A New Validated Potentiometric Method for Sulfite Assay in Beverages Using Cobalt(II) Phthalocyanine as a Sensory Recognition Element
A simple potentiometric sensor is described for accurate, precise, and rapid determination of sulfite additives in beverages. The sensor is based on the use of cobalt phthalocyanine as a recognition material, dispersed in a plasticized poly(vinyl chloride) matrix membrane. <i>o</i>-Nitro...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/13/3076 |
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| author | Saad S. M. Hassan Ayman H. Kamel Abd El-Galil E. Amr Hisham S. M. Abd-Rabboh Mohamed A. Al-Omar Elsayed A. Elsayed |
| author_facet | Saad S. M. Hassan Ayman H. Kamel Abd El-Galil E. Amr Hisham S. M. Abd-Rabboh Mohamed A. Al-Omar Elsayed A. Elsayed |
| author_sort | Saad S. M. Hassan |
| collection | DOAJ |
| description | A simple potentiometric sensor is described for accurate, precise, and rapid determination of sulfite additives in beverages. The sensor is based on the use of cobalt phthalocyanine as a recognition material, dispersed in a plasticized poly(vinyl chloride) matrix membrane. <i>o</i>-Nitrophenyl octyl ether (<i>o</i>-NPOE) as a membrane solvent and tri-dodecylmethyl- ammonium chloride (TDMAC) as ion discriminators are used as membrane additives. Under the optimized conditions, sulfite ion is accurately and precisely measured under batch and flow injection modes of analysis. The sensor exhibits fast and linear response for 1.0 × 10<sup>−2</sup>–1.0 × 10<sup>−6</sup> M (800–0.08 µg/mL) and 1.0 × 10<sup>−1</sup>–5.0 × 10<sup>−5</sup> M (8000–4 µg/mL) sulfite with Nernstian slopes of −27.4 ± 0.3 and −23.7 ± 0.6 mV/concentration decade under static and hydrodynamic modes of operation, respectively. Results in good agreement with the standard iodometric method are obtained.Validation of the assay method is examined in details including precision, accuracy, bias, trueness, repeatability, reproducibility, and uncertainty and good performance characteristics of the method are obtained. The sensor response is stable over the pH range of 5 to 7 without any significant interference from most common anions. The advantages offered by the proposed sensor (i.e., wide range of assay, high accuracy and precision, low detection limit, reasonable selectivity, long term response stability, fast response, and long life span and absence of any sample pretreatment steps) suggest its use in the quality control/quality assurance routine tests in beverages industries, toxicological laboratories and by inspection authorities. |
| first_indexed | 2024-03-10T18:39:40Z |
| format | Article |
| id | doaj.art-8b736ccd05af4ddbb34d2982102bb45c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T18:39:40Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-8b736ccd05af4ddbb34d2982102bb45c2023-11-20T05:59:46ZengMDPI AGMolecules1420-30492020-07-012513307610.3390/molecules25133076A New Validated Potentiometric Method for Sulfite Assay in Beverages Using Cobalt(II) Phthalocyanine as a Sensory Recognition ElementSaad S. M. Hassan0Ayman H. Kamel1Abd El-Galil E. Amr2Hisham S. M. Abd-Rabboh3Mohamed A. Al-Omar4Elsayed A. Elsayed5Department of Chemistry, Faculty of Science, Ain Shams University, Cairo 11566, EgyptDepartment of Chemistry, Faculty of Science, Ain Shams University, Cairo 11566, EgyptPharmaceutical Chemistry Department, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaChemistry Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi ArabiaPharmaceutical Chemistry Department, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaZoology Department, Faculty of Science, King Saud University, Riyadh 11451, Saudi ArabiaA simple potentiometric sensor is described for accurate, precise, and rapid determination of sulfite additives in beverages. The sensor is based on the use of cobalt phthalocyanine as a recognition material, dispersed in a plasticized poly(vinyl chloride) matrix membrane. <i>o</i>-Nitrophenyl octyl ether (<i>o</i>-NPOE) as a membrane solvent and tri-dodecylmethyl- ammonium chloride (TDMAC) as ion discriminators are used as membrane additives. Under the optimized conditions, sulfite ion is accurately and precisely measured under batch and flow injection modes of analysis. The sensor exhibits fast and linear response for 1.0 × 10<sup>−2</sup>–1.0 × 10<sup>−6</sup> M (800–0.08 µg/mL) and 1.0 × 10<sup>−1</sup>–5.0 × 10<sup>−5</sup> M (8000–4 µg/mL) sulfite with Nernstian slopes of −27.4 ± 0.3 and −23.7 ± 0.6 mV/concentration decade under static and hydrodynamic modes of operation, respectively. Results in good agreement with the standard iodometric method are obtained.Validation of the assay method is examined in details including precision, accuracy, bias, trueness, repeatability, reproducibility, and uncertainty and good performance characteristics of the method are obtained. The sensor response is stable over the pH range of 5 to 7 without any significant interference from most common anions. The advantages offered by the proposed sensor (i.e., wide range of assay, high accuracy and precision, low detection limit, reasonable selectivity, long term response stability, fast response, and long life span and absence of any sample pretreatment steps) suggest its use in the quality control/quality assurance routine tests in beverages industries, toxicological laboratories and by inspection authorities.https://www.mdpi.com/1420-3049/25/13/3076sulfite measurementspotentiometrysulfite sensorbeveragesflow injection analysismethod validation |
| spellingShingle | Saad S. M. Hassan Ayman H. Kamel Abd El-Galil E. Amr Hisham S. M. Abd-Rabboh Mohamed A. Al-Omar Elsayed A. Elsayed A New Validated Potentiometric Method for Sulfite Assay in Beverages Using Cobalt(II) Phthalocyanine as a Sensory Recognition Element Molecules sulfite measurements potentiometry sulfite sensor beverages flow injection analysis method validation |
| title | A New Validated Potentiometric Method for Sulfite Assay in Beverages Using Cobalt(II) Phthalocyanine as a Sensory Recognition Element |
| title_full | A New Validated Potentiometric Method for Sulfite Assay in Beverages Using Cobalt(II) Phthalocyanine as a Sensory Recognition Element |
| title_fullStr | A New Validated Potentiometric Method for Sulfite Assay in Beverages Using Cobalt(II) Phthalocyanine as a Sensory Recognition Element |
| title_full_unstemmed | A New Validated Potentiometric Method for Sulfite Assay in Beverages Using Cobalt(II) Phthalocyanine as a Sensory Recognition Element |
| title_short | A New Validated Potentiometric Method for Sulfite Assay in Beverages Using Cobalt(II) Phthalocyanine as a Sensory Recognition Element |
| title_sort | new validated potentiometric method for sulfite assay in beverages using cobalt ii phthalocyanine as a sensory recognition element |
| topic | sulfite measurements potentiometry sulfite sensor beverages flow injection analysis method validation |
| url | https://www.mdpi.com/1420-3049/25/13/3076 |
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