Increased Incidence and Plasma-Biofilm Formation Ability of SCCmec Type IV Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Patients With Bacteremia
In Japan, Staphylococcal cassette chromosome mec (SCCmec) type IV methicillin-resistant Staphylococcus aureus (MRSA) is an increasingly prominent cause of bacteremia, but the virulence of most of these strains is unclear. We aimed to investigate the relationship between the molecular characteristics...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-03-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.602833/full |
| _version_ | 1818730613633449984 |
|---|---|
| author | Masakaze Hamada Tetsuo Yamaguchi Ayami Sato Ayami Sato Daisuke Ono Kotaro Aoki Chiaki Kajiwara Soichiro Kimura Tadashi Maeda Masakazu Sasaki Hinako Murakami Yoshikazu Ishii Kazuhiro Tateda Kazuhiro Tateda |
| author_facet | Masakaze Hamada Tetsuo Yamaguchi Ayami Sato Ayami Sato Daisuke Ono Kotaro Aoki Chiaki Kajiwara Soichiro Kimura Tadashi Maeda Masakazu Sasaki Hinako Murakami Yoshikazu Ishii Kazuhiro Tateda Kazuhiro Tateda |
| author_sort | Masakaze Hamada |
| collection | DOAJ |
| description | In Japan, Staphylococcal cassette chromosome mec (SCCmec) type IV methicillin-resistant Staphylococcus aureus (MRSA) is an increasingly prominent cause of bacteremia, but the virulence of most of these strains is unclear. We aimed to investigate the relationship between the molecular characteristics and the ability to form biofilms in the presence of blood plasma (plasma-biofilms) of MRSA strains isolated from bloodstream infections. In this study, the molecular characteristics and biofilms of MRSA strains isolated from blood cultures between 2015 and 2017 were analyzed by PCR-based assays, crystal violet staining, and confocal reflection microscopy methods. Among the 90 MRSA isolates, the detection rate of SCCmec type II clones decreased from 60.7 to 20.6%. The SCCmec type IV clone replaced the SCCmec type II clone as the dominant clone, with a detection rate increasing from 32.1 to 73.5%. The plasma-biofilm formation ability of the SCCmec type IV clone was higher than the SCCmec type II clone and even higher in strains harboring the cna or arcA genes. Plasma-biofilms, mainly composed of proteins, were formed quickly and strongly. Our study demonstrated the increased plasma-biofilm formation ability of SCCmec type IV strains. |
| first_indexed | 2024-12-17T23:04:34Z |
| format | Article |
| id | doaj.art-8b6944e3578041bd8d3772606f2ecf6e |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-17T23:04:34Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-8b6944e3578041bd8d3772606f2ecf6e2022-12-21T21:29:19ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-03-011110.3389/fcimb.2021.602833602833Increased Incidence and Plasma-Biofilm Formation Ability of SCCmec Type IV Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Patients With BacteremiaMasakaze Hamada0Tetsuo Yamaguchi1Ayami Sato2Ayami Sato3Daisuke Ono4Kotaro Aoki5Chiaki Kajiwara6Soichiro Kimura7Tadashi Maeda8Masakazu Sasaki9Hinako Murakami10Yoshikazu Ishii11Kazuhiro Tateda12Kazuhiro Tateda13Department of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, JapanDepartment of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, JapanDepartment of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, JapanDepartment of Surgery, Toho University Sakura Medical Center, Chiba, JapanDepartment of Infectious Diseases and Infection Control, Saitama Medical Center, Saitama Medical University, Saitama, JapanDepartment of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, JapanDepartment of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, JapanDepartment of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, JapanDepartment of General Medicine and Emergency Care, Toho University Omori Medical Center, Tokyo, JapanDepartment of Clinical Laboratories, Toho University Omori Medical Center, Tokyo, JapanDepartment of Clinical Laboratories, Toho University Omori Medical Center, Tokyo, JapanDepartment of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, JapanDepartment of Microbiology and Infectious Diseases, Toho University School of Medicine, Tokyo, JapanDepartment of Clinical Laboratories, Toho University Omori Medical Center, Tokyo, JapanIn Japan, Staphylococcal cassette chromosome mec (SCCmec) type IV methicillin-resistant Staphylococcus aureus (MRSA) is an increasingly prominent cause of bacteremia, but the virulence of most of these strains is unclear. We aimed to investigate the relationship between the molecular characteristics and the ability to form biofilms in the presence of blood plasma (plasma-biofilms) of MRSA strains isolated from bloodstream infections. In this study, the molecular characteristics and biofilms of MRSA strains isolated from blood cultures between 2015 and 2017 were analyzed by PCR-based assays, crystal violet staining, and confocal reflection microscopy