yiu-Hung Tsoi2Shusheng Wu3Jianpiao Cai4Kelvin Hon-Yin Lo5Nicholas Foo-Siong Chew6Zhiyu Li7Rosana Wing-Shan Poon8Jade Lee-Lee Teng9Vincent Chi-Chung Cheng10Kwok-Yung Yuen11Siddharth Sridhar12Department of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, People’s Republic of ChinaFaculty of Dentistry, The University of Hong Kong, Hong Kong, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, People’s Republic of ChinaDepartment of Microbiology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, People’s Republic of ChinaABSTRACTHepatitis E virus (HEV) variants infecting humans belong to two species: Paslahepevirus balayani (bHEV) and Rocahepevirus ratti (rat hepatitis E virus; rHEV). R. ratti is a ubiquitous rodent pathogen that has recently been recognized to cause hepatitis in humans. Transmission routes of rHEV from rats to humans are currently unknown. In this study, we examined rHEV exposure in cats and dogs to determine if they are potential reservoirs of this emerging human pathogen. Virus-like particle-based IgG enzymatic immunoassays (EIAs) capable of differentiating rHEV & bHEV antibody profiles and rHEV-specific real-time RT–PCR assays were used for this purpose. The EIAs could detect bHEV and rHEV patient-derived IgG spiked in dog and cat sera. Sera from 751 companion dogs and 130 companion cats in Hong Kong were tested with these IgG enzymatic immunoassays (EIAs). Overall, 13/751 (1.7%) dogs and 5/130 (3.8%) cats were sero-reactive to HEV. 9/751 (1.2%) dogs and 2/130 (1.5%) cats tested positive for rHEV IgG, which was further confirmed by rHEV immunoblots. Most rHEV-seropositive animals were from areas in or adjacent to districts reporting human rHEV infection. Neither 881 companion animals nor 652 stray animals carried rHEV RNA in serum or rectal swabs. Therefore, we could not confirm a role for cats and dogs in transmitting rHEV to humans. Further work is required to understand the reasons for low-level seropositivity in these animals.https://www.tandfonline.com/doi/10.1080/22221751.2024.2337671Hepatitis E virushepatitis antibodiesratscat diseasesdogs |
| spellingShingle | Estie Hon-Kiu Shun Jianwen Situ James Yiu-Hung Tsoi Shusheng Wu Jianpiao Cai Kelvin Hon-Yin Lo Nicholas Foo-Siong Chew Zhiyu Li Rosana Wing-Shan Poon Jade Lee-Lee Teng Vincent Chi-Chung Cheng Kwok-Yung Yuen Siddharth Sridhar Rat hepatitis E virus (Rocahepevirus ratti) exposure in cats and dogs, Hong Kong Emerging Microbes and Infections Hepatitis E virus hepatitis antibodies rats cat diseases dogs |
| title | Rat hepatitis E virus (Rocahepevirus ratti) exposure in cats and dogs, Hong Kong |
| title_full | Rat hepatitis E virus (Rocahepevirus ratti) exposure in cats and dogs, Hong Kong |
| title_fullStr | Rat hepatitis E virus (Rocahepevirus ratti) exposure in cats and dogs, Hong Kong |
| title_full_unstemmed | Rat hepatitis E virus (Rocahepevirus ratti) exposure in cats and dogs, Hong Kong |
| title_short | Rat hepatitis E virus (Rocahepevirus ratti) exposure in cats and dogs, Hong Kong |
| title_sort | rat hepatitis e virus rocahepevirus ratti exposure in cats and dogs hong kong |
| topic | Hepatitis E virus hepatitis antibodies rats cat diseases dogs |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2024.2337671 |
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