Identification of proteins associated with changes in oligosaccharide metabolism in Lycium barbarum L. fruit by using iTRAQ technology
Oligosaccharides are important functional active components of Lycium barbarum L. (LBL, “Goji”) fruits, but their metabolic mechanisms are not yet clear. In this study, we firstly investigated the changes in the content of oligosaccharides in LBL at different stages (S1–S5), and found that it mainly...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Journal of Agriculture and Food Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154323001199 |
| _version_ | 1797823094296936448 |
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| author | Pengyan Gao Peipei Li Zefu Li Jun Liu Anran Zheng Ziyin Hu Runyu Wang Dunhua Liu |
| author_facet | Pengyan Gao Peipei Li Zefu Li Jun Liu Anran Zheng Ziyin Hu Runyu Wang Dunhua Liu |
| author_sort | Pengyan Gao |
| collection | DOAJ |
| description | Oligosaccharides are important functional active components of Lycium barbarum L. (LBL, “Goji”) fruits, but their metabolic mechanisms are not yet clear. In this study, we firstly investigated the changes in the content of oligosaccharides in LBL at different stages (S1–S5), and found that it mainly accumulated at S3 and S4. Secondly, the structural characteristics of LBL oligosaccharide (LBO) were studied by GC-MS and FT-IR. Thirdly, using iTRAQ proteomics analysis, we identified 135 differentially expressed proteins associated with oligosaccharide metabolism, which is mainly accomplished through the collaboration of a range of key enzymes. At S1 and S2, oligosaccharide synthesis was achieved mainly through photosynthesis, and changes in anabolism were mainly associated with the expression of sucrose synthase, sucrose phosphate synthase and hexokinase. Oligosaccharide accumulation was achieved through starch and polysaccharide metabolism and cellulose catabolism at S3 and S4, where the enzymes associated with changes in oligosaccharide anabolism included glycosylase, β-fructosidase and glucan endonuclease. This report will provide a theoretical basis for further elucidating the mechanisms of oligosaccharide anabolism during the growth and development of LBL. |
| first_indexed | 2024-03-13T10:18:56Z |
| format | Article |
| id | doaj.art-8b66f14cf2e649b48c53d5a0629ab6ec |
| institution | Directory Open Access Journal |
| issn | 2666-1543 |
| language | English |
| last_indexed | 2024-03-13T10:18:56Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj.art-8b66f14cf2e649b48c53d5a0629ab6ec2023-05-21T04:35:52ZengElsevierJournal of Agriculture and Food Research2666-15432023-06-0112100612Identification of proteins associated with changes in oligosaccharide metabolism in Lycium barbarum L. fruit by using iTRAQ technologyPengyan Gao0Peipei Li1Zefu Li2Jun Liu3Anran Zheng4Ziyin Hu5Runyu Wang6Dunhua Liu7School of Food Science and Engineering, Ningxia University, Yinchuan, Ningxia, 7500021, China; National Key Laboratory for Market Supervision of Quality and Safety of Goji Berry & Wine, 750021, Yinchuan, ChinaSchool of Food Science and Engineering, Ningxia University, Yinchuan, Ningxia, 7500021, ChinaCOFCO Great Wall Wine Co, Beijing, 100010, ChinaSchool of Agricultural College, Ningxia University, Yinchuan, Ningxia, 7500021, ChinaSchool of Agricultural College, Ningxia University, Yinchuan, Ningxia, 7500021, ChinaSchool of Food Science and Engineering, Ningxia University, Yinchuan, Ningxia, 7500021, ChinaShenzhen Wucheng Equity Investment Co, Shanghai, 200000, ChinaSchool of Food Science and Engineering, Ningxia University, Yinchuan, Ningxia, 7500021, China; National Key Laboratory for Market Supervision of Quality and Safety of Goji Berry & Wine, 750021, Yinchuan, China; School of Agricultural College, Ningxia University, Yinchuan, Ningxia, 7500021, China; Corresponding author. No. 489, Mount Helan West Road, Xixia District, Yinchuan City, 750000, 17 Ningxia Hui Autonomous Region, China.Oligosaccharides are important functional active components of Lycium barbarum L. (LBL, “Goji”) fruits, but their metabolic mechanisms are not yet clear. In this study, we firstly investigated the changes in the content of oligosaccharides in LBL at different stages (S1–S5), and found that it mainly accumulated at S3 and S4. Secondly, the structural characteristics of LBL oligosaccharide (LBO) were studied by GC-MS and FT-IR. Thirdly, using iTRAQ proteomics analysis, we identified 135 differentially expressed proteins associated with oligosaccharide metabolism, which is mainly accomplished through the collaboration of a range of key enzymes. At S1 and S2, oligosaccharide synthesis was achieved mainly through photosynthesis, and changes in anabolism were mainly associated with the expression of sucrose synthase, sucrose phosphate synthase and hexokinase. Oligosaccharide accumulation was achieved through starch and polysaccharide metabolism and cellulose catabolism at S3 and S4, where the enzymes associated with changes in oligosaccharide anabolism included glycosylase, β-fructosidase and glucan endonuclease. This report will provide a theoretical basis for further elucidating the mechanisms of oligosaccharide anabolism during the growth and development of LBL.http://www.sciencedirect.com/science/article/pii/S2666154323001199Lycium barbarum L.OligosaccharidesMetabolismProteomics |
| spellingShingle | Pengyan Gao Peipei Li Zefu Li Jun Liu Anran Zheng Ziyin Hu Runyu Wang Dunhua Liu Identification of proteins associated with changes in oligosaccharide metabolism in Lycium barbarum L. fruit by using iTRAQ technology Journal of Agriculture and Food Research Lycium barbarum L. Oligosaccharides Metabolism Proteomics |
| title | Identification of proteins associated with changes in oligosaccharide metabolism in Lycium barbarum L. fruit by using iTRAQ technology |
| title_full | Identification of proteins associated with changes in oligosaccharide metabolism in Lycium barbarum L. fruit by using iTRAQ technology |
| title_fullStr | Identification of proteins associated with changes in oligosaccharide metabolism in Lycium barbarum L. fruit by using iTRAQ technology |
| title_full_unstemmed | Identification of proteins associated with changes in oligosaccharide metabolism in Lycium barbarum L. fruit by using iTRAQ technology |
| title_short | Identification of proteins associated with changes in oligosaccharide metabolism in Lycium barbarum L. fruit by using iTRAQ technology |
| title_sort | identification of proteins associated with changes in oligosaccharide metabolism in lycium barbarum l fruit by using itraq technology |
| topic | Lycium barbarum L. Oligosaccharides Metabolism Proteomics |
| url | http://www.sciencedirect.com/science/article/pii/S2666154323001199 |
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