methods. Among the 90 MRSA isolates, the detection rate of SCCmec type II clones decreased from 60.7 to 20.6%. The SCCmec type IV clone replaced the SCCmec type II clone as the dominant clone, with a detection rate increasing from 32.1 to 73.5%. The plasma-biofilm formation ability of the SCCmec type IV clone was higher than the SCCmec type II clone and even higher in strains harboring the cna or arcA genes. Plasma-biofilms, mainly composed of proteins, were formed quickly and strongly. Our study demonstrated the increased plasma-biofilm formation ability of SCCmec type IV strains.https://www.frontiersin.org/articles/10.3389/fcimb.2021.602833/fullStaphylococcus aureusStaphylococcal cassette chromosome mec (SCCmec) type IVmethicillin-resistant Staphylococcus aureus (MRSA)biofilmplasmabloodstream infection |
| spellingShingle | Masakaze Hamada Tetsuo Yamaguchi Ayami Sato Ayami Sato Daisuke Ono Kotaro Aoki Chiaki Kajiwara Soichiro Kimura Tadashi Maeda Masakazu Sasaki Hinako Murakami Yoshikazu Ishii Kazuhiro Tateda Kazuhiro Tateda Increased Incidence and Plasma-Biofilm Formation Ability of SCCmec Type IV Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Patients With Bacteremia Frontiers in Cellular and Infection Microbiology Staphylococcus aureus Staphylococcal cassette chromosome mec (SCCmec) type IV methicillin-resistant Staphylococcus aureus (MRSA) biofilm plasma bloodstream infection |
| title | Increased Incidence and Plasma-Biofilm Formation Ability of SCCmec Type IV Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Patients With Bacteremia |
| title_full | Increased Incidence and Plasma-Biofilm Formation Ability of SCCmec Type IV Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Patients With Bacteremia |
| title_fullStr | Increased Incidence and Plasma-Biofilm Formation Ability of SCCmec Type IV Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Patients With Bacteremia |
| title_full_unstemmed | Increased Incidence and Plasma-Biofilm Formation Ability of SCCmec Type IV Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Patients With Bacteremia |
| title_short | Increased Incidence and Plasma-Biofilm Formation Ability of SCCmec Type IV Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Patients With Bacteremia |
| title_sort | increased incidence and plasma biofilm formation ability of sccmec type iv methicillin resistant staphylococcus aureus mrsa isolated from patients with bacteremia |
| topic | Staphylococcus aureus Staphylococcal cassette chromosome mec (SCCmec) type IV methicillin-resistant Staphylococcus aureus (MRSA) biofilm plasma bloodstream infection |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.602833/full |
| work_keys_str_mv | AT masakazehamada increasedincidenceandplasmabiofilmformationabilityofsccmectypeivmethicillinresistantstaphylococcusaureusmrsaisolatedfrompatientswithbacteremia AT tetsuoyamaguchi increasedincidenceandplasmabiofilmformationabilityofsccmectypeivmethicillinresistantstaphylococcusaureusmrsaisolatedfrompatientswithbacteremia AT ayamisato increasedincidenceandplasmabiofilmformationabilityofsccmectypeivmethicillinresistantstaphylococcusaureusmrsaisolatedfrompatientswithbacteremia AT ayamisato increasedincidenceandplasmabiofilmformationabilityofsccmectypeivmethicillinresistantstaphylococcusaureusmrsaisolatedfrompatientswithbacteremia AT daisukeono increasedincidenceandplasmabiofilmformationabilityofsccmectypeivmethicillinresistantstaphylococcusaureusmrsaisolatedfrompatientswithbacteremia AT kotaroaoki increasedincidenceandplasmabiofilmformationabilityofsccmectypeivmethicillinresistantstaphylococcusaureusmrsaisolatedfrompatientswithbacteremia AT chiakikajiwara increasedincidenceandplasmabiofilmformationabilityofsccmectypeivmethicillinresistantstaphylococcusaureusmrsaisolatedfrompatientswithbacteremia AT soichirokimura increasedincidenceandplasmabiofilmformationabilityofsccmectypeivmethicillinresistantstaphylococcusaureusmrsaisolatedfrompatientswithbacteremia AT tadashimaeda increasedincidenceandplasmabiofilmformationabilityofsccmectypeivmethicillinresistantstaphylococcusaureusmrsaisolatedfrompatientswithbacteremia AT masakazusasaki increasedincidenceandplasmabiofilmformationabilityofsccmectypeivmethicillinresistantstaphylococcusaureusmrsaisolatedfrompatientswithbacteremia AT hinakomurakami increasedincidenceandplasmabiofilmformationabilityofsccmectypeivmethicillinresistantstaphylococcusaureusmrsaisolatedfrompatientswithbacteremia AT yoshikazuishii increasedincidenceandplasmabiofilmformationabilityofsccmectypeivmethicillinresistantstaphylococcusaureusmrsaisolatedfrompatientswithbacteremia AT kazuhirotateda increasedincidenceandplasmabiofilmformationabilityofsccmectypeivmethicillinresistantstaphylococcusaureusmrsaisolatedfrompatientswithbacteremia AT kazuhirotateda increasedincidenceandplasmabiofilmformationabilityofsccmectypeivmethicillinresistantstaphylococcusaureusmrsaisolatedfrompatientswithbacteremia |