Development and Application of an SPR Nanobiosensor Based on AuNPs for the Detection of SARS-CoV-2 on Food Surfaces
A new transmission route of SARS-CoV-2 through food was recently considered by the World Health Organization (WHO), and, given the pandemic scenario, the search for fast, sensitive, and low-cost methods is necessary. Biosensors have become a viable alternative for large-scale testing because they ov...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/12/1101 |
| _version_ | 1797461223124500480 |
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| author | Leticia Tessaro Adriano Aquino Pedro Panzenhagen Alan Clavelland Ochioni Yhan S. Mutz Paulo A. Raymundo-Pereira Italo Rennan Sousa Vieira Natasha Kilsy Rocha Belem Carlos Adam Conte-Junior |
| author_facet | Leticia Tessaro Adriano Aquino Pedro Panzenhagen Alan Clavelland Ochioni Yhan S. Mutz Paulo A. Raymundo-Pereira Italo Rennan Sousa Vieira Natasha Kilsy Rocha Belem Carlos Adam Conte-Junior |
| author_sort | Leticia Tessaro |
| collection | DOAJ |
| description | A new transmission route of SARS-CoV-2 through food was recently considered by the World Health Organization (WHO), and, given the pandemic scenario, the search for fast, sensitive, and low-cost methods is necessary. Biosensors have become a viable alternative for large-scale testing because they overcome the limitations of standard techniques. Herein, we investigated the ability of gold spherical nanoparticles (AuNPs) functionalized with oligonucleotides to detect SARS-CoV-2 and demonstrated their potential to be used as plasmonic nanobiosensors. The loop-mediated isothermal amplification (LAMP) technique was used to amplify the viral genetic material from the raw virus-containing solution without any preparation. The detection of virus presence or absence was performed by ultraviolet–visible (UV–Vis) absorption spectroscopy, by monitoring the absorption band of the surface plasmonic resonance (SPR) of the AuNPs. The displacement of the peak by 525 nm from the functionalized AuNPs indicated the absence of the virus (particular region of gold). On the other hand, the region ~300 nm indicated the presence of the virus when RNA bound to the functionalized AuNPs. The nanobiosensor system was designed to detect a region of the N gene in a dynamic concentration range from 0.1 to 50 × 10<sup>3</sup> ng·mL<sup>−1</sup> with a limit of detection (LOD) of 1 ng·mL<sup>−1</sup> (2.7 × 10<sup>3</sup> copy per µL), indicating excellent sensitivity. The nanobiosensor was applied to detect the SARS-CoV-2 virus on the surfaces of vegetables and showed 100% accuracy compared to the standard quantitative reverse transcription polymerase chain reaction (RT-qPCR) technique. Therefore, the nanobiosensor is sensitive, selective, and simple, providing a viable alternative for the rapid detection of SARS-CoV-2 in ready-to-eat vegetables. |
| first_indexed | 2024-03-09T17:16:21Z |
| format | Article |
| id | doaj.art-8b6c080060f84fc9bc35a2e5000a5d43 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T17:16:21Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-8b6c080060f84fc9bc35a2e5000a5d432023-11-24T13:36:32ZengMDPI AGBiosensors2079-63742022-12-011212110110.3390/bios12121101Development and Application of an SPR Nanobiosensor Based on AuNPs for the Detection of SARS-CoV-2 on Food SurfacesLeticia Tessaro0Adriano Aquino1Pedro Panzenhagen2Alan Clavelland Ochioni3Yhan S. Mutz4Paulo A. Raymundo-Pereira5Italo Rennan Sousa Vieira6Natasha Kilsy Rocha Belem7Carlos Adam Conte-Junior8Analytical and Molecular Laboratorial Center (CLAn), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), University City, Rio de Janeiro 21941-909, RJ, BrazilAnalytical and Molecular Laboratorial Center (CLAn), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), University City, Rio de Janeiro 21941-909, RJ, BrazilAnalytical and Molecular Laboratorial Center (CLAn), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), University City, Rio de Janeiro 21941-909, RJ, BrazilAnalytical and Molecular Laboratorial Center (CLAn), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), University City, Rio de Janeiro 21941-909, RJ, BrazilAnalytical and Molecular Laboratorial Center (CLAn), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), University City, Rio de Janeiro 21941-909, RJ, BrazilSão Carlos Institute of Physics (IFSC), University of São Paulo (USP), São Carlos 13566-590, SP, BrazilAnalytical and Molecular Laboratorial Center (CLAn), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), University City, Rio de Janeiro 21941-909, RJ, BrazilLaboratory of Immunogenetics and Molecular Biology of the General Hospital and Maternity Hospital of Cuiabá, Cuiabá 78020-840, MT, BrazilAnalytical and Molecular Laboratorial Center (CLAn), Institute of Chemistry (IQ), Federal University of Rio de Janeiro (UFRJ), University City, Rio de Janeiro 21941-909, RJ, BrazilA new transmission route of SARS-CoV-2 through food was recently considered by the World Health Organization (WHO), and, given the pandemic scenario, the search for fast, sensitive, and low-cost methods is necessary. Biosensors have become a viable alternative for large-scale testing because they overcome the limitations of standard techniques. Herein, we investigated the ability of gold spherical nanoparticles (AuNPs) functionalized with oligonucleotides to detect SARS-CoV-2 and demonstrated their potential to be used as plasmonic nanobiosensors. The loop-mediated isothermal amplification (LAMP) technique was used to amplify the viral genetic material from the raw virus-containing solution without any preparation. The detection of virus presence or absence was performed by ultraviolet–visible (UV–Vis) absorption spectroscopy, by monitoring the absorption band of the surface plasmonic resonance (SPR) of the AuNPs. The displacement of the peak by 525 nm from the functionalized AuNPs indicated the absence of the virus (particular region of gold). On the other hand, the region ~300 nm indicated the presence of the virus when RNA bound to the functionalized AuNPs. The nanobiosensor system was designed to detect a region of the N gene in a dynamic concentration range from 0.1 to 50 × 10<sup>3</sup> ng·mL<sup>−1</sup> with a limit of detection (LOD) of 1 ng·mL<sup>−1</sup> (2.7 × 10<sup>3</sup> copy per µL), indicating excellent sensitivity. The nanobiosensor was applied to detect the SARS-CoV-2 virus on the surfaces of vegetables and showed 100% accuracy compared to the standard quantitative reverse transcription polymerase chain reaction (RT-qPCR) technique. Therefore, the nanobiosensor is sensitive, selective, and simple, providing a viable alternative for the rapid detection of SARS-CoV-2 in ready-to-eat vegetables.https://www.mdpi.com/2079-6374/12/12/1101SPRUV–VisCOVID-19LAMPoptical biosensor |
| spellingShingle | Leticia Tessaro Adriano Aquino Pedro Panzenhagen Alan Clavelland Ochioni Yhan S. Mutz Paulo A. Raymundo-Pereira Italo Rennan Sousa Vieira Natasha Kilsy Rocha Belem Carlos Adam Conte-Junior Development and Application of an SPR Nanobiosensor Based on AuNPs for the Detection of SARS-CoV-2 on Food Surfaces Biosensors SPR UV–Vis COVID-19 LAMP optical biosensor |
| title | Development and Application of an SPR Nanobiosensor Based on AuNPs for the Detection of SARS-CoV-2 on Food Surfaces |
| title_full | Development and Application of an SPR Nanobiosensor Based on AuNPs for the Detection of SARS-CoV-2 on Food Surfaces |
| title_fullStr | Development and Application of an SPR Nanobiosensor Based on AuNPs for the Detection of SARS-CoV-2 on Food Surfaces |
| title_full_unstemmed | Development and Application of an SPR Nanobiosensor Based on AuNPs for the Detection of SARS-CoV-2 on Food Surfaces |
| title_short | Development and Application of an SPR Nanobiosensor Based on AuNPs for the Detection of SARS-CoV-2 on Food Surfaces |
| title_sort | development and application of an spr nanobiosensor based on aunps for the detection of sars cov 2 on food surfaces |
| topic | SPR UV–Vis COVID-19 LAMP optical biosensor |
| url | https://www.mdpi.com/2079-6374/12/12/1101 |
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