Radiation effects of nuclear physics rays on hepatoma cells
The traditional method of cloning formation is used to study the radiation effect of nuclear physics rays on hepatoma cells, and the results obtained are not reliable. Therefore, a new method of studying the radiation effect of nuclear physics rays on hepatoma cells is proposed. PCC method is used t...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2019-04-01
|
| Series: | Open Physics |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/phys-2019-0017 |
| _version_ | 1818689264368484352 |
|---|---|
| author | Zhao Wenhui Cong Lu Guerrero-Sánchez Yolanda |
| author_facet | Zhao Wenhui Cong Lu Guerrero-Sánchez Yolanda |
| author_sort | Zhao Wenhui |
| collection | DOAJ |
| description | The traditional method of cloning formation is used to study the radiation effect of nuclear physics rays on hepatoma cells, and the results obtained are not reliable. Therefore, a new method of studying the radiation effect of nuclear physics rays on hepatoma cells is proposed. PCC method is used to study the radiation effect of γ-rays on hepatoma cells. Radiation effects of X-rays on hepatoma cells were studied by radionuclide formation assay, neutral comet electrophoresis and γH2AX focal detection. The results showed that the survival curves of HepG2 hepatoma cells irradiated by γ-rays were well fitted by linear squares. There was a linear relationship between the survival rate and irradiation dose of HepG2 hepatoma cells irradiated by γ-rays. HepG2 hepatoma cells showed strong tolerance to X-ray irradiation, and the positive rate of γH2AX cells reached 100% in each dose group only 0.5 h after X-ray irradiation. |
| first_indexed | 2024-12-17T12:07:20Z |
| format | Article |
| id | doaj.art-8b7216dcd1f74d70a72eb667c6a4ac70 |
| institution | Directory Open Access Journal |
| issn | 2391-5471 |
| language | English |
| last_indexed | 2024-12-17T12:07:20Z |
| publishDate | 2019-04-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Physics |
| spelling | doaj.art-8b7216dcd1f74d70a72eb667c6a4ac702022-12-21T21:49:34ZengDe GruyterOpen Physics2391-54712019-04-0117116717610.1515/phys-2019-0017phys-2019-0017Radiation effects of nuclear physics rays on hepatoma cellsZhao Wenhui0Cong Lu1Guerrero-Sánchez Yolanda2College of Science, Harbin Engineering University, Harbin, 150001, ChinaCollege of Information and Communication Engineering, Harbin Engineering University, Harbin, 150001, ChinaDepartamento de Demartología, Estomatología y Medicna Física. Área de Medicina Bucal. Universidad de Murcia, Murcia, SpainThe traditional method of cloning formation is used to study the radiation effect of nuclear physics rays on hepatoma cells, and the results obtained are not reliable. Therefore, a new method of studying the radiation effect of nuclear physics rays on hepatoma cells is proposed. PCC method is used to study the radiation effect of γ-rays on hepatoma cells. Radiation effects of X-rays on hepatoma cells were studied by radionuclide formation assay, neutral comet electrophoresis and γH2AX focal detection. The results showed that the survival curves of HepG2 hepatoma cells irradiated by γ-rays were well fitted by linear squares. There was a linear relationship between the survival rate and irradiation dose of HepG2 hepatoma cells irradiated by γ-rays. HepG2 hepatoma cells showed strong tolerance to X-ray irradiation, and the positive rate of γH2AX cells reached 100% in each dose group only 0.5 h after X-ray irradiation.https://doi.org/10.1515/phys-2019-0017γ-rayx-rayhepatoma cellradiation effectcloning formation methodelectrophoresis detectionresearch21.65.+f28.41.te29.30.kv |
| spellingShingle | Zhao Wenhui Cong Lu Guerrero-Sánchez Yolanda Radiation effects of nuclear physics rays on hepatoma cells Open Physics γ-ray x-ray hepatoma cell radiation effect cloning formation method electrophoresis detection research 21.65.+f 28.41.te 29.30.kv |
| title | Radiation effects of nuclear physics rays on hepatoma cells |
| title_full | Radiation effects of nuclear physics rays on hepatoma cells |
| title_fullStr | Radiation effects of nuclear physics rays on hepatoma cells |
| title_full_unstemmed | Radiation effects of nuclear physics rays on hepatoma cells |
| title_short | Radiation effects of nuclear physics rays on hepatoma cells |
| title_sort | radiation effects of nuclear physics rays on hepatoma cells |
| topic | γ-ray x-ray hepatoma cell radiation effect cloning formation method electrophoresis detection research 21.65.+f 28.41.te 29.30.kv |
| url | https://doi.org/10.1515/phys-2019-0017 |
| work_keys_str_mv | AT zhaowenhui radiationeffectsofnuclearphysicsraysonhepatomacells AT conglu radiationeffectsofnuclearphysicsraysonhepatomacells AT guerrerosanchezyolanda radiationeffectsofnuclearphysicsraysonhepatomacells